BioPrime® Plus Array CGH Indirect Genomic Labeling System - FAQs

查看更多产品信息 BioPrime® Plus Array CGH Indirect Genomic Labeling System - FAQs (18096011)

3 个常见问题解答

Can regular Klenow be used for BioPrime Plus Array CGH labeling rather than exo-Klenow?

Regular Klenow may be used but is not recommended because yield, sensitivity, and reproducibility may be compromised compared to exo-Klenow. Exo-Klenow polymerase lacks both 5'-3#146; and 3#146;-5#146; exonuclease activity, producing higher yields of labeled sample than standard Klenow and thereby increasing sensitivity. Exo-Klenow polymerase also incorporates modified nucleotides more effectively than standard Klenow, enabling you to obtain stronger hybridization intensities and greater reproducibility of results. Another advantage with a simplified random priming protocol is that the protocol takes less than three hours.

Can exo-Klenow be purchased separately from your array labeling kits?

Yes, the catalog number for the exo-Klenow is AM2008. The enzyme concentration is 5 U/µL.

Can you use amino allyl dUTP and do indirect fluorescent labeling instead of the Cy3/Cy5 dye-labeled dUTP?

The BioPrime Plus Array CGH Indirect Genomic Labeling System is used for labeling with aminohexylacrylamido(aha)-dUTP. Once aminohexyl-labeled cDNA is synthesized, Alexa Fluor 555 succinimidyl ester and Alexa Fluor 647 succinimidyl ester provided in the kit are coupled, resulting in labeled probe.