Multigene phylogenetic reconstruction of the Tubulinea (Amoebozoa) corroborates four of the six major lineages, while additionally revealing that shell composition does not predict phylogeny in the Arcellinida.
AuthorsLahr DJ, Grant JR, Katz LA
JournalProtist
PubMed ID23499265
'Tubulinea is a phylogenetically stable higher-level taxon within Amoebozoa, morphologically characterized by monoaxially streaming and cylindrical pseudopods. Contemporary phylogenetic reconstructions have largely relied on SSU rDNA, and to a lesser extent, on actin genes to reveal the relationships among these organisms. Additionally, the test (shell) forming Arcellinida, one of the ... More
Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line.
AuthorsPaulis M, Bensi M, Orioli D, Mondello C, Mazzini G, D'Incalci M, Falcioni C, Radaelli E, Erba E, Raimondi E, De Carli L,
JournalStem Cells
PubMed ID17615268
Two transchromosomic mouse embryonic stem (ES) sublines (ESMClox1.5 and ESMClox2.1) containing a human minichromosome (MC) were established from a sample of hybrid colonies isolated in fusion experiments between a normal diploid mouse ES line and a Chinese hamster ovary line carrying the MC. DNA cytometric and chromosome analyses of ESMClox1.5 ... More
Protein disulfide isomerase-associated 6 is an ATF6-inducible ER stress response protein that protects cardiac myocytes from ischemia/reperfusion-mediated cell death.
AuthorsVekich JA, Belmont PJ, Thuerauf DJ, Glembotski CC
JournalJ Mol Cell Cardiol
PubMed ID22609432
Proper folding of secreted and transmembrane proteins made in the rough endoplasmic reticulum (ER) requires oxygen for disulfide bond formation. Accordingly, ischemia can impair ER protein folding and initiate the ER stress response, which we previously showed is activated in the ischemic heart and in culture cardiac myocytes subjected to ... More