c-Jun is a JNK-independent coactivator of the PU.1 transcription factor.
AuthorsBehre G; Whitmarsh A J; Coghlan M P; Hoang T; Carpenter C L; Zhang D E; Davis R J; Tenen D G;
JournalJ Biol Chem
PubMed ID9988737
The ETS domain transcription factor PU.1 is necessary for the development of monocytes and regulates, in particular, the expression of the monocyte-specific macrophage colony-stimulating factor (M-CSF) receptor, which is critical for monocytic cell survival, proliferation, and differentiation. The bZIP transcription factor c-Jun, which is part of the AP-1 transcription factor ... More
Molecular cloning and characterization of the human prostanoid DP receptor.
AuthorsBoie Y; Sawyer N; Slipetz D M; Metters K M; Abramovitz M;
JournalJ Biol Chem
PubMed ID7642548
A cDNA encoding a functional human prostanoid DP (hDP) receptor has been constructed from a genomic clone and a fragment cloned by 3'-rapid amplification of cDNA ends-polymerase chain reaction. The hDP receptor consists of 359 amino acid residues with a predicted molecular mass of 40,276 and has the putative heptahelical ... More
Reconstitution of a high affinity binding site for type I interferons.
AuthorsRussell-Harde D; Pu H; Betts M; Harkins R N; Perez H D; Croze E;
JournalJ Biol Chem
PubMed ID7592797
The type I interferon (IFN) receptor complex is assumed to be composed of multiple protein subunits. Recently, two proteins have been identified as potential receptor components, both of which share a high degree of structural homology with the immunoglobulin superfamily. One of these proteins, referred to as the human interferon ... More
Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest.
AuthorsBennin David A; Don Aruni S Arachchige; Brake Tiffany; McKenzie Jennifer L; Rosenbaum Heidi; Ortiz Linette; DePaoli-Roach Anna A; Horne Mary C;
JournalJ Biol Chem
PubMed ID11956189
Cyclin G2, together with cyclin G1 and cyclin I, defines a novel cyclin family expressed in terminally differentiated tissues including brain and muscle. Cyclin G2 expression is up-regulated as cells undergo cell cycle arrest or apoptosis in response to inhibitory stimuli independent of p53 (Horne, M., Donaldson, K., Goolsby, G., ... More
The large size (six membrane-spanning repeats in each of four domains) and asymmetric architecture of the voltage-dependent Na+ channel has hindered determination of its structure. With the goal of determining the minimum structure of the Na+ channel permeation pathway, we created two stable cell lines expressing the voltage-dependent rat skeletal ... More
Regulation of Fas expression by STAT3 and c-Jun is mediated by phosphatidylinositol 3-kinase-AKT signaling.
AuthorsIvanov Vladimir N; Krasilnikov Mikhail; Ronai Ze'ev;
JournalJ Biol Chem
PubMed ID11733515
Cooperation between STAT3 and c-Jun results in suppression of Fas Receptor (FasR) transcription, which is often seen in advanced human tumors. To identify requirements for STAT3-Jun cooperation, we elucidated the role of protein kinases that affect both transcription factors. The phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway was found capable of down-regulating ... More
Low-density lipoprotein receptor-related protein 5 (LRP5) is essential for normal cholesterol metabolism and glucose-induced insulin secretion.
AuthorsFujino T, Asaba H, Kang MJ, Ikeda Y, Sone H, Takada S, Kim DH, Ioka RX, Ono M, Tomoyori H, Okubo M, Murase T, Kamataki A, Yamamoto J, Magoori K, Takahashi S, Miyamoto Y, Oishi H, Nose M, Okazaki M, Usui S, Imaizumi K, Yanagisawa M, Sakai J, Yamamoto TT,
JournalProc Natl Acad Sci U S A
PubMed ID12509515
A Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) plays an essential role in bone accrual and eye development. Here, we show that LRP5 is also required for normal cholesterol and glucose metabolism. The production of mice lacking LRP5 revealed that LRP5 deficiency led to increased plasma cholesterol levels in ... More
The C-type lectin receptor Endo180 displays internalization and recycling properties distinct from other members of the mannose receptor family.
AuthorsHoward Matthew J; Isacke Clare M;
JournalJ Biol Chem
PubMed ID12068012
Endo180/urokinase plasminogen activator receptor-associated protein together with the mannose receptor, the phospholipase A(2) receptor, and DEC-205/MR6-gp200 comprise the four members of the mannose receptor family. These receptors have a unique structural composition due to the presence of multiple C-type lectin-like domains within a single polypeptide backbone. In addition, they are ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Molecular cloning and characterization of the human prostanoid DP receptor.
AuthorsBoie Y; Sawyer N; Slipetz D M; Metters K M; Abramovitz M;
JournalJ Biol Chem
PubMed ID7642548
A cDNA encoding a functional human prostanoid DP (hDP) receptor has been constructed from a genomic clone and a fragment cloned by 3'-rapid amplification of cDNA ends-polymerase chain reaction. The hDP receptor consists of 359 amino acid residues with a predicted molecular mass of 40,276 and has the putative heptahelical ... More
Extracellular Export of Sphingosine Kinase-1 Enzyme. SPHINGOSINE 1-PHOSPHATE GENERATION AND THE INDUCTION OF ANGIOGENIC VASCULAR MATURATION.
AuthorsAncellin Nicolas; Colmont Chantal; Su Joseph; Li Qin; Mittereder Nanette; Chae Sung-Suk; Stefansson Steingrimur; Liau Gene; Hla Timothy;
JournalJ Biol Chem
PubMed ID11741921
The enzyme sphingosine kinase (SK) catalyzes the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that acts extracellularly on G protein-coupled receptors of the S1P(1)/EDG-1 subfamily. Although S1P is formed in the cytosol of various cells, S1P release is not understood and is controversial because this lipid mediator is also ... More
c-Jun is a JNK-independent coactivator of the PU.1 transcription factor.
AuthorsBehre G; Whitmarsh A J; Coghlan M P; Hoang T; Carpenter C L; Zhang D E; Davis R J; Tenen D G;
JournalJ Biol Chem
PubMed ID9988737
The ETS domain transcription factor PU.1 is necessary for the development of monocytes and regulates, in particular, the expression of the monocyte-specific macrophage colony-stimulating factor (M-CSF) receptor, which is critical for monocytic cell survival, proliferation, and differentiation. The bZIP transcription factor c-Jun, which is part of the AP-1 transcription factor ... More
Formation and removal of alpha-synuclein aggregates in cells exposed to mitochondrial inhibitors.
Authors Lee He-Jin; Shin Soon Young; Choi Chan; Lee Young Han; Lee Seung-Jae;
JournalJ Biol Chem
PubMed ID11724769
'Mitochondrial dysfunction has been associated with Parkinson''s disease. However, the role of mitochondrial defects in the formation of Lewy bodies, a pathological hallmark of Parkinson''s disease has not been addressed directly. In this report, we investigated the effects of inhibitors of the mitochondrial electron-transport chain on the aggregation of alpha-synuclein, ... More
Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner.
Authors Tan Clara; Mui Alice; Dedhar Shoukat;
JournalJ Biol Chem
PubMed ID11724787
'Nitric oxide (NO) and prostaglandins are produced as a result of the stimulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2, respectively, in response to cytokines or lipopolysaccharide (LPS). We demonstrate that the activity of integrin-linked kinase (ILK) is stimulated by LPS activation in J774 macrophages. Inhibition of ILK activity ... More
MdmX binding to ARF affects Mdm2 protein stability and p53 transactivation.
Authors Jackson M W; Lindstrom M S; Berberich S J;
JournalJ Biol Chem
PubMed ID11297540
'Regulation of p53 involves a complex network of protein interactions. The primary regulator of p53 protein stability is the Mdm2 protein. ARF and MdmX are two proteins that have recently been shown to inhibit Mdm2-mediated degradation of p53 via distinct associations with Mdm2. We demonstrate here that ARF is capable ... More
Suppression of Ras-mediated tumorigenicity and metastasis through inhibition of the Met receptor tyrosine kinase.
Authors Furge K A; Kiewlich D; Le P; Vo M N; Faure M; Howlett A R; Lipson K E; Woude G F; Webb C P;
JournalProc Natl Acad Sci U S A
PubMed ID11535809
'Mutations in the Ras family of GTP binding proteins represent one of the most frequently observed genetic alterations in human cancers. We and others have recently demonstrated that expression of Met, the tyrosine kinase receptor for hepatocyte growth factor/scatter factor (HGF/SF), is significantly up-regulated in Ras-transformed cells. Because HGF/SF-Met signaling ... More
Synaptotagmin V is targeted to dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells.
'Synaptotagmins (Syts) III, V, VI, and X are classified as a subclass of Syt, based on their sequence similarities and biochemical properties (Ibata, K., Fukuda, M., and Mikoshiba, K. (1998) J. Biol. Chem. 273, 12267-12273; Fukuda, M., Kanno, E., and Mikoshiba, K. (1999) J. Biol. Chem. 274, 31421-31427). Although they ... More
G alpha 13 stimulates Na+-H+ exchange through distinct Cdc42-dependent and RhoA-dependent pathways.
Authors Hooley R; Yu C Y; Symons M; Barber D L;
JournalJ Biol Chem
PubMed ID8626403
'Activity of the ubiquitously expressed Na+-H+ exchanger subtype NHE1 is stimulated upon activation of receptor tyrosine kinases and G protein-coupled receptors. The intracellular signaling pathways mediating receptor regulation of the exchanger, however, are poorly understood. Using transient expression of dominant interfering and constitutively active alleles in CCL39 fibroblasts, we determined ... More
Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9.
Authors Boring L; Gosling J; Monteclaro F S; Lusis A J; Tsou C L; Charo I F;
JournalJ Biol Chem
PubMed ID8631787
'We have isolated cDNA clones that encode two closely related, murine C-C chemokine receptors. Both receptors are members of the G-protein-coupled, seven-transmembrane domain family of receptors and are most closely related to the human monocyte chemoattractant protein 1 receptor. Expression of each of the receptors was detected in murine monocyte/macrophage ... More
Functional Re-expression of laminin-5 in laminin-gamma2-deficient human keratinocytes modifies cell morphology, motility, and adhesion.
'Herlitz junctional epidermolysis bullosa (H-JEB) is characterized by a reduced adherence of keratinocytes consequent to deficient expression of the extracellular adhesive ligand laminin-5. To complement the genetic defect causing H-JEB, we transferred an eukaryotic cassette expressing the cDNA for the gamma2 chain of laminin-5 into H-JEB keratinocytes in which the ... More
Src-dependent phosphorylation of the epidermal growth factor receptor on tyrosine 845 is required for zinc-induced Ras activation.
Authors Wu Weidong; Graves Lee M; Gill Gordon N; Parsons Sarah J; Samet James M;
JournalJ Biol Chem
PubMed ID11983694
'Previous studies have shown that exposure of cells to Zn2+ ions induces Ras and MAPK activation through the EGF receptor (EGFR). To further determine the role of EGFR in Zn2+-induced signaling, mouse B82L fibroblasts expressing no detectable EGFR protein (B82L-par), wild type EGFR (B82L-wt), kinase-deficient EGFR (B82L-K721M), or COOH-truncated EGFR ... More
Protective roles of NF-kappa B for chromium(VI)-induced cytotoxicity is revealed by expression of Ikappa B kinase-beta mutant.
Authors Chen Fei; Bower Jacquelyn; Leonard Stephen S; Ding Min; Lu Yongju; Rojanasakul Yon; Kung Hsiang-fu; Vallyathan Val; Castranova Vince; Shi Xianglin;
JournalJ Biol Chem
PubMed ID11726646
'To delineate the molecular mechanisms of NF-kappaB-mediated regulation of chromium(VI)-induced cell death, the signaling pathway leading to the activation of NF-kappaB was interrupted by stable transfection of a kinase-mutated form of IkappaB kinase beta (IKKbeta-KM). Here we demonstrate a novel role for the NF-kappaB transcription factor in inhibiting chromium(VI)-induced cell ... More
Interleukin-1 alpha is released during transfection of keratinocytes.
Authors Komine M; Freedberg I M; Blumenberg M;
JournalJ Invest Dermatol
PubMed ID7930685
'Keratinocytes are known to produce, store, and release IL-1 alpha and therefore we suspected that the DNA-mediated cell transfection procedure may release the stored IL-1 alpha from keratinocytes into the medium. Using enzyme-linked immunosorbent assay, we determined the IL-1 alpha concentration in culture supernatants during keratinocyte transfection. The following transfection ... More
Nonstructural 5A protein of hepatitis C virus modulates tumor necrosis factor alpha-stimulated nuclear factor kappa B activation.
Authors Park Kyu-Jin; Choi Soo-Ho; Lee Soo Young; Hwang Soon B; Lai Michael M C;
JournalJ Biol Chem
PubMed ID11821416
'The hepatitis C virus nonstructural protein 5A (NS5A) is a multifunctional phosphoprotein that leads to pleiotropic responses, in part by regulating cell growth and cellular signaling pathways. Here we show that overexpression of NS5A inhibits tumor necrosis factor (TNF)-alpha-induced nuclear factor kappaB (NF-kappaB) activation in HEK293 cells, as determined by ... More
Residues throughout the cytoplasmic domain affect the internalization efficiency of P-selectin.
Authors Setiadi H; Disdier M; Green S A; Canfield W M; McEver R P;
JournalJ Biol Chem
PubMed ID7592923
'The cytoplasmic domains of many membrane proteins have short sequences, usually including a tyrosine or a di-leucine, that function as sorting signals. P-selectin is an adhesion receptor for leukocytes that is expressed on activated platelets and endothelial cells. Its 35-residue cytoplasmic domain contains signals for sorting into regulated secretory granules, ... More
Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Authors Iwabuchi K; Li B; Massa H F; Trask B J; Date T; Fields S;
JournalJ Biol Chem
PubMed ID9748285
'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated ... More
Osteoprotegerin ligand induces beta-casein gene expression through the transcription factor CCAAT/enhancer-binding protein beta.
Authors Kim Hyun-Ju; Yoon Mi-Jeong; Lee Jinie; Penninger Josef M; Kong Young-Yun;
JournalJ Biol Chem
PubMed ID11726661
'Osteoprotegerin ligand (OPGL, also known as RANKL), a member of the tumor necrosis factor superfamily, is essential for mammary gland development during pregnancy in addition to key roles in the immune system and bone development. Here we show that OPGL induces beta-casein transcription through the CCAAT/enhancer-binding protein beta (C/EBPbeta). In ... More
Two Distinct Regions of the Immunophilin-like Protein XAP2 Regulate Dioxin Receptor Function and Interaction with hsp90.
'The dioxin (aryl hydrocarbon) receptor is a ligand inducible transcription factor, which mediates cellular responses to a variety of xenobiotic compounds such as dioxins. In the absence of ligand the receptor is associated with the molecular chaperone hsp90 and the tetratricopeptide repeat (TPR-) containing immunophilin-like protein XAP2. XAP2 has been ... More
Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3.
Authors Rehm Markus; Dussmann Heiko; Janicke Reiner U; Tavare Jeremy M; Kogel Donat; Prehn Jochen H M;
JournalJ Biol Chem
PubMed ID11964393
'Activation of effector caspases is considered to be the final step in many apoptosis pathways. We transfected HeLa cells with a recombinant caspase substrate composed of cyan and yellow fluorescent protein and a linker peptide containing the caspase cleavage sequence DEVD, and we examined the cleavage kinetics at the single-cell ... More
alpha-Synuclein protects against oxidative stress via inactivation of the c-Jun N-terminal kinase stress-signaling pathway in neuronal cells.
'The expression of alpha-synuclein, a synaptic molecule implicated in the pathogenesis of neurodegenerative disorders such as Parkinson''s disease and Lewy body disease is increased upon injury to the nervous system, indicating that it might play a role in regeneration and plasticity; however, the mechanisms are unclear. Because c-Jun N-terminal kinase ... More
Furin inhibition results in absent or decreased invasiveness and tumorigenicity of human cancer cells.
Authors Bassi D E; Lopez De Cicco R; Mahloogi H; Zucker S; Thomas G; Klein-Szanto A J;
JournalProc Natl Acad Sci U S A
PubMed ID11517338
'Pro-protein convertases such as furin are expressed in many human tumor lines and primary tumors. Furin processes stromelysin-3, membrane type 1 matrix metalloproteinase (MMPs) involved in tumor cell invasiveness, as well as growth factors such as transforming growth factor beta1. Evaluation of furin expression in head and neck squamous cell ... More
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More
Targeting of EBNA1 for rapid intracellular degradation overrides the inhibitory effects of the Gly-Ala repeat domain and restores CD8+ T cell recognition.
Authors Tellam J; Sherritt M; Thomson S; Tellam R; Moss D J; Burrows S R; Wiertz E; Khanna R;
JournalJ Biol Chem
PubMed ID11435434
'Epstein-Barr virus (EBV)-encoded nuclear antigen 1 (EBNA1) includes a unique glycine-alanine repeat domain that inhibits the endogenous presentation of cytotoxic T lymphocyte (CTL) epitopes through the class I pathway by blocking proteasome-dependent degradation of this antigen. This immune evasion mechanism has been implicated in the pathogenesis of EBV-associated diseases. Here, ... More
Ca2+ inhibition of type III adenylyl cyclase in vivo.
Authors Wayman G A; Impey S; Storm D R;
JournalJ Biol Chem
PubMed ID7665559
'Type III adenylyl cyclase is stimulated by beta-adrenergic agonists and glucagon in vitro and in vivo, but not by Ca2+ and calmodulin. However, the enzyme is stimulated by Ca2+ and calmodulin in vitro when it is concomitantly activated by the guanyl nucleotide stimulatory protein Gs (Choi, E. J., Xia, Z., ... More
Essential role of the 58-kDa microspherule protein in the modulation of Daxx-dependent transcriptional repression as revealed by nucleolar sequestration.
Authors Lin Ding-Yen; Shih Hsiu-Ming;
JournalJ Biol Chem
PubMed ID11948183
'Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several lines of evidence have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recent studies have further indicated that Daxx-elicited transcriptional repression can be inhibited by the nuclear body-associated promyelocytic ... More
Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.
Authors Uittenbogaard Annette; Everson William V; Matveev Sergey V; Smart Eric J;
JournalJ Biol Chem
PubMed ID11733519
'We previously demonstrated that in Chinese hamster ovary cells scavenger receptor, class B, type I-dependent selective cholesteryl ester uptake occurs in caveolae. In the present study we hypothesized that cholesteryl ester is transported from caveolae through the cytosol to an internal membrane by a caveolin chaperone complex similar to the ... More
A20 blocks endothelial cell activation through a NF-kappaB-dependent mechanism.
Authors Cooper J T; Stroka D M; Brostjan C; Palmetshofer A; Bach F H; Ferran C;
JournalJ Biol Chem
PubMed ID8663499
'The A20 gene product is a novel zinc finger protein originally described as a tumor necrosis factor alpha (TNF)-inducible early response gene in human umbilical vein endothelial cells (HUVEC). Its described function is to block TNF-induced apoptosis in fibroblasts and B lymphocytes, but more recently it has also been shown ... More
Promoter region of the rat m4 muscarinic acetylcholine receptor gene contains a cell type-specific silencer element.
Authors Mieda M; Haga T; Saffen D W;
JournalJ Biol Chem
PubMed ID8617799
'We describe here the characterization of the rat m4 muscarinic acetylcholine receptor gene and the identification of its regulatory region. Two 5''-noncoding exons are located approximately 5 kilobases upstream from the coding exon, and at least two alternatively spliced variants of m4 mRNA are expressed in the neuronal cell line ... More
p53 Transcriptional activity is mediated through the SRC1-interacting domain of CBP/p300.
Authors Livengood Jill A; Scoggin Kirsten E S; Van Orden Karen; McBryant Steven J; Edayathumangalam Rajeswari S; Laybourn Paul J; Nyborg Jennifer K;
JournalJ Biol Chem
PubMed ID11782467
'The tumor suppressor p53 recruits the cellular coactivator CBP/p300 to mediate the transcriptional activation of target genes. In this study, we identify a novel p53-interacting region in CBP/p300, which we call CR2, located near the carboxyl terminus. The 95-amino acid CR2 region (amino acids 2055--2150) is located adjacent to the ... More
Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.
Authors Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;
JournalJ Biol Chem
PubMed ID12070176
'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More
A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
'Msx2 and Dlx5 are homeodomain proteins that play an important role in osteoblast differentiation and whose expression is induced by bone morphogenetic proteins. Recently we have identified a novel protein, Dlxin-1, that associates with these homeodomain proteins and regulates Dlx5-dependent transcriptional function (Masuda, Y., Sasaki, A., Shibuya, H., Ueno, N., ... More
Hormonal regulation of the human pepsinogen C gene in breast cancer cells. Identification of a cis-acting element mediating its induction by androgens, glucocorticoids, and progesterone.
Authors BalbÃn M; López-OtÃn C;
JournalJ Biol Chem
PubMed ID8663058
'Pepsinogen C is an aspartic proteinase mainly involved in the digestion of proteins in the stomach, which is also synthesized by certain human breast tumors. To examine the possibility that extragastric production of this proteolytic enzyme could be mediated by hormonal factors, we have analyzed pepsinogen C gene expression in ... More
Norepinephrine increases I kappa B alpha expression in astrocytes.
'The neurotransmitter norepinephrine (NE) can inhibit inflammatory gene expression in glial cells; however, the mechanisms involved are not clear. In primary astrocytes, NE dose-dependently increased the expression of inhibitory I kappa B alpha protein accompanied by an increase in steady state levels of I kappa B alpha mRNA. Maximal increases ... More
Protein kinase C and mitogen-activated protein kinase are required for 1,25-dihydroxyvitamin D3-stimulated Egr induction.
Authors Beno D W; Brady L M; Bissonnette M; Davis B H;
JournalJ Biol Chem
PubMed ID7876102
'Recent studies have demonstrated that 1,25-dihydroxyvitamin D3 (D3) can activate Raf kinase and induce Egr expression in cultured rat hepatic Ito cells (Lissoos, T. W., Beno, D. W. A., and Davis, B. H. (1993) J. Biol. Chem. 268, 25132-25138). Since Raf is an upstream activator of mitogen-activated protein kinase (MAPK), ... More
Akt (protein kinase B) negatively regulates SEK1 by means of protein phosphorylation.
Authors Park Hee-Sae; Kim Mi-Sung; Huh Sung-Ho; Park Jihyun; Chung Jongkyeong; Kang Sang Sun; Choi Eui-Ju;
JournalJ Biol Chem
PubMed ID11707464
'The protein serine-threonine kinase Akt mediates cell survival signaling initiated by various growth-promoting factors such as insulin. Here we report that SEK1 is a target of Akt in intact cells. Insulin inhibited the anisomycin-induced stimulation of both endogenous SEK1 and its substrate c-Jun N-terminal kinase (JNK), but not that of ... More
Specific mutations near the amino terminus of double-stranded RNA-dependent protein kinase (PKR) differentially affect its double-stranded RNA binding and dimerization properties.
Authors Patel R C; Stanton P; Sen G C;
JournalJ Biol Chem
PubMed ID8810342
'The amino-terminal region of the double-stranded (ds) RNA-dependent protein kinase, PKR, has been shown to mediate both dsRNA binding and protein dimerization. To critically examine if PKR dimerization is dependent on dsRNA binding, we generated a series of mutants that are incapable of binding dsRNA. Some, but not all, of ... More
Effects of B-Myb on gene transcription: phosphorylation-dependent activity ans acetylation by p300.
Authors Johnson Lance R; Johnson Teresa K; Desler Michelle; Luster Troy A; Nowling Tamara; Lewis Robert E; Rizzino Angie;
JournalJ Biol Chem
PubMed ID11733503
'The transcription factor B-Myb is a cell-cycle regulated phosphoprotein involved in cell cycle progression through the transcriptional regulation of many genes. In this study, we show that the promoter of the fibroblast growth factor-4 (FGF-4) gene is strongly activated by B-Myb in HeLa cells and it can serve as a ... More
Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
Authors Wang Da-Zhi; Li Shijie; Hockemeyer Dirk; Sutherland Lillian; Wang Zhigao; Schratt Gerhard; Richardson James A; Nordheim Alfred; Olson Eric N;
JournalProc Natl Acad Sci U S A
PubMed ID12397177
'Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac ... More
Dopamine beta-monooxygenase signal/anchor sequence alters trafficking of peptidylglycine alpha-hydroxylating monooxygenase.
Authors Oyarce A M; Steveson T C; Jin L; Eipper B A;
JournalJ Biol Chem
PubMed ID11418593
'Dopamine beta-monooxygenase (DBM) and peptidylglycine alpha-hydroxylating monooxygenase (PHM) are essential for the biosynthesis of catecholamines and amidated peptides, respectively. The enzymes share a conserved catalytic core. We studied the role of the DBM signal sequence by appending it to soluble PHM (PHMs) and expressing the DBMsignal/PHMs chimera in AtT-20 and ... More
Transcriptional down-regulation of poly(ADP-ribose) polymerase gene expression by E1A binding to pRb proteins protects murine keratinocytes from radiation-induced apoptosis.
Authors Pacini A; Quattrone A; Denegri M; Fiorillo C; Nediani C; Ramon y Cajal S; Nassi P;
JournalJ Biol Chem
PubMed ID10574992
'Adenovirus E1A confers enhanced cell sensitivity to radiation and drug-induced DNA damage by a mechanism involving the binding to cellular proteins. Mutant analysis in E1A-transfected murine keratinocytes demonstrates that increased sensitivity to DNA damage requires at least E1A binding to the p300/CREB-binding protein (CBP) transcriptional coactivators and to pRb family ... More
Suppression of stellate cell type I collagen gene expression involves AP-2 transmodulation of nuclear factor-1-dependent gene transcription.
Authors Chen A; Beno D W; Davis B H;
JournalJ Biol Chem
PubMed ID8824237
'The regulation of collagen gene expression was studied in culture-activated rat hepatic stellate cells, the fibrogenic effector cell involved in hepatic fibrogenesis. Treatment of cells with a 5-lipoxygenase-specific inhibitor caused a reduction in alphaI(I) collagen mRNA transcript abundance, which suggested that leukotriene production was involved in maintaining the activated cell''s ... More
Glial cell line-derived neurotrophic factor signals through the RET receptor and activates mitogen-activated protein kinase.
Authors Worby C A; Vega Q C; Zhao Y; Chao H H; Seasholtz A F; Dixon J E;
JournalJ Biol Chem
PubMed ID8798576
'Glial cell line-derived neurotrophic factor (GDNF), a member of the transforming growth factor-beta family of growth factors, was first identified by its ability to promote the survival of midbrain dopaminergic neurons in culture. We demonstrate that GDNF treatment of several neuroblastoma cell lines leads to dose-dependent tyrosine phosphorylation of the ... More
Hepatocyte nuclear factor-1 alpha, GATA-4, and caudal related homeodomain protein Cdx2 interact functionally to modulate intestinal gene transcription. Implication for the developmental regulation of the sucrase-isomaltase gene.
Authors Boudreau François; Rings Edmond H H M; van Wering Herbert M; Kim Richard K; Swain Gary P; Krasinski Stephen D; Moffett Jennifer; Grand Richard J; Suh Eun Ran; Traber Peter G;
JournalJ Biol Chem
PubMed ID12060663
'Sucrase-isomaltase (SI), an intestine-specific gene, is induced in the differentiated small intestinal villous epithelium during the suckling-weaning transition in mice. We have previously identified cis-acting elements within a short evolutionarily conserved SI promoter. However, the nature and profile of expression of the interacting proteins have not been fully characterized during ... More
An alternative processing of integrin alpha(v) subunit in tumor cells by membrane type-1 matrix metalloproteinase.
Authors Ratnikov Boris I; Rozanov Dmitri V; Postnova Tanya I; Baciu Peter G; Zhang Heying; DiScipio Richard G; Chestukhina Galina G; Smith Jeffrey W; Deryugina Elena I; Strongin Alex Y;
JournalJ Biol Chem
PubMed ID11741954
'Membrane type-1 matrix metalloproteinase (MT1-MMP) and alpha(v)beta(3) integrin are both essential to cell invasion. Maturation of integrin pro-alpha(v)chain (pro-alpha(v)) involves its cleavage by proprotein convertases (PC) to form the disulfide-bonded 125-kDa heavy and 25-kDa light alpha chains. Our report presents evidence of an alternative pathway of pro-alpha(v) processing involving MT1-MMP. ... More
Different composite regulatory elements direct expression of the human alpha subunit gene to pituitary and placenta.
Authors Heckert L L; Schultz K; Nilson J H;
JournalJ Biol Chem
PubMed ID7592867
'To identify elements of the human alpha subunit gene necessary for cell-specific expression, we generated an array of block mutations spanning approximately 400 base pairs (bp) of promoter proximal region and examined them using transient transfection analysis in pituitary (alpha T3) and placental (BeWo) cell lines. Comparison of promoter activity ... More
Identification of a minimum enhancer sequence for the type II collagen gene reveals several core sequence motifs in common with the link protein gene.
Authors Krebsbach P H; Nakata K; Bernier S M; Hatano O; Miyashita T; Rhodes C S; Yamada Y;
JournalJ Biol Chem
PubMed ID8626777
'The type II collagen gene (Col2a1) is expressed primarily in chondrocytes. Transcription of Col2a1 is mediated by cell-specific regulatory elements located within the promoter and first intron. Here, we map a minimal enhancer and identify elements that determine cartilage-specific Col2a1 expression by analyzing the activity of a series of chimeric ... More
A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression.
Authors Cheng Jie; Moyer Bryan D; Milewski Michal; Loffing Johannes; Ikeda Masahiro; Mickle John E; Cutting Garry R; Li Min; Stanton Bruce A; Guggino William B;
JournalJ Biol Chem
PubMed ID11707463
'We identified a novel cystic fibrosis transmembrane conductance regulator (CFTR)-associating, PDZ domain-containing protein, CAL (CFTR associated ligand) containing two predicted coiled-coiled domains and one PDZ domain. The PDZ domain of CAL binds to the C terminus of CFTR. Although CAL does not have any predicted transmembrane domains, CAL is associated ... More
Fe65, a ligand of the Alzheimer's beta-amyloid precursor protein, blocks cell cycle progression by down-regulating thymidylate synthase expression.
'The functions of the Alzheimer''s beta-amyloid precursor protein (APP) and of its complex with the adaptor protein Fe65 are still unknown. We have demonstrated that Fe65 is also a nuclear protein and APP functions as an extranuclear anchor, thus preventing Fe65 nuclear translocation. According to this finding, it was also ... More
Intracellular third loop domain of angiotensin II type-2 receptor. Role in mediating signal transduction and cellular function.
Authors Hayashida W; Horiuchi M; Dzau V J;
JournalJ Biol Chem
PubMed ID8703004
'The present study tests the hypothesis that the unique intracellular third loop domain of angiotensin II type-2 (AT2) receptor is essential for the subsequent intracellular signaling and plays an important role in mediating receptor function. Synthetic intracellular third loop peptide of the AT2 receptor (AT2-3LP, 22 amino acids) and control ... More
The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism.
Authors Dai Yan-Shan; Cserjesi Peter; Markham Bruce E; Molkentin Jeffery D;
JournalJ Biol Chem
PubMed ID11994297
'An intricate array of heterogeneous transcription factors participate in programming tissue-specific gene expression through combinatorial interactions that are unique to a given cell-type. The zinc finger-containing transcription factor GATA4, which is widely expressed in mesodermal and endodermal derived tissues, is thought to regulate cardiac myocyte-specific gene expression through combinatorial interactions ... More
ATF3 gene. Genomic organization, promoter, and regulation.
Authors Liang G; Wolfgang C D; Chen B P; Chen T H; Hai T;
JournalJ Biol Chem
PubMed ID8576171
'ATF3 gene, which encodes a member of the activating transcription factor/cAMP responsive element binding protein (ATF/CREB) family of transcription factors, is induced by many physiological stresses. As a step toward understanding the induction mechanisms, we isolated the human ATF3 gene and analyzed its genome organization and 5''-flanking region. We found ... More
Protein kinase B/Akt mediates cAMP- and cell swelling-stimulated Na+/taurocholate cotransport and Ntcp translocation.
Authors Webster Cynthia R L; Srinivasulu Usha; Ananthanarayanan Meenakshisundaram; Suchy Frederick J; Anwer M Sawkat;
JournalJ Biol Chem
PubMed ID12034724
'Cyclic AMP and cell swelling stimulate hepatic Na+/TC cotransport and Ntcp translocation via the phosphoinositide 3-kinase signaling pathway. To determine the downstream target of the phosphoinositide 3-kinase action, we examined the role of protein kinase B (PKB)/Akt using SB203580 in hepatocytes as well as by transfection with a dominant negative ... More
Optimizing the cell efficacy of synthetic ribozymes. Site selection and chemical modifications of ribozymes targeting the proto-oncogene c-myb.
Authors Jarvis T C; Wincott F E; Alby L J; McSwiggen J A; Beigelman L; Gustofson J; DiRenzo A; Levy K; Arthur M; Matulic-Adamic J; Karpeisky A; Gonzalez C; Woolf T M; Usman N; Stinchcomb D T;
JournalJ Biol Chem
PubMed ID8910566
'Expression of the proto-oncogene c-myb is necessary for proliferation of vascular smooth muscle cells. We have developed synthetic hammerhead ribozymes that recognize and cleave c-myb RNA, thereby inhibiting cell proliferation. Herein, we describe a method for the selection of hammerhead ribozyme cleavage sites and optimization of chemical modifications that maximize ... More
Androgen Receptor Phosphorylation. REGULATION AND IDENTIFICATION OF THE PHOSPHORYLATION SITES.
Authors Gioeli Daniel; Ficarro Scott B; Kwiek Jesse J; Aaronson David; Hancock Mathew; Catling Andrew D; White Forest M; Christian Robert E; Settlage Robert E; Shabanowitz Jeffrey; Hunt Donald F; Weber Michael J;
JournalJ Biol Chem
PubMed ID12015328
'Activation of signal transduction kinase cascades has been shown to alter androgen receptor (AR) activity. Although it has been suggested that changes in AR phosphorylation might be directly responsible, the basal and regulated phosphorylations of the AR have not been fully determined. We have identified the major sites of AR ... More
Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo.
'A major concern in cancer therapy is resistance of tumors such as glioblastoma to current treatment protocols. Here, we report that transfer of the gene encoding second mitochondria-derived activator of caspase (Smac) or Smac peptides sensitized various tumor cells in vitro and malignant glioma cells in vivo for apoptosis induced ... More
Regulation of human heme oxygenase in endothelial cells by using sense and antisense retroviral constructs.
Authors Quan S; Yang L; Abraham N G; Kappas A;
JournalProc Natl Acad Sci U S A
PubMed ID11593038
'Our objective was to determine whether overexpression and underexpression of human heme oxygenase (HHO)-1 could be controlled on a long-term basis by introduction of the HO-1 gene in sense (S) and antisense (AS) orientation with an appropriate vector into endothelial cells. Retroviral vector (LXSN) containing viral long terminal repeat promoter-driven ... More
Establishment and characterization of cell line LSV5 that retains the altered adhesive properties of human junctional epidermolysis bullosa keratinocytes.
'Herlitz junctional epidermolysis bullosa (H-JEB) is characterized by hampered expression of the adhesion ligand laminin-5. Thus far, analysis of the processes underlying the epithelial-mesenchymal dysadhesion marking this disease has been limited by the reduced growth and adhesive capabilities of the epithelial cells derived from H-JEB patients. To overcome these difficulties, ... More
Repression of Na,K-ATPase beta1-subunit by the transcription factor snail in carcinoma.
AuthorsEspineda CE, Chang JH, Twiss J, Rajasekaran SA, Rajasekaran AK,
JournalMol Biol Cell
PubMed ID14699059
'The Na,K-ATPase consists of two essential alpha- and beta-subunits and regulates the intracellular Na+ and K+ homeostasis. Although the alpha-subunit contains the catalytic activity, it is not active without functional beta-subunit. Here, we report that poorly differentiated carcinoma cell lines derived from colon, breast, kidney, and pancreas show reduced expression ... More
Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell nuclear antigen.
Authors Watanabe H; Pan Z Q; Schreiber-Agus N; DePinho R A; Hurwitz J; Xiong Y;
JournalProc Natl Acad Sci U S A
PubMed ID9465025
'Proper control of the mammalian cell cycle requires the function of cyclin-dependent kinase (CDK) inhibitors. The p21 family currently includes three distinct genes, p21, p27(Kip1), and p57(Kip2), that share a common N-terminal domain for binding to and inhibiting the kinase activity of CDK-cyclin complexes. The p21 protein also binds to ... More
Direct phosphorylation of capsaicin receptor VR1 by protein kinase Cepsilon and identification of two target serine residues.
'The capsaicin receptor, VR1, is a sensory neuron-specific ion channel that serves as a polymodal detector of pain-producing chemical and physical stimuli. It has been reported that ATP, one of the inflammatory mediators, potentiates the VR1 currents evoked by capsaicin or protons and reduces the temperature threshold for activation of ... More
Mechanisms of hepatocyte growth factor stimulation of keratinocyte metalloproteinase production.
Authors Dunsmore S E; Rubin J S; Kovacs S O; Chedid M; Parks W C; Welgus H G;
JournalJ Biol Chem
PubMed ID8798721
'Matrix metalloproteinases participate in normal physiologic processes; however, their overproduction has been associated with connective tissue destruction in a variety of pathological states. Migrating basal keratinocytes transiently express collagenase-1 during normal cutaneous reepithelialization. However, the overexpression of both collagenase-1 and stromelysin-1 has been associated with the pathogenesis of chronic nonhealing ... More
PAX6 gene is activated by the basic helix-loop-helix transcription factor NeuroD/BETA2.
AuthorsMarsich E, Vetere A, Di Piazza M, Tell G, Paoletti S,
JournalBiochem J
PubMed ID12962539
'PAX6 is a transcription factor that plays an important role during pancreas morphogenesis. The aim of this study is to identify the upstream activator(s) of the PAX6 gene possibly involved in the early stage of pancreas differentiation. Recently, individual elements regulating the PAX6 gene activity in the pancreas have been ... More
Interaction between the components of the interferon gamma receptor complex.
AuthorsSerguei V. Kotenko , Lara S. Izotova , Brian P. Pollack , Thomas M. Mariano , Robert J. Donnelly , Geetha Muthukumaran , Jeffry R. Cook , Gianni Garotta, Olli Silvennoinen, James N. Ihle, Sidney Pestka
JournalJ Biol Chem
PubMed ID7673114
'Interferon gamma (IFN-gamma) signals through a multimeric receptor complex consisting of two different chains: the IFN-gamma receptor binding subunit (IFN-gamma R, IFN-gamma R1), and a transmembrane accessory factor (AF-1, IFN-gamma R2) necessary for signal transduction. Using cell lines expressing different cloned components of the IFN-gamma receptor complex, we examined the ... More
The sixth transmembrane domains of the human B1 and B2 bradykinin receptors are structurally compatible and involved in discriminating between subtype-selective agonists.
AuthorsLeeb T, Mathis SA, Leeb-Lundberg LM
JournalJ Biol Chem
PubMed ID8995263
'In order to investigate the molecular basis for the ability of the human B1 and B2 bradykinin (BK) receptor subtypes to discriminate between subtype-selective ligands, we constructed chimeric proteins in which the sixth transmembrane domains (TM-VI) of these receptors were exchanged. The pharmacological profiles of the constructs were analyzed by ... More
Cationic lipid-mediated delivery and expression of prepro-neuropeptide Y cDNA after intraventricular administration in rat: feasibility and limitations.
AuthorsThorsell A, Blomqvist AG, Heilig M
JournalRegul Pept
PubMed ID8701037
'The utility of in vivo lipofection for delivery and expression of a neuropeptide gene in the adult rat brain was explored. Prepro-neuropeptide Y (NPY) cDNA was cloned into the episomal eucaryotic expression vector pCEP4. This construct was complexed to lipofectamine or lipofectin. Complexed DNA was injected into the lateral ventricles ... More
Flavonoid inhibition of sodium-dependent vitamin C transporter 1 (SVCT1) and glucose transporter isoform 2 (GLUT2), intestinal transporters for vitamin C and Glucose.
Authors Song Jian; Kwon Oran; Chen Shenglin; Daruwala Rushad; Eck Peter; Park Jae B; Levine Mark;
JournalJ Biol Chem
PubMed ID11834736
'Vitamin C and flavonoids, polyphenols with uncertain function, are abundant in fruits and vegetables. We postulated that flavonoids have a novel regulatory action of delaying or inhibiting absorption of vitamin C and glucose, which are structurally similar. From six structural classes of flavonoids, at least 12 compounds were chosen for ... More
Livin, a novel inhibitor of apoptosis protein family member.
Authors Kasof G M; Gomes B C;
JournalJ Biol Chem
PubMed ID11024045
'A novel human inhibitor of apoptosis protein (IAP) family member termed Livin was identified, containing a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. The mRNA for livin was not detectable by Northern blot in most normal adult tissues with the exception of the placenta, but ... More
Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans.
'A novel member of the mouse alpha2,8-sialyltransferase (ST8Sia) family, designated ST8Sia VI, was identified by BLAST analysis of expressed sequence tags. The sequence of ST8Sia VI encodes a protein of 398 amino acids and shows 42.0 and 38.3% amino acid sequence identities to mouse alpha2,8-sialyltransferases ST8Sia I (GD3 synthase) and ... More
cGMP-dependent protein kinase inhibits serum-response element-dependent transcription by inhibiting rho activation and functions.
AuthorsGudi T, Chen JC, Casteel DE, Seasholtz TM, Boss GR, Pilz RB,
JournalJ Biol Chem
PubMed ID12119292
'RhoA, in its active GTP-bound form, stimulates transcription through activation of the serum-response factor (SRF). We found that cGMP inhibited serum-induced Rho.GTP loading and transcriptional activation of SRF-dependent reporter genes in smooth muscle and glial cells in a cGMP-dependent protein kinase (G-kinase)-dependent fashion. Serum stimulation of the SRF target gene ... More
Mutation of the cytoplasmic domain of the integrin beta 3 subunit. Differential effects on cell spreading, recruitment to adhesion plaques, endocytosis, and phagocytosis.
Authors Ylänne J; Huuskonen J; O'Toole T E; Ginsberg M H; Virtanen I; Gahmberg C G;
JournalJ Biol Chem
PubMed ID7721884
'The cytoplasmic domain of the beta subunit of the alpha IIb beta 3 integrin is required for cell spreading on fibrinogen. Here we report that deletion of six amino acids from the COOH terminus of the beta 3 (I757TYRGT) totally abolished cell spreading and formation of adhesion plaques, whereas retaining ... More
Optimization of gene transfer using cationic lipids in cell lines and primary human CD4+ and CD34+ hematopoietic cells.
Authors Harrison G S; Wang Y; Tomczak J; Hogan C; Shpall E J; Curiel T J; Felgner P L;
JournalBiotechniques
PubMed ID8588922
'Cationic lipids offer several advantages for gene delivery, both in vitro and in vivo. However, high-efficiency gene transfer has been demonstrated only for limited cell types. Here, we examine the level of expression of a luciferase reporter gene, delivered using cationic lipids, in both cell lines and primary human cells ... More
Secretory leukocyte protease inhibitor mediates proliferation of human endometrial epithelial cells by positive and negative regulation of growth-associated genes.
'Secretory leukocyte protease inhibitor (SLPI) inhibits chymotrypsin, trypsin, elastase, and cathepsin G. This protein also exhibits proliferative effects, although little is known about the molecular mechanisms underlying this activity. We have generated SLPI-ablated epithelial sublines by stably transfecting the Ishikawa human endometrial cell line with an antisense human SLPI RNA ... More
Regulation of Jak kinases by intracellular leptin receptor sequences.
AuthorsKloek C, Haq AK, Dunn SL, Lavery HJ, Banks AS, Myers MG,
JournalJ Biol Chem
PubMed ID12196522
'Leptin signals the status of body energy stores via the leptin receptor (LR), a member of the Type I cytokine receptor family. Type I cytokine receptors mediate intracellular signaling via the activation of associated Jak family tyrosine kinases. Although their COOH-terminal sequences vary, alternatively spliced LR isoforms (LRa-LRd) share common ... More
P38 and activating transcription factor-2 involvement in osteoblast osmotic response to elevated extracellular glucose.
AuthorsZayzafoon M, Botolin S, McCabe LR,
JournalJ Biol Chem
PubMed ID12149242
'Poorly controlled or untreated type I diabetes mellitus is characterized by hyperglycemia and is associated with decreased bone mass and osteoporosis. We have demonstrated that osteoblasts are sensitive to hyperglycemia-associated osmotic stress and respond to elevated extracellular glucose or mannitol by increasing c-jun and collagen I expression. To determine whether ... More
Mutations of two adjacent amino acids generate inactive and constitutively active forms of the human platelet-activating factor receptor.
Authors Parent J L; Le Gouill C; de Brum-Fernandes A J; Rola-Pleszczynski M; Stanková J;
JournalJ Biol Chem
PubMed ID8626474
'We have mutated two residues, Ala230 and Leu231, in the C-terminal portion of the third intracellular loop of the human platelet-activating factor (PAF) receptor into Glu230 and Arg231, respectively. The Leu231 --> Arg231 substitution led to two major modifications: 1) increased constitutive activity of the PAF receptor resulting in agonist-independent ... More
Alternatively spliced products CC3 and TC3 have opposing effects on apoptosis.
AuthorsWhitman S, Wang X, Shalaby R, Shtivelman E.
JournalMol Cell Biol
PubMed ID10611237
'The human gene CC3 is a metastasis suppressor for small cell lung carcinoma (SCLC) in vivo. The ability of CC3 to impair the apoptotic resistance of tumor cells is likely to contribute to metastasis suppression. We describe here an alternatively spliced RNA of CC3, designated TC3, that encodes an unstable ... More
CD14 is a cell-activating receptor for bacterial peptidoglycan.
Authors Gupta D; Kirkland T N; Viriyakosol S; Dziarski R;
JournalJ Biol Chem
PubMed ID8798531
'The hypothesis that CD14 (an endotoxin receptor present on macrophages and neutrophils) acts as a cell-activating receptor for bacterial peptidoglycan was tested using mouse 70Z/3 cells transfected with human CD14. 70Z/3 cells transfected with an empty vector were unresponsive to insoluble and soluble peptidoglycan, as well as to low concentrations ... More
The tumor suppressor protein TSLC1 is involved in cell-cell adhesion.
'TSLC1 is a tumor suppressor gene encoding a member of the immunoglobulin (Ig) superfamily. The significant homology of its extracellular domain with those of other Ig superfamily cell adhesion molecules (IgCAMs) has raised the possibility that TSLC1 participates in cell-cell interactions. In this study, the physiological properties of TSLC1 were ... More
HMGB1 and HMGB2 cell-specifically down-regulate the p53- and p73-dependent sequence-specific transactivation from the human Bax gene promoter.
'The recently cloned gene p73 is a close homologue of p53, which is a crucial tumor suppressor gene for preventing the malignant transformation of cells by inducing cell cycle arrest and apoptosis. Previous reports have shown that architectural DNA-bending/looping chromosomal proteins HMGB1 and HMGB2 (formerly known as HMG1 and HMG2), ... More
Two adaptor proteins differentially modulate the phosphorylation and biophysics of kv1.3 ion channel by SRC kinase.
Authors Cook Karen K; Fadool Debra A;
JournalJ Biol Chem
PubMed ID11812778
'The Shaker family K(+) channel protein, Kv1.3, is tyrosine phosphorylated by v-Src kinase at Tyr(137) and Tyr(449) to modulate current magnitude and kinetic properties. Despite two proline rich sequences and these phosphotyrosines contained in the carboxyl and amino terminals of the channel, v-Src kinase fails to co-immunoprecipitate with Kv1.3 as ... More
The regulated secretion and vectorial targeting of neurotrophins in neuroendocrine and epithelial cells.
Authors Heymach J V Jr; Krüttgen A; Suter U; Shooter E M;
JournalJ Biol Chem
PubMed ID8810312
'The varied roles that neurotrophins play in the development and activity-dependent plasticity of the nervous system presumably require that the sites and quantity of neurotrophin release be precisely regulated. As a step toward understanding how different neurotrophins are sorted and secreted by neurons, we expressed nerve growth factor (NGF), brain-derived ... More
Loricrin expression in cultured human keratinocytes is controlled by a complex interplay between transcription factors of the Sp1, CREB, AP1, and AP2 families.
Authors Jang Shyh-Ing; Steinert Peter M;
JournalJ Biol Chem
PubMed ID12200429
'The major protein component of the cornified cell envelope barrier structure of the epidermis is loricrin, and it is expressed late during terminal differentiation in epidermal keratinocytes. We have previously shown that an AP1 site located in the proximal promoter region (position -55) is essential for human loricrin promoter activity ... More
Ligand binding of the second PDZ domain regulates clustering of PSD-95 with the Kv1.4 potassium channel.
Authors Imamura Fumiaki; Maeda Shoji; Doi Tomoko; Fujiyoshi Yoshinori;
JournalJ Biol Chem
PubMed ID11723117
'The molecular mechanisms underlying the protein assembly at synaptic junctions are thought to be important for neural functions. PSD-95, one of the major postsynaptic density proteins, is composed of three PDZ domains (PDZ1, PDZ2, and PDZ3), an SH3 domain, and a GK (guanylate kinase ) domain. It binds to the ... More
A region of human CD14 required for lipopolysaccharide binding.
Authors Viriyakosol S; Kirkland T N;
JournalJ Biol Chem
PubMed ID7529231
'CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). CD14 binding of LPS is enhanced by serum proteins, especially lipopolysaccharide binding protein. The serum-dependent binding of LPS to CD14 stimulates macrophages to make cytokines, which can cause septic shock in ... More
The proto-oncogene product c-Crk associates with insulin receptor substrate-1 and 4PS. Modulation by insulin growth factor-I (IGF) and enhanced IGF-I signaling.
Authors Beitner-Johnson D; Blakesley V A; Shen-Orr Z; Jimenez M; Stannard B; Wang L M; Pierce J; LeRoith D;
JournalJ Biol Chem
PubMed ID8621590
'The Crk proto-oncogene product is an SH2 and SH3 domain-containing adaptor protein which we have previously shown to become rapidly tyrosine phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) in NIH-3T3 cells. In order to further characterize the role of Crk in the IGF-I signaling pathway, NIH-3T3 ... More
The Binding Site for Channel Blockers That Rescue Misprocessed Human Long QT Syndrome Type 2 ether-a-gogo-related Gene (HERG) Mutations.
Authors Ficker Eckhard; Obejero-Paz Carlos A; Zhao Shuxia; Brown Arthur M;
JournalJ Biol Chem
PubMed ID11741928
'Mutations in the human ether-a-gogo-related gene (HERG) K(+) channel gene cause chromosome 7-linked long QT syndrome type 2 (LQT2), which is characterized by a prolonged QT interval in the electrocardiogram and an increased susceptibility to life-threatening cardiac arrhythmias. LQT2 mutations produce loss-of-function phenotypes and reduce I(Kr) currents either by the ... More
Authors Lin X; Voyno-Yasenetskaya T A; Hooley R; Lin C Y; Orlowski J; Barber D L;
JournalJ Biol Chem
PubMed ID8798430
'Activation of several GTPases stimulates Na+-H+ exchange, resulting in an increased efflux of intracellular H+. These GTPases include alpha subunits of the heterotrimeric G proteins Gq and G13, as well as the low molecular weight GTP-binding proteins Ras, Cdc42, and Rho (Hooley, R., Yu, C.-Y., Simon, M., and Barber, D. ... More