ElectroMAX™ DH12S Cells

ElectroMAX™ DH12S Cells - FAQs

查看更多产品信息 ElectroMAX™ DH12S Cells - FAQs (18312017)

2 个常见问题解答

Can a single-stranded DNA be generated using M13 helper phage?

Yes. Use a strain that expresses endonuclease A (e.g., DH12S, DH11S). The orientation of the single-stranded DNA will be the opposite of that generated by Gene II and Exo III.

Can encapsulated phagemid DNA or M13 phage be used to infect bacteria?

Single-stranded DNA viral particles like M13 require the presence of an F pilus in order to infect E. coli. This criterion is met by TOP10F', DH5? F'IQ, INV?F', Stbl4, OmniMAX2-T1 and DH12S cells. These cells are not traD mutants, which effectively allows the cells to retain the F' episome. Transforming single-stranded DNA can cause a 100- to 1,000-fold reduction in efficiency compared to viral particles.