Transfection techniques for producing recombinant baculoviruses.
AuthorsTrotter KM, Wood HA
JournalMethods Mol Biol
PubMed ID7620563
A role for calcium in stabilizing transport vesicle coats.
Authors Ahluwalia J P; Topp J D; Weirather K; Zimmerman M; Stamnes M;
JournalJ Biol Chem
PubMed ID11435443
'Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N'',N''-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle ... More
Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Authors Iwabuchi K; Li B; Massa H F; Trask B J; Date T; Fields S;
JournalJ Biol Chem
PubMed ID9748285
'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated ... More
Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.
Authors Schmedtje J F Jr; Ji Y S; Liu W L; DuBois R N; Runge M S;
JournalJ Biol Chem
PubMed ID8995303
'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction ... More
Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.
Authors Yoshimura K; Rosenfeld M A; Nakamura H; Scherer E M; Pavirani A; Lecocq J P; Crystal R G;
JournalNucleic Acids Res
PubMed ID1377820
'As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed of a firefly ... More
The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism.
Authors Dai Yan-Shan; Cserjesi Peter; Markham Bruce E; Molkentin Jeffery D;
JournalJ Biol Chem
PubMed ID11994297
'An intricate array of heterogeneous transcription factors participate in programming tissue-specific gene expression through combinatorial interactions that are unique to a given cell-type. The zinc finger-containing transcription factor GATA4, which is widely expressed in mesodermal and endodermal derived tissues, is thought to regulate cardiac myocyte-specific gene expression through combinatorial interactions ... More
Comparison of the intracellular signaling responses by three chimeric fibroblast growth factor receptors in PC12 cells.
Authors Raffioni S; Thomas D; Foehr E D; Thompson L M; Bradshaw R A;
JournalProc Natl Acad Sci U S A
PubMed ID10377388
'Stably transfected PC12 cell lines expressing similar amounts of chimeric receptors composed of the extracellular domain of the human platelet-derived growth factor (PDGF)beta receptor and the transmembrane and intracellular domains of the fibroblast growth factor receptors (FGFRs) 1, 3, and 4 undergo ligand-induced differentiation. The FGFR1 chimera (PFR1) is the ... More
The intramembrane cleavage site of the amyloid precursor protein depends on the length of its transmembrane domain.
Authors Lichtenthaler Stefan F; Beher Dirk; Grimm Heike S; Wang Rong; Shearman Mark S; Masters Colin L; Beyreuther Konrad;
JournalProc Natl Acad Sci U S A
PubMed ID11805291
'Proteolytic processing of the amyloid precursor protein by beta-secretase generates C99, which subsequently is cleaved by gamma-secretase, yielding the amyloid beta peptide (A beta). This gamma-cleavage occurs within the transmembrane domain (TMD) of C99 and is similar to the intramembrane cleavage of Notch. However, Notch and C99 differ in their ... More
Cationic lipid-mediated delivery and expression of prepro-neuropeptide Y cDNA after intraventricular administration in rat: feasibility and limitations.
AuthorsThorsell A, Blomqvist AG, Heilig M
JournalRegul Pept
PubMed ID8701037
'The utility of in vivo lipofection for delivery and expression of a neuropeptide gene in the adult rat brain was explored. Prepro-neuropeptide Y (NPY) cDNA was cloned into the episomal eucaryotic expression vector pCEP4. This construct was complexed to lipofectamine or lipofectin. Complexed DNA was injected into the lateral ventricles ... More
A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.
AuthorsRose JK, Buonocore L, Whitt MA
JournalBiotechniques
PubMed ID1867862
'One of the most efficient systems for the expression of genes in the cytoplasm of animal cells utilizes a recombinant vaccinia virus encoding the bacteriophage T7 RNA polymerase. Cells infected with this virus are transfected with plasmid DNAs containing the gene to be expressed under T7 promoter control. The major ... More
Role of beta3 integrins in melanoma cell adhesion to activated platelets under flow.
AuthorsFelding-Habermann B, Habermann R, SaldÃvar E, Ruggeri ZM
JournalJ Biol Chem
PubMed ID8621462
'Mechanisms mediating tumor cell attachment to the vessel wall under flow conditions are largely unknown. Therefore we analyzed the ability of human melanoma cells to adhere to an immobilized matrix during blood flow and determined the role of platelets in this process. In a parallel plate flow chamber, M21 melanoma ... More
Differential regulation of vascular endothelial growth factor expression by peroxisome proliferator-activated receptors in bladder cancer cells.
AuthorsFauconnet S, Lascombe I, Chabannes E, Adessi GL, Desvergne B, Wahli W, Bittard H,
JournalJ Biol Chem
PubMed ID11980898
'The growth of any solid tumor depends on angiogenesis. Vascular endothelial growth factor (VEGF) plays a prominent role in vesical tumor angiogenesis regulation. Previous studies have shown that the peroxisome proliferator-activated receptor gamma (PPARgamma) was involved in the angiogenesis process. Here, we report for the first time that in two ... More
Differential regulation of vitronectin in mice and humans in vitro.
Authors Seiffert D; Curriden S A; Jenne D; Binder B R; Loskutoff D J;
JournalJ Biol Chem
PubMed ID8621404
'To define the cis-acting elements involved in the regulation of the murine vitronectin (Vn) gene in inflammation, the 5''-flanking region was isolated, fused to the luciferase reporter gene, and the basal and interleukin 6 (IL-6)-stimulated transcriptional activity was tested in transfection experiments using Hep3B cells. Treatment with IL-6 induced this ... More
Efficient transfection of chicken cells by lipofection, and introduction of transfected blastodermal cells into the embryo.
Authors Brazolot C L; Petitte J N; Etches R J; Verrinder Gibbins A M;
JournalMol Reprod Dev
PubMed ID1751034
'Chicken blastodermal cells (CBCs) and primary chicken fibroblasts (PCFs) have been lipofected with a variety of lacZ constructs encoding Escherichia coli beta-galactosidase (beta-gal). A reporter construct (phspPTlacZpA) containing a mouse heat-shock protein 68 gene (hsp 68) promoter was used to establish conditions for efficient lipofection. The construct, in circular or ... More
Regulation of the human P-selectin promoter by Bcl-3 and specific homodimeric members of the NF-kappa B/Rel family.
Authors Pan J; McEver R P;
JournalJ Biol Chem
PubMed ID7559449
'P-selectin, an adhesion receptor for leukocytes, is constitutively expressed by megakaryocytes and endothelial cells. Synthesis of P-selectin is also increased by some inflammatory mediators. We characterized a previously identified kappa B site (-218GGGGGTGACCCC-207) in the promoter of the human P-selectin gene. The kappa B site was unique in that it ... More
The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.
Authors Münger K; Phelps W C; Bubb V; Howley P M; Schlegel R;
JournalJ Virol
PubMed ID2476573
'The early human papillomavirus type 16 genes that directly participate in the in vitro transformation of primary human keratinocytes have been defined. In the context of the full viral genome, mutations in either the E6 or E7 open reading frame completely abrogated transformation of these cells. Mutations in the E1, ... More
Stimulus-transcription coupling in pheochromocytoma cells. Promoter region-specific activation of chromogranin a biosynthesis.
Authors Tang K; Wu H; Mahata S K; Taupenot L; Rozansky D J; Parmer R J; O'Connor D T;
JournalJ Biol Chem
PubMed ID8910462
'To explore stimulus-transcription coupling in pheochromocytoma cells, we studied the biosynthetic response of chromogranin A, the major soluble protein co-stored and co-released with catecholamines, to chromaffin cells'' physiologic nicotinic cholinergic secretory stimulation. Chromogranin A mRNA showed a time-dependent 3.87-fold response to nicotinic stimulation, and a nuclear run-off experiment indicated that ... More
Expression of the hepatitis C virus genome in rat liver after cationic liposome-mediated in vivo gene transfer.
AuthorsTakehara T, Hayashi N, Miyamoto Y, Yamamoto M, Mita E, Fusamoto H, Kamada T
JournalHepatology
PubMed ID7875673
'The lack of a small animal model of hepatitis C virus (HCV) infection has impeded elucidation of the pathogenesis of HCV. The aim of this study was to develop an HCV-expressing animal model by means of cationic liposome-mediated in vivo gene transfer. To examine the feasibility of this strategy, pActLacZ, ... More
Localization and induced expression of fusion genes in the rat lung.
AuthorsHazinski TA, Ladd PA, DeMatteo CA
JournalAm J Respir Cell Mol Biol
PubMed ID1848084
'Liposome-mediated gene transfer is useful for DNA transfection into cells in culture. We wondered whether this method could be used to introduce new DNA into the intact lung. Fusion genes containing either the Rous sarcoma virus (RSV) promoter or the mouse mammary tumor virus (MMTV) promoter (which contains glucocorticoid response ... More
Lipofection does not require the removal of serum.
Authors Brunette E; Stribling R; Debs R;
JournalNucleic Acids Res
PubMed ID1549487
Researchers showed that Lipofectin, Transfectase and other cationic lipids produce transient CAT activity that is at least as high in the presence of serum (10%) as achieved w/ a totally serum free protocol (w/ MEL and CV-1 cells). Lipid/DNA complexes were formed in the absence of serum prior to ... More
Liposome-mediated DNA uptake by sperm cells.
Authors Bachiller D; Schellander K; Peli J; Rüther U;
JournalMol Reprod Dev
PubMed ID1793596
To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no ... More
Plasmid DNAs directly injected into mouse brain with lipofectin can be incorporated and expressed by brain cells.
AuthorsOno T, Fujino Y, Tsuchiya T, Tsuda M
JournalNeurosci Lett
PubMed ID1710037
In this study, we demonstrated that lipofectin-treated DNAs which were injected into mouse brain could be incorporated and expressed by brain cells. When L7RH-beta gal plasmid DNA harboring E. coli beta-galactosidase gene fused with the nuclear location signal of SV40 T-antigen gene was injected into brains of 1-week-old mice, cells ... More
Suppression of audiogenic epileptic seizures by intracerebral injection of a CCK gene vector.
AuthorsZhang LX, Wu M, Han JS
JournalNeuroreport
PubMed ID1520858
P77PMC rat is a breed of rat with congenital audiogenic seizure (AS). AS attacks could be suppressed by cholecystokinin octapeptide (CCK-8) injected intracerebroventricularly (i.c.v.). In the present study we made i.c.v. injection of plasmid pSV2-beta Gal or pSV2-CCK encapsulated with lipofectin. Expression of pSV2-beta Gal in brain occurred from d ... More
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
AuthorsSharma S, Murai F, Miyanohara A, Friedmann T
JournalProc Natl Acad Sci U S A
PubMed ID9380714
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse ... More
CD36 mediates the In vitro inhibitory effects of thrombospondin-1 on endothelial cells.
Thrombospondin-1 (TSP-1) is a naturally occurring inhibitor of angiogenesis that is able to make normal endothelial cells unresponsive to a wide variety of inducers. Here we use both native TSP-1 and small antiangiogenic peptides derived from it to show that this inhibition is mediated by CD36, a transmembrane glycoprotein found ... More
Association of GAD-65, but not of GAD-67, with the Golgi complex of transfected Chinese hamster ovary cells mediated by the N-terminal region.
AuthorsSolimena M, Aggujaro D, Muntzel C, Dirkx R, Butler M, De Camilli P, Hayday A
JournalProc Natl Acad Sci U S A
PubMed ID8464926
Glutamic acid decarboxylase (GAD) is the enzyme responsible for synthesis of the neurotransmitter gamma-aminobutyric acid in neurons and pancreatic beta cells. It is represented by two isoforms, GAD-65 and GAD-67, which are the products of two different genes and differ substantially only at their N-terminal regions. GAD-65 is a dominant ... More
Induction of vascular endothelial growth factor by tumor necrosis factor alpha in human glioma cells. Possible roles of SP-1.
AuthorsRyuto M, Ono M, Izumi H, Yoshida S, Weich HA, Kohno K, Kuwano M
JournalJ Biol Chem
PubMed ID8910439
The expression of vascular endothelial growth factor (VEGF) has been implicated in brain tumor angiogenesis, and the promoter region for the VEGF gene contains several SP-1 and AP-1 (c-Fos and c-Jun) binding motifs. Among eight human glioma cell lines, cellular mRNA levels of transcription factors SP-1 and AP-1 (c-Fos and ... More
Overexpression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis.
AuthorsXing RH, Rabbani SA
JournalInt J Cancer
PubMed ID8707419
We have examined the role of urokinase receptor (uPAR) in tumor invasion and metastasis by developing a homologous model of uPAR overexpression in a rat breast cancer cell line (Mat B III) using gene transfer technique. Control (pRc-CMV) and experimental plasmid (pRc-uPAR-S) were transfected into Mat B III cells by ... More
Green fluorescent protein tag for studies of drug-induced translocation of nucleolar protein RH-II/Gu.
AuthorsValdez BC, Perlaky L, Cai ZJ, Henning D, Busch H
JournalBiotechniques
PubMed ID9631199
We have constructed a human osteogenic sarcoma cell line, U-2 OS/GFP-Gu, that expresses nucleolar RNA helicase RH-II/Gu tagged with green fluorescent protein (GFP). The presence of a GFP tag does not inhibit RNA helicase, RNA folding and ATPase activities of RH-II/Gu protein. The derived cell line responds to cytotoxic agents ... More
The 3'-untranslated region of membrane exon 2 from the gamma 2a immunoglobulin gene contributes to efficient transcription termination.
AuthorsFlaspohler JA, Boczkowski D, Hall BL, Milcarek C
JournalJ Biol Chem
PubMed ID7744841
Elements of the mouse Immunoglobulin gamma 2a gene, near the membrane-specific poly(A) addition site, were inserted into a heterologous location in either a synthetic mouse gamma 2b gene or a gpt/SV40 chimeric gene and then assayed for their ability to terminate RNA polymerase II transcription in isolated nuclei of transfected ... More
Glycoprotein cytoplasmic domain sequences required for rescue of a vesicular stomatitis virus glycoprotein mutant.
AuthorsWhitt MA, Chong L, Rose JK
JournalJ Virol
PubMed ID2547986
We have used transient expression of the wild-type vesicular stomatitis virus (VSV) glycoprotein (G protein) from cloned cDNA to rescue a temperature-sensitive G protein mutant of VSV in cells at the nonpermissive temperature. Using cDNAs encoding G proteins with deletions in the normal 29-amino-acid cytoplasmic domain, we determined that the ... More
Wild type p53 stimulates expression from the human multidrug resistance promoter in a p53-negative cell line.
AuthorsGoldsmith ME, Gudas JM, Schneider E, Cowan KH
JournalJ Biol Chem
PubMed ID7829527
The effect of human wild type and mutant p53 proteins on the human multidrug resistance (MDR1) promoter was studied in a p53-negative human cell line. Transient expression of MDR1 promoter-chloramphenicol acetyltransferase reporter gene constructs (MDRCAT) cotransfected with p53 expression vectors was analyzed in H358 lung carcinoma cells. Cotransfection with a ... More
Lipofection of cDNAs in the embryonic vertebrate central nervous system.
AuthorsHolt CE, Garlick N, Cornel E
JournalNeuron
PubMed ID1689586
Neurons from the embryonic brain of Xenopus were transfected in vivo with a vector expressing luciferase cDNA using a simple lipofection procedure. Luciferase activity was monitored quantitatively, and the protein was immunolocalized in whole-mount embryonic brains. Luciferase-expressing neurons were often intensely labeled, displaying a Golgi-like filling of their dendrites, axons, ... More
Suppression of postischemic hippocampal nerve growth factor expression by a c-fos antisense oligodeoxynucleotide.
AuthorsCui JK, Hsu CY, Liu PK,
JournalJ Neurosci
PubMed ID9952411
We examined the uptake and distribution of an antisense phosphorothioated oligodeoxynucleotide (s-ODN) to c-fos, rncfosr115, infused into the left cerebral ventricle of male Long-Evans rats and the effect of this s-ODN on subsequent Fos, NGF, neurotrophin-3 (NT-3), and actin expression. To establish the uptake and turnover of s-ODN in the ... More
Molecular cloning and expression of a 58-kDa cis-Golgi and intermediate compartment protein.
AuthorsLahtinen U, Hellman U, Wernstedt C, Saraste J, Pettersson RF
JournalJ Biol Chem
PubMed ID8626736
An abundant 58-kDa (p58) homodimeric and hexameric microsomal membrane protein has been biochemically characterized and localized to tubulo-vesicular elements at the endoplasmic reticulum-Golgi interface and the cis-Golgi cisternae in pancreatic acinar cells (Lahtinen, U., Dahllöf, B., and Saraste, J. (1992) J. Cell Sci. 103, 321-333). Here we report the purification ... More
beta-Thymosins are not simple actin monomer buffering proteins. Insights from overexpression studies.
AuthorsSun HQ, Kwiatkowska K, Yin HL
JournalJ Biol Chem
PubMed ID8621581
beta-Thymosins are the currently favored candidates for maintaining the large actin monomer (G-actin) pool in living cells. To determine if beta-thymosin behaves like a simple G-actin buffering agent in the complex environment of a cell, we overexpressed thymosin beta10 (Tbeta 10) in NIH3T3 cells and determined the effect on the ... More
Therapeutic suppression of translation initiation factor eIF4E expression reduces tumor growth without toxicity.
AuthorsGraff JR, Konicek BW, Vincent TM, Lynch RL, Monteith D, Weir SN, Schwier P, Capen A, Goode RL, Dowless MS, Chen Y, Zhang H, Sissons S, Cox K, McNulty AM, Parsons SH, Wang T, Sams L, Geeganage S, Douglass LE, Neubauer BL, Dean NM, Blanchard K, Shou J, Stancato LF, Carter JH, Marcusson EG,
JournalJ Clin Invest
PubMed ID17786246
Expression of eukaryotic translation initiation factor 4E (eIF4E) is commonly elevated in human and experimental cancers, promoting angiogenesis and tumor growth. Elevated eIF4E levels selectively increase translation of growth factors important in malignancy (e.g., VEGF, cyclin D1) and is thereby an attractive anticancer therapeutic target. Yet to date, no eIF4E-specific ... More
In vivo transposition of Minos, a Drosophila mobile element, in mammalian tissues.
Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. ... More
Growth retardation, polyploidy, and multinucleation induced by Clast3, a novel cell cycle-regulated protein.
AuthorsBahar R, O-Wang J, Kawamura K, Seimiya M, Wang Y, Hatano M, Okada S, Tokuhisa T, Watanabe T, Tagawa M.
JournalJ Biol Chem
PubMed ID12147697
We have identified a novel gene, Clast3, by subtraction of cDNAs derived from activated and naive B lymphocytes. Clast3 expression is elevated in cycling cells and down-regulated in cells undergoing growth arrest, indicating that its expression is controlled in a cell cycle-dependent manner. The deduced amino acid sequence of Clast3 ... More
Distinct roles of the Ikappa B kinase alpha and beta subunits in liberating nuclear factor kappa B (NF-kappa B) from Ikappa B and in phosphorylating the p65 subunit of NF-kappa B.
Authors Sizemore Nywana; Lerner Natalia; Dombrowski Nicole; Sakurai Hiroaki; Stark George R;
JournalJ Biol Chem
PubMed ID11733537
Phosphatidylinositol 3'-kinase (PI3K) and the serine/threonine kinase AKT have critical roles in phosphorylating and transactivating the p65 subunit of nuclear factor kappaB (NF-kappaB) in response to the pro-inflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor (TNF). Mouse embryo fibroblasts (MEFs) lacking either the alpha or beta subunit of IkappaB kinase ... More
Ligand stimulation of a Ret chimeric receptor carrying the activating mutation responsible for the multiple endocrine neoplasia type 2B.
Authors Rizzo C; Califano D; Colucci-D'Amato G L; De Vita G; D'Alessio A; Dathan N A; Fusco A; Monaco C; Santelli G; Vecchio G; Santoro M; de Franciscis V;
JournalJ Biol Chem
PubMed ID8910618
Inherited activating mutations of Ret, a receptor tyrosine kinase, predispose to multiple endocrine neoplasia (MEN) types 2A and 2B and familial medullary thyroid carcinoma. To investigate the effects induced by acute stimulation of Ret, we transfected both PC12 and NIH 3T3 cells with a molecular construct in which the ligand-binding ... More
A novel promoter for vascular endothelial growth factor receptor (flt-1) that confers endothelial-specific gene expression.
Authors Morishita K; Johnson D E; Williams L T;
JournalJ Biol Chem
PubMed ID7499271
The human transmembrane fms-like receptor tyrosine kinase Flt-1 is one of the receptors for vascular endothelial growth factor, a growth factor which induces endothelial proliferation and vascular permeability. Flt-1 is expressed specifically in endothelium and is likely to play a role in tumor angiogenesis and embryonic vascularization. To elucidate the ... More
Liposome-mediated transfection of intact viral particles reveals that plasma membrane penetration determines permissivity of tissue culture cells to rotavirus.
Authors Bass D M; Baylor M R; Chen C; Mackow E M; Bremont M; Greenberg H B;
JournalJ Clin Invest
PubMed ID1334974
Rotaviruses are an important cause of gastroenteritis in human infants. In vivo, rotavirus displays striking cell tropism with viral replication generally restricted to the villus tip enterocytes of the small intestine. We studied a panel of cell lines that vary significantly in their permissivity to rotavirus infection. L cells and ... More
Repression of platelet-derived growth factor A-chain gene transcription by an upstream silencer element. Participation by sequence-specific single-stranded DNA-binding proteins.
Authors Liu B; Maul R S; Kaetzel D M Jr;
JournalJ Biol Chem
PubMed ID8824279
Platelet-derived growth factor A-chain is a potent mitogen expressed in a restricted number of normal and transformed cells. Transient transfection and deletion analysis in BSC-1 (African green monkey, renal epithelial) cells revealed that the -1680 to -1374 region of the A-chain gene repressed homologous and heterologous promoter activities by 60-80%. ... More
Stable production of a human growth hormone antagonist from CHO cells adapted to serum-free suspension culture.
Authors Haldankar R; Kopchick J J; Ridgway D;
JournalBiotechnol Prog
PubMed ID10356250
Human growth hormone (hGH) is a polypeptide with 191 amino acids and a molecular mass of 22 kDa. An hGH analogue was created with a single amino acid substitution (glycine[G] 120 to arginine[R]) in the third alpha-helix of the hGH molecule. This hGH analogue, named hGHG120R, was found to be ... More
A novel abetalipoproteinemia genotype. Identification of a missense mutation in the 97-kDa subunit of the microsomal triglyceride transfer protein that prevents complex formation with protein disulfide isomerase.
Authors Rehberg E F; Samson-Bouma M E; Kienzle B; Blinderman L; Jamil H; Wetterau J R; Aggerbeck L P; Gordon D A;
JournalJ Biol Chem
PubMed ID8939939
The microsomal triglyceride transfer protein (MTP) is a heterodimer composed of the ubiquitous multifunctional protein, protein disulfide isomerase, and a unique 97-kDa subunit. Mutations that lead to the absence of a functional 97-kDa subunit cause abetalipoproteinemia, an autosomal recessive disease characterized by a defect in the assembly and secretion of ... More
Antisense oligonucleotides inhibit intercellular adhesion molecule 1 expression by two distinct mechanisms.
Authors Chiang M Y; Chan H; Zounes M A; Freier S M; Lima W F; Bennett C F;
JournalJ Biol Chem
PubMed ID1680858
Intercellular adhesion molecule 1 (ICAM-1) is a glycoprotein expressed on the surface of both hemopoietic and nonhemopoietic cells that mediates, in part, the emigration of leukocytes out of the vasculature. Expression of ICAM-1 on the surface of human umbilical vein endothelial cells and a human lung carcinoma cell line (A549) ... More
The transcriptional regulation of human aldehyde dehydrogenase I gene. The structural and functional analysis of the promoter.
Authors Yanagawa Y; Chen J C; Hsu L C; Yoshida A;
JournalJ Biol Chem
PubMed ID7615557
Human cytosolic aldehyde dehydrogenase 1 (ALDH1) plays a role in the biosynthesis of retinoic acid that is a modulator for gene expression and cell differentiation. Northern blot analysis showed that liver tissue, pancreas tissue, hepatoma cells, and genital skin fibroblast cells expressed high levels of ALDH1. Sequence analysis showed that ... More
Transcriptional regulation of human prostaglandin-endoperoxide synthase-2 gene by lipopolysaccharide and phorbol ester in vascular endothelial cells. Involvement of both nuclear factor for interleukin-6 expression site and cAMP response element.
There exist two distinct isozymes of prostaglandin-endoperoxide synthase (PES). PES-2 mRNA is synergistically induced by lipopolysaccharide (LPS) and 12-O-tetradecanoylphorbol-13-acetate (TPA) in bovine arterial endothelial cells. On the other hand, PES-1 mRNA is constitutively expressed under these conditions. Therefore, the promoter activities of the human genes for PES-1 and -2 in ... More
Transfection of the human heme oxygenase gene into rabbit coronary microvessel endothelial cells: protective effect against heme and hemoglobin toxicity.
Authors Abraham N G; Lavrovsky Y; Schwartzman M L; Stoltz R A; Levere R D; Gerritsen M E; Shibahara S; Kappas A;
JournalProc Natl Acad Sci U S A
PubMed ID7624322
Heme oxygenase (HO) is a stress protein and has been suggested to participate in defense mechanisms against agents that may induce oxidative injury such as metals, endotoxin, heme/hemoglobin, and various cytokines. Overexpression of HO in cells might therefore protect against oxidative stress produced by certain of these agents, specifically heme ... More
Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides.
Authors Bennett C F; Chiang M Y; Chan H; Shoemaker J E; Mirabelli C K;
JournalMol Pharmacol
PubMed ID1352033
We have investigated the use of a cationic lipid preparation to enhance antisense oligonucleotide activity in human umbilical vein endothelial cells. A liposomal preparation containing the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) was found to increase by at least 1000-fold the potency of an antisense oligonucleotide (ISIS 1570) that hybridizes to ... More
Biological models and genes of tumor reversion: Cellular reprogramming through tpt1/TCTP and SIAH-1.
Tumor reversion is the process by which some cancer cells lose their malignant phenotype. This study was aimed at defining some of the molecular and phenotypic properties of this process. Biological models of tumor reversion were isolated from human leukemia and breast cancer cell lines by using the H-1 parvovirus ... More
Secretion of active human lecithin-cholesterol acyltransferase by insect cells infected with a recombinant baculovirus.
AuthorsChawla D, Owen JS
JournalBiochemical Journal
PubMed ID7619064
Difficulties in purifying the plasma enzyme lecithin-cholesterol acyltransferase (LCAT) have hampered detailed studies of its (patho)physiological role in lipoprotein metabolism and of structure-function relationships. Potentially, baculovirus-driven expression systems offer a powerful means to produce significant amounts of LCAT. Accordingly, full-length LCAT cDNA was cloned into pVL 1392, a high-level expression ... More
The neurotrophic activity of fibroblast growth factor 1 (FGF1) depends on endogenous FGF1 expression and is independent of the mitogen-activated protein kinase cascade pathway.
Authors Renaud F; Desset S; Oliver L; Gimenez-Gallego G; Van Obberghen E; Courtois Y; Laurent M;
JournalJ Biol Chem
PubMed ID8576258
The expression of fibroblast growth factor (FGF) 1, a potent neurotrophic factor, increases during differentiation and remains high in adult neuronal tissues. To examine the importance of this expression on the neuronal phenotype, we have used PC12 cells, a model to study FGF-induced neuronal differentiation. After demonstrating that FGF1 and ... More