SuperScript™ IV First-Strand Synthesis System with ezDNase™ Enzyme, Each - FAQs

Can I use SuperScript IV First-Strand Synthesis System with ezDNase Enzyme (Cat. No. 18091150, 18091300) for qPCR with a TaqMan assay?

While the SuperScript IV First-Strand Synthesis System with ezDNase Enzyme is compatible with TaqMan assays, for RT-qPCR applications, we recommend using the SuperScript IV VILO Master Mix. The cDNA synthesis reaction setup with this master mix requires fewer pipetting steps and therefore reduces variation in the data.