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引用和文献 (10)
Invitrogen™
BioPrime™ DNA 标记系统
BioPrime™ DNA 标记系统专门设计用于制备生物素化探针。在存在生物素-14-dCTP 的情况下，随机引物（八聚体）经退火处理变为变性 DNA 模板，并通过 Klenow了解更多信息
| 货号 | 数量 |
|---|---|
| 18094011 | 30 次反应 |
货号 18094011
价格（CNY)
8,730.00
Each
数量:
30 次反应
价格（CNY)
8,730.00
Each
BioPrime™ DNA 标记系统专门设计用于制备生物素化探针。在存在生物素-14-dCTP 的情况下，随机引物（八聚体）经退火处理变为变性 DNA 模板，并通过 Klenow 片段扩展，生成灵敏的生物素化 DNA 探针，用于 DNA 和 RNA 的非放射性检测。使用 BioPrime™ DNA 标记系统时，会发生大量净 DNA 合成，从而使探针扩增 10–40 倍。本产品特别适合 DNA 有限的情况。使用本产品制备的探针已用于与 Southern 和 Northern 印迹、菌斑和菌落转移杂交以及与染色体分散原位杂交。BioPrime™ DNA 标记系统：
•需要的模板 DNA 少于切口平移
• 扩增模板以提供更多的生物素化探针
•一次反应中标记50至 500 ng 模板 DNA
•需要的模板 DNA 少于切口平移
• 扩增模板以提供更多的生物素化探针
•一次反应中标记50至 500 ng 模板 DNA
仅供科研使用。不可用于诊断程序。
规格
包括标签或染料是
标记方法直接标记
产品线BioPrime
产品类型DNA 标记系统
数量30 次反应
运输条件经批准可置于湿冰或干冰上运输
检测方法基于生物素
最终产品类型探针（标记 DNA）
标记目标基因组 DNA，DNA（一般）
标签或染料生物素
Unit SizeEach
内容与储存
• 700 µL 2.5X 随机引物溶液
• 175 µL 10X dNTP 混合物
• 100 µg 对照 DNA
• 35 µL Klenow 片段 (40 U/µL)
• 500 µL 终止缓冲液
• 1.25 mL 蒸馏水
储存在 -20°C 非自动除霜冷冻冰箱中。
• 175 µL 10X dNTP 混合物
• 100 µg 对照 DNA
• 35 µL Klenow 片段 (40 U/µL)
• 500 µL 终止缓冲液
• 1.25 mL 蒸馏水
储存在 -20°C 非自动除霜冷冻冰箱中。
常见问题解答 (FAQ)
Can exo-Klenow be purchased separately from your array labeling kits?
引用和文献 (10)
引用和文献
Abstract
A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.
Journal:Genome Res
PubMed ID:14707179
'Microarray-based comparative genomic hybridization (CGH) has become a powerful method for the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been used for mouse CGH studies, the resolving power of these analyses was limited because high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC
DNA replication origins fire stochastically in fission yeast.
Journal:Mol Biol Cell
PubMed ID:16251353
'DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated
A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters.
Journal:Appl Environ Microbiol
PubMed ID:16751532
'Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and
Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.
Journal:Cell
PubMed ID:12086601
'Yeast contains a family of five related histone deacetylases (HDACs) whose functions are known at few genes. Therefore, we used chromatin immunoprecipitation and intergenic microarrays to generate genome-wide HDAC enzyme activity maps. Rpd3 and Hda1 deacetylate mainly distinct promoters and gene classes where they are recruited largely by novel mechanisms.
Characterization of the genome composition of Bartonella koehlerae by microarray comparative genomic hybridization profiling.
Journal:J Bacteriol
PubMed ID:16109957
Bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. We have estimated here the gene content of Bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. The