Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA.
AuthorsFlatz L,Bergthaler A,de la Torre JC,Pinschewer DD
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID16537369
The prototypic arenavirus lymphocytic choriomeningitis virus has been a primary workhorse of viral immunologists for almost a century, and it has served as an important model for studying basic principles of arenavirus molecular biology. Its negative-stranded bisegmented RNA genome has, however, posed a major obstacle to attempts at manipulating the ... More
Molecular cloning and characterization of the human prostanoid DP receptor.
AuthorsBoie Y; Sawyer N; Slipetz D M; Metters K M; Abramovitz M;
JournalJ Biol Chem
PubMed ID7642548
A cDNA encoding a functional human prostanoid DP (hDP) receptor has been constructed from a genomic clone and a fragment cloned by 3'-rapid amplification of cDNA ends-polymerase chain reaction. The hDP receptor consists of 359 amino acid residues with a predicted molecular mass of 40,276 and has the putative heptahelical ... More
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
AuthorsWang Qin; Liu Min; Mullah Bashar; Siderovski David P; Neubig Richard R;
JournalJ Biol Chem
PubMed ID12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5, ... More
Low-density lipoprotein receptor-related protein 5 (LRP5) is essential for normal cholesterol metabolism and glucose-induced insulin secretion.
AuthorsFujino T, Asaba H, Kang MJ, Ikeda Y, Sone H, Takada S, Kim DH, Ioka RX, Ono M, Tomoyori H, Okubo M, Murase T, Kamataki A, Yamamoto J, Magoori K, Takahashi S, Miyamoto Y, Oishi H, Nose M, Okazaki M, Usui S, Imaizumi K, Yanagisawa M, Sakai J, Yamamoto TT,
JournalProc Natl Acad Sci U S A
PubMed ID12509515
A Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) plays an essential role in bone accrual and eye development. Here, we show that LRP5 is also required for normal cholesterol and glucose metabolism. The production of mice lacking LRP5 revealed that LRP5 deficiency led to increased plasma cholesterol levels in ... More
A Novel Pathway for Nickel-induced Interleukin-8 Expression.
'Inhalation of particulate nickel subsulfide (Ni(3)S(2)) causes chronic active inflammation and fibrosis of the lungs. However, the mechanisms for these effects are not well understood. Therefore, cell culture experiments with BEAS-2B human airway epithelial cells were conducted to test the hypothesis that exposure to non-cytotoxic levels of Ni(3)S(2) induces expression ... More
SNAP-25 Traffics to the Plasma Membrane by a Syntaxin-independent Mechanism.
Authors Loranger Stephanie S; Linder Maurine E;
JournalJ Biol Chem
PubMed ID12114505
'SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are essential for vesicle docking and fusion. SNAP-25, syntaxin 1A, and synaptobrevin/vesicle-associated membrane protein (VAMP) are SNARE proteins that mediate fusion of synaptic vesicles with the plasma membrane. It has been proposed that interactions of SNAP-25 with syntaxin 1A are required for initial ... More
Intracellular stability of anti-caspase-3 intrabodies determines efficacy in retargeting the antigen.
Authors Rajpal A; Turi T G;
JournalJ Biol Chem
PubMed ID11425853
'Although intracellular antibodies (intrabodies) are being explored as putative therapeutic and research reagents, little is known about the principles that dictate the efficacy of these molecules. In our efforts to address this issue, we generated a panel of five intrabodies, directed against catalytically inactive murine caspase-3, by screening single-chain antibody ... More
Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9.
Authors Boring L; Gosling J; Monteclaro F S; Lusis A J; Tsou C L; Charo I F;
JournalJ Biol Chem
PubMed ID8631787
'We have isolated cDNA clones that encode two closely related, murine C-C chemokine receptors. Both receptors are members of the G-protein-coupled, seven-transmembrane domain family of receptors and are most closely related to the human monocyte chemoattractant protein 1 receptor. Expression of each of the receptors was detected in murine monocyte/macrophage ... More
Ligand receptor interactions in the Wnt signaling pathway in Drosophila.
Authors Wu Chi-hwa; Nusse Roel;
JournalJ Biol Chem
PubMed ID12205098
'Secreted Wnt proteins have numerous signaling functions during development, mediated by Frizzled molecules that act as Wnt receptors on the cell surface. In the genome of Drosophila, seven Wnt genes (including wingless; wg), and five frizzled genes have been identified. Relatively little is known about signaling and binding specificities of ... More
Regulation of pituitary adenylate cyclase-activating polypeptide gene transcription by TTF-1, a homeodomain-containing transcription factor.
Authors Kim Min Sung; Hur Min Kyu; Son Young June; Park Jae-Il; Chun Sang Young; D'Elia Angela Valentina; Damante Giuseppe; Cho Sehyung; Kim Kyungjin; Lee Byung Ju;
JournalJ Biol Chem
PubMed ID12122016
'Pituitary adenylate cyclase-activating polypeptide (PACAP) is an important hypophysiotrophic factor as well as a regulator for immune, reproductive, and neural tissues. We recently found that TTF-1, a homeodomain-containing transcription factor essential for the development of the fetal diencephalon, is postnatally expressed in the hypothalamic area and plays a transcription regulatory ... More
Stepwise contribution of each subunit to the cooperative activation of BK channels by Ca2+.
Authors Niu Xiaowei; Magleby Karl L;
JournalProc Natl Acad Sci U S A
PubMed ID12161564
'BK channels (Slo1) are widely distributed K(+) channels that control Ca(2+)-dependent processes and cellular excitability. Their activation by intracellular Ca(2+) (Ca(i)(2+)) is highly cooperative, with Hill coefficients of typically 2-5. To investigate the cooperativity contributed by each of the four alpha subunits that form the BK channel, we studied single ... More
Identification of the erythropoietin receptor domain required for calcium channel activation.
AuthorsMiller BA, Barber DL, Bell LL, Beattie BK, Zhang MY, Neel BG, Yoakim M, Rothblum LI, Cheung JY
JournalJ Biol Chem
PubMed ID10400674
'Erythropoietin (Epo) activates a voltage-independent Ca2+ channel that is dependent on tyrosine phosphorylation. To identify the domain(s) of the Epo receptor (Epo-R) required for Epo-induced Ca2+ influx, Chinese hamster ovary (CHO) cells were transfected with wild-type or mutant Epo receptors subcloned into pTracer-cytomegalovirus vector. This vector contains an SV40 early ... More
Different composite regulatory elements direct expression of the human alpha subunit gene to pituitary and placenta.
Authors Heckert L L; Schultz K; Nilson J H;
JournalJ Biol Chem
PubMed ID7592867
'To identify elements of the human alpha subunit gene necessary for cell-specific expression, we generated an array of block mutations spanning approximately 400 base pairs (bp) of promoter proximal region and examined them using transient transfection analysis in pituitary (alpha T3) and placental (BeWo) cell lines. Comparison of promoter activity ... More
Tissue-specific expression of the nonneuronal promoter of the aromatic L-amino acid decarboxylase gene is regulated by hepatocyte nuclear factor 1.
Authors Aguanno A; Afar R; Albert V R;
JournalJ Biol Chem
PubMed ID8626808
'The rat aromatic l-amino acid decarboxylase (AADC) gene contains alternative promoters which direct expression of neuronal and nonneuronal mRNAs that differ only in their 5''-untranslated regions (UTRs). We have analyzed the expression of the nonneuronal promoter of the rat AADC gene in the kidney epithelial cell line LLC-PK1 and in ... More
Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells.
Authors Domi Arban; Moss Bernard;
JournalProc Natl Acad Sci U S A
PubMed ID12196634
'The ability to manipulate the vaccinia virus (VAC) genome, as a plasmid in bacteria, would greatly facilitate genetic studies and provide a powerful alternative method of making recombinant viruses. VAC, like other poxviruses, has a linear, double-stranded DNA genome with covalently closed hairpin ends that are resolved from transient head-to-head ... More
ATF3 gene. Genomic organization, promoter, and regulation.
Authors Liang G; Wolfgang C D; Chen B P; Chen T H; Hai T;
JournalJ Biol Chem
PubMed ID8576171
'ATF3 gene, which encodes a member of the activating transcription factor/cAMP responsive element binding protein (ATF/CREB) family of transcription factors, is induced by many physiological stresses. As a step toward understanding the induction mechanisms, we isolated the human ATF3 gene and analyzed its genome organization and 5''-flanking region. We found ... More
Optimizing the cell efficacy of synthetic ribozymes. Site selection and chemical modifications of ribozymes targeting the proto-oncogene c-myb.
Authors Jarvis T C; Wincott F E; Alby L J; McSwiggen J A; Beigelman L; Gustofson J; DiRenzo A; Levy K; Arthur M; Matulic-Adamic J; Karpeisky A; Gonzalez C; Woolf T M; Usman N; Stinchcomb D T;
JournalJ Biol Chem
PubMed ID8910566
'Expression of the proto-oncogene c-myb is necessary for proliferation of vascular smooth muscle cells. We have developed synthetic hammerhead ribozymes that recognize and cleave c-myb RNA, thereby inhibiting cell proliferation. Herein, we describe a method for the selection of hammerhead ribozyme cleavage sites and optimization of chemical modifications that maximize ... More
Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo.
'A major concern in cancer therapy is resistance of tumors such as glioblastoma to current treatment protocols. Here, we report that transfer of the gene encoding second mitochondria-derived activator of caspase (Smac) or Smac peptides sensitized various tumor cells in vitro and malignant glioma cells in vivo for apoptosis induced ... More
CCAAT/Enhancer Binding Protein alpha Binds to the Epstein-Barr Virus (EBV) ZTA Protein through Oligomeric Interactions and Contributes to Cooperative Transcriptional Activation of the ZTA Promoter through Direct Binding to the ZII and ZIIIB Motifs during Induction of the EBV Lytic Cycle.
AuthorsWu FY, Wang SE, Chen H, Wang L, Hayward SD, Hayward GS,
JournalJ Virol
PubMed ID15078966
'The Epstein-Barr virus (EBV)-encoded ZTA protein interacts strongly with and stabilizes the cellular CCAAT/enhancer binding protein alpha (C/EBPalpha), leading to the induction of p21-mediated G(1) cell cycle arrest. Despite the strong interaction between these two basic leucine zipper (bZIP) family proteins, the ZTA and C/EBPalpha subunits do not heterodimerize, as ... More
Inhibition of phosphorylation of BAD and Raf-1 by Akt sensitizes human ovarian cancer cells to paclitaxel.
'We studied the roles of the phosphatidylinositol 3-kinase (PI-3K)-Akt-BAD cascade, ERK-BAD cascade, and Akt-Raf-1 cascade in the paclitaxel-resistant SW626 human ovarian cancer cell line, which lacks functional p53. Treatment of SW626 cells with paclitaxel activates Akt and ERK with different time frames. Interference with the Akt cascade either by treatment ... More
Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34.
'We describe the cloning of a near full-length cDNA of 4258 nucleotides encoding freac-9 (HGMW-approved symbol FKHL17), a novel human forkhead gene. The 5'' untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from ... More
DNA sequence variation in the promoter region of the VEGF gene impacts VEGF gene expression and maximal oxygen consumption.
AuthorsPrior SJ, Hagberg JM, Paton CM, Douglass LW, Brown MD, McLenithan JC, Roth SM,
JournalAm J Physiol Heart Circ Physiol
PubMed ID16339827
'In its role as an endothelial cell proliferation and migration factor, vascular endothelial growth factor (VEGF) can affect peripheral circulation, and therefore impact maximal oxygen consumption (Vo2max). Because of the role of VEGF, and because variation in the VEGF gene has the ability to alter VEGF gene expression and VEGF ... More
Physical interaction of p73 with c-Myc and MM1, a c-Myc-binding protein, and modulation of the p73 function.
'p73 shares high sequence homology with the tumor suppressor p53. Like p53, ectopic overexpression of p73 induces cell cycle arrest and/or apoptosis, and these biological activities are linked to its sequence-specific transactivation function. The COOH-terminal region of p73 is unique and has a function to modulate DNA-binding ability and transactivation ... More
Cationic lipid-mediated delivery and expression of prepro-neuropeptide Y cDNA after intraventricular administration in rat: feasibility and limitations.
AuthorsThorsell A, Blomqvist AG, Heilig M
JournalRegul Pept
PubMed ID8701037
'The utility of in vivo lipofection for delivery and expression of a neuropeptide gene in the adult rat brain was explored. Prepro-neuropeptide Y (NPY) cDNA was cloned into the episomal eucaryotic expression vector pCEP4. This construct was complexed to lipofectamine or lipofectin. Complexed DNA was injected into the lateral ventricles ... More
Intestinal trefoil factor confers colonic epithelial resistance to apoptosis
AuthorsTaupin DR, Kinoshita K, Podolsky DK
JournalProc Natl Acad Sci U S A
PubMed ID10639160
'Intestinal trefoil factor (ITF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution requires the detachment of epithelium from substrate, which would be expected to induce apoptosis. ... More
Cloning and characterization of a novel transcriptional repressor of the nicotinic acetylcholine receptor delta-subunit gene.
Authors Sapru M K; Gao J P; Walke W; Burmeister M; Goldman D;
JournalJ Biol Chem
PubMed ID8636158
'We have identified a negative cis-acting regulatory element in the nicotinic acetylcholine receptor delta-subunit gene''s promoter. This element resides within a previously identified 47-base pair activity-dependent enhancer. Proteins that bind this region of DNA were cloned from a lambdagt11 innervated muscle expression library. Two cDNAs (MY1 and MY1a) were isolated ... More
Mutation of the cytoplasmic domain of the integrin beta 3 subunit. Differential effects on cell spreading, recruitment to adhesion plaques, endocytosis, and phagocytosis.
Authors Ylänne J; Huuskonen J; O'Toole T E; Ginsberg M H; Virtanen I; Gahmberg C G;
JournalJ Biol Chem
PubMed ID7721884
'The cytoplasmic domain of the beta subunit of the alpha IIb beta 3 integrin is required for cell spreading on fibrinogen. Here we report that deletion of six amino acids from the COOH terminus of the beta 3 (I757TYRGT) totally abolished cell spreading and formation of adhesion plaques, whereas retaining ... More
P38 and activating transcription factor-2 involvement in osteoblast osmotic response to elevated extracellular glucose.
AuthorsZayzafoon M, Botolin S, McCabe LR,
JournalJ Biol Chem
PubMed ID12149242
'Poorly controlled or untreated type I diabetes mellitus is characterized by hyperglycemia and is associated with decreased bone mass and osteoporosis. We have demonstrated that osteoblasts are sensitive to hyperglycemia-associated osmotic stress and respond to elevated extracellular glucose or mannitol by increasing c-jun and collagen I expression. To determine whether ... More
p38 kinase regulates nitric oxide-induced apoptosis of articular chondrocytes by accumulating p53 via NFkappa B-dependent transcription and stabilization by serine 15 phosphorylation.
Authors Kim Song-Ja; Hwang Sang-Gu; Shin Deug Y; Kang Shin-Sung; Chun Jang-Soo;
JournalJ Biol Chem
PubMed ID12091386
'Nitric oxide (NO) during primary culture of articular chondrocytes causes apoptosis via p38 mitogen-activated protein kinase in association with elevation of p53 protein level, caspase-3 activation, and differentiation status. In this study, we characterized the molecular mechanism by which p38 kinase induces apoptosis through activation of p53. We report here ... More
Inhibition of Akt kinase by cell-permeable ceramide and its implications for ceramide-induced apoptosis.
Authors Zhou H; Summers S A; Birnbaum M J; Pittman R N;
JournalJ Biol Chem
PubMed ID9632728
'Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. However, mechanisms responsible for ceramide-induced apoptosis remain unclear. We investigated the possibility that ceramide may decrease antiapoptotic signaling in cells by inhibiting Akt kinase activity. Our data show that C2-ceramide induces apoptosis in HMN1 ... More
A region of human CD14 required for lipopolysaccharide binding.
Authors Viriyakosol S; Kirkland T N;
JournalJ Biol Chem
PubMed ID7529231
'CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). CD14 binding of LPS is enhanced by serum proteins, especially lipopolysaccharide binding protein. The serum-dependent binding of LPS to CD14 stimulates macrophages to make cytokines, which can cause septic shock in ... More
Caspase 3 activity is required for skeletal muscle differentiation.
Authors Fernando Pasan; Kelly John F; Balazsi Kim; Slack Ruth S; Megeney Lynn A;
JournalProc Natl Acad Sci U S A
PubMed ID12177420
'The cellular alterations associated with skeletal muscle differentiation share a high degree of similarity with key phenotypic changes usually ascribed to apoptosis. For example, actin fiber disassembly/reorganization is a conserved feature of both apoptosis and differentiating myoblasts and the conserved muscle contractile protein, myosin light chain kinase, is required for ... More
Silencer elements modulate the expression of the gene for the neuron-glia cell adhesion molecule, Ng-CAM.
Authors Kallunki P; Jenkinson S; Edelman G M; Jones F S;
JournalJ Biol Chem
PubMed ID7545667
'The combined factors that regulate the expression of cell adhesion molecules (CAMs) during development of the nervous system are largely unknown. To identify such factors for Ng-CAM, the neuron-glia CAM, constructs containing portions of the 5'' end of the Ng-CAM gene were examined for activity after transfection into N2A neuroblastoma ... More
Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery.
Authors Geyer Matthias; Yu Haifeng; Mandic Robert; Linnemann Thomas; Zheng Yong-Hui; Fackler Oliver T; Peterlin B Matija;
JournalJ Biol Chem
PubMed ID12032142
'Nef is an accessory protein of human and simian immunodeficiency viruses (HIV and SIV) that is required for efficient viral infectivity and pathogenicity. It decreases the expression of CD4 on the surface of infected cells. V1H is the regulatory subunit H of the vacuolar membrane ATPase (V-ATPase). Previously, the interaction ... More
5'-,3'-inverted thymidine-modified antisense oligodeoxynucleotide targeting midkine. Its design and application for cancer therapy.
'Oligodeoxynucleotides modified at both 5''- and 3''-ends with inverted thymidine (5''-,3''-inverted T) were introduced as new reagents for antisense strategies. These modifications were performed to make the oligodeoxynucleotides resistant to nucleases. The effectiveness of these oligodeoxynucleotides was evaluated in terms of inhibition of synthesis of midkine (MK), a heparin-binding growth ... More
Isolation and characterization of the 5'-upstream region of the human N-type calcium channel alpha1B subunit gene. Chromosomal localization and promoter analysis.
Authors Kim D S; Jung H H; Park S H; Chin H;
JournalJ Biol Chem
PubMed ID9030575
'omega-Conotoxin-sensitive N-type Ca2+ channels, unlike dihydropyridine-sensitive L-type channels, are exclusively expressed in nervous tissues. To understand the molecular basis for neuron-specific expression of the N-type channel, we have isolated genomic clones encoding the human alpha1B subunit gene, localized to the long arm of chromosome 9 (9q34) by fluorescence in situ ... More
Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.
'Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell ... More
Altered carbohydrate recognition specificity engineered into surfactant protein D reveals different binding mechanisms for phosphatidylinositol and glucosylceramide.
Authors Ogasawara Y; Voelker D R;
JournalJ Biol Chem
PubMed ID7782337
'Pulmonary surfactant protein D (SP-D) is a member of the collection subgroup of the C-type lectin superfamily that binds glycosylated lipids such as phosphatidylinositol (PI) and glucosylceramide (GlcCer). We have previously reported that the carbohydrate recognition domain of SP-D plays an essential role in lipid binding. However, it is unclear ... More
ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.
Authors Duan H; Chinnaiyan A M; Hudson P L; Wing J P; He W W; Dixit V M;
JournalJ Biol Chem
PubMed ID8576161
'Members of the ICE/ced-3 gene family have been implicated as components of the cell death pathway. Based on similarities with the structural prototype interleukin-1 beta-converting enzyme (ICE), family members are synthesized as proenzymes that are proteolytically processed to form active heterodimeric enzymes. In this report, we describe a novel member ... More
Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14.
AuthorsAmes RS, Sarau HM, Chambers JK, Willette RN, Aiyar NV, Romanic AM, Louden CS, Foley JJ, Sauermelch CF, Coatney RW, Ao Z, Disa J, Holmes SD, Stadel JM, Martin JD, Liu WS, Glover GI, Wilson S, McNulty DE, Ellis CE, Elshourbagy NA, Shabon U, Trill JJ, Hay DW, Douglas SA, et al
JournalNature
PubMed ID10499587
'Urotensin-II (U-II) is a vasoactive ''somatostatin-like'' cyclic peptide which was originally isolated from fish spinal cords, and which has recently been cloned from man. Here we describe the identification of an orphan human G-protein-coupled receptor homologous to rat GPR14 and expressed predominantly in cardiovascular tissue, which functions as a U-II ... More
The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells.
AuthorsZhang W, Lane RD, Mellgren RL
JournalJ Biol Chem
PubMed ID8702541
'Calpains are intracellular Ca2+-dependent proteases that are thought to participate in Ca2+-associated signal transduction pathways. It has been proposed that calpains are activated by an autoproteolytic mechanism. If this is true one would expect a relatively short half-life for calpain protein in cells. To test this hypothesis, WI-38 human diploid ... More
Use of a promoterless Renilla luciferase vector as an internal control plasmid for transient co-transfection assays of Ras-mediated transcription activation.
Authors Behre G; Smith L T; Tenen D G;
JournalBiotechniques
PubMed ID9894587
'not available'
Molecular cloning and expression of a third type of rabbit GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase.
'Recent molecular investigation revealed that two closely related structural genes encode distinct GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferases (alpha1,2-fucosyltransferases). Some human cancer cells or tissues may express an aberrant alpha1, 2-fucosyltransferase other than H- and Secretor-type alpha1, 2-fucosyltransferase. However, definite evidence of the existence of a third type of alpha1,2-fucosyltransferase has not been demonstrated. ... More
p53 suppresses the c-Myb-induced activation of heat shock transcription factor 3.
'Expression of heat shock proteins (HSPs) is controlled by heat shock transcription factors (HSFs). Vertebrates express multiple HSFs whose activities may be regulated by distinct signals. HSF3 is specifically activated in unstressed proliferating cells by direct binding to the c-myb proto-oncogene product (c-Myb), which plays an important role in cellular ... More
Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).
Authors Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;
JournalJ Biol Chem
PubMed ID10956661
'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More
Expression of neuronal traits in pancreatic beta cells. Implication of neuron-restrictive silencing factor/repressor element silencing transcription factor, a neuron-restrictive silencer.
Authors Atouf F; Czernichow P; Scharfmann R;
JournalJ Biol Chem
PubMed ID8999882
'Pancreatic beta cells (insulin-producing cells) and neuronal cells share a large number of similarities. Here, we investigate whether the same mechanisms could control the expression of neuronal genes in both neurons and insulin-producing cells. For that purpose, we tested the role of the transcriptional repressor neuron-restrictive silencing factor/repressor element silencing ... More
Characterization of the human cyclin-dependent kinase 2 gene. Promoter analysis and gene structure.
Authors Shiffman D; Brooks E E; Brooks A R; Chan C S; Milner P G;
JournalJ Biol Chem
PubMed ID8647814
'Cyclin-dependent kinase 2 is a serine/threonine protein kinase essential for progression of the mammalian cell cycle from G1 to S phase. CDK2 mRNA has been shown to be induced by serum in several cultured cell types. Therefore, we set out to identify elements that regulate the transcription of the human ... More
Regulation of gene expression by cGMP-dependent protein kinase. Transactivation of the c-fos promoter.
Authors Gudi T; Huvar I; Meinecke M; Lohmann S M; Boss G R; Pilz R B;
JournalJ Biol Chem
PubMed ID8617718
'The cAMP/cAMP-dependent protein kinase (A-kinase) and Ca2+/calmodulin-dependent protein kinase (Cam-kinase) signal transduction pathways are well known to regulate gene transcription, but this has not been demonstrated directly for the cGMP/cGMP-dependent protein kinase (G-kinase) signal transduction pathway. Here we report that transfection of G-kinase into G-kinase-deficient cells causes activation of the ... More
p73beta Is Regulated by Protein Kinase Cdelta Catalytic Fragment Generated in the Apoptotic Response to DNA Damage.
'Protein kinase C (PKC) delta is cleaved by caspase-3 to a kinase-active catalytic fragment (PKCdeltaCF) in the apoptotic response of cells to DNA damage. Expression of PKCdeltaCF contributes to the induction of apoptosis by mechanisms that are presently unknown. Here we demonstrate that PKCdeltaCF associates with p73beta, a structural and ... More
Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death.
Authors Liu Z G; Hsu H; Goeddel D V; Karin M;
JournalCell
PubMed ID8898208
'Through its type 1 receptor (TNFR1), the cytokine TNF elicits an unusually wide range of biological responses, including inflammation, tumor necrosis, cell proliferation, differentiation, and apoptosis. We investigated how TNFR1 activates different effector functions; the protein kinase JNK, transcription factor NF-kappaB, and apoptosis. We found that the three responses are ... More
Identification of semaphorin E as a non-MDR drug resistance gene of human cancers.
AuthorsYamada T, Endo R, Gotoh M, Hirohashi S
JournalProc Natl Acad Sci U S A
PubMed ID9405678
'To improve cancer chemotherapy, a better understanding of the molecular mechanisms of drug resistance is essential. To identify the molecules responsible for drug resistance that is unrelated to MDR1 or MRP gene products, a eukaryotic expression cDNA library of cis-diamminedichloroplatinum(II) (CDDP)-resistant ovarian cancer TYKnuR cells was introduced into Cos-7 cells. ... More
Interaction of ethanol with inducers of glucose-regulated stress proteins. Ethanol potentiates inducers of grp78 transcription.
Authors Hsieh K P; Wilke N; Harris A; Miles M F;
JournalJ Biol Chem
PubMed ID8576245
GRP78, a molecular chaperone expressed in the endoplasmic reticulum, is a
AuthorsZhang X, Chattopadhyay A, Ji QS, Owen JD, Ruest PJ, Carpenter G, Hanks SK
JournalProc Natl Acad Sci U S A
PubMed ID10430888
The nonreceptor tyrosine kinase FAK (
Evidence that the WNT-inducible growth arrest-specific gene 1 encodes an antagonist of sonic hedgehog signaling in the somite.
Authors Lee C S; Buttitta L; Fan C M;
JournalProc Natl Acad Sci U S A
PubMed ID11572986
The dorsal-ventral polarity of the somite is controlled by antagonistic signals from the dorsal neural tube/surface ectoderm, mediated by WNTs, and from the ventral notochord, mediated by sonic hedgehog (SHH). Each factor can act over a distance greater than a somite diameter in vitro, suggesting they must limit each other's ... More
Stat3-Mediated Transformation of NIH-3T3 Cells by the Constitutively Active Q205L Go Protein
AuthorsRam PT, Horvath CM, Iyengar R
JournalScience
PubMed ID10615050
Expression of Q205L Galphao (Galphao*), an alpha subunit of heterotrimeric guanine nucleotide-binding proteins (G proteins) that lacks guanosine triphosphatase (GTPase) activity in NIH-3T3 cells, results in transformation. Expression of Galphao* in NIH-3T3 cells activated signal transducer and activator of transcription 3 (Stat3) but not mitogen-activated protein (MAP) kinases 1 or ... More
CARF is a novel protein that cooperates with mouse p19ARF (human p14ARF) in activating p53.
Authors Hasan Md Kamrul; Yaguchi Tomoko; Sugihara Takashi; Kumar Penmetcha K R; Taira Kazunari; Reddel Roger R; Kaul Sunil C; Wadhwa Renu;
JournalJ Biol Chem
PubMed ID12154087
The INK4a locus on chromosome 9p21 encodes two structurally distinct tumor suppressor proteins, p16(INK4a) and the alternative reading frame protein, ARF (p19(ARF) in mouse and p14(ARF) in human). Each of these proteins has a role in senescence of primary cells and activates pathways for cell cycle control and tumor suppression. ... More
Involvement of the small GTPase rho in integrin-mediated activation of mitogen-activated protein kinase.
Authors Renshaw M W; Toksoz D; Schwartz M A;
JournalJ Biol Chem
PubMed ID8702960
Engagement and clustering of integrins triggers a number of intracellular signaling events, including activation of the mitogen-activated protein (MAP) kinases Erk1 and Erk2. To investigate the mechanism by which integrins mediate the activation of MAP kinases upon binding of NIH 3T3 cells to fibronectin, we assessed the effects of both ... More
Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells.
Authors Li L; Cohen S N;
JournalCell
PubMed ID8616888
Using a novel strategy that enables the isolation of previously unknown genes encoding selectable recessive phenotypes, we identified a gene (tsg101) whose homozygous functional disruption produces cell transformation. Antisense RNA from a transactivated promoter introduced randomly into transcribed genes throughout the genome of mouse 3T3 fibroblasts was used to knock ... More
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
AuthorsSharma S, Murai F, Miyanohara A, Friedmann T
JournalProc Natl Acad Sci U S A
PubMed ID9380714
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse ... More
The adaptor protein Shc couples a class of integrins to the control of cell cycle progression.
Authors Wary K K; Mainiero F; Isakoff S J; Marcantonio E E; Giancotti F G;
JournalCell
PubMed ID8929541
We provide evidence that a class of integrins combines with the adaptor Shc and thereby with Grb2. Coimmunoprecipitation and mutagenesis experiments indicate that the recruitment of Shc is specified by the extracellular or transmembrane domain of integrin alpha subunit and suggest that this process is mediated by caveolin. Mutagenesis and ... More
The organellular chloride channel protein CLIC4/mtCLIC translocates to the nucleus in response to cellular stress and accelerates apoptosis.
AuthorsSuh KS, Mutoh M, Nagashima K, Fernandez-Salas E, Edwards LE, Hayes DD, Crutchley JM, Marin KG, Dumont RA, Levy JM, Cheng C, Garfield S, Yuspa SH,
JournalJ Biol Chem
PubMed ID14610078
CLIC4/mtCLIC, a chloride intracellular channel protein, localizes to the mitochondria and cytoplasm of keratinocytes and participates in the apoptotic response to stress. We now show that multiple stress inducers cause the translocation of cytoplasmic CLIC4 to the nucleus. Immunogold electron microscopy and confocal analyses indicate that nuclear CLIC4 is detected ... More
CrkL mediates Ras-dependent activation of the Raf/ERK pathway through the guanine nucleotide exchange factor C3G in hematopoietic cells stimulated with erythropoietin or interleukin-3.
AuthorsNosaka Y, Arai A, Miyasaka N, Miura O
JournalJ Biol Chem
PubMed ID10514505
CrkL is an SH2 and SH3 domain-containing adaptor protein implicated in pathogenesis of chronic myelogenous leukemia. Here, we demonstrate that overexpression of CrkL enhances the erythropoietin (Epo)- or interleukin (IL)-3-induced activation of Elk-1 and the c-fos gene promoter activity in 32D/EpoR-Wt cells. Moreover, the Epo-induced activation of ERK1 and ERK2 ... More
The baculovirus p35 protein inhibits Fas- and tumor necrosis factor- induced apoptosis.
AuthorsBeidler DR, Tewari M, Friesen PD, Poirier G, Dixit VM
JournalJ Biol Chem
PubMed ID7542648
The baculovirus p35 gene product inhibits virally induced apoptosis, developmental cell death in Caenorhabditis elegans and Drosophila, and neuronal cell death in mammalian systems. Therefore, p35 likely inhibits a component of the death machinery that is both ubiquitous and highly conserved in evolution. We now show for the first time ... More
Circadian Transcription. Thinking outside the E-Box.
AuthorsMuñoz E, Brewer M, Baler R,
JournalJ Biol Chem
PubMed ID12130638
The E-Box is a widely used DNA control element. Despite its brevity and broad distribution the E-Box is a remarkably versatile sequence that affects many different genetic programs, including proliferation, differentiation, tissue-specific responses, and cell death. The circadian clock is one of the latest pathways shown to employ this element. ... More
Perlecan is required to inhibit thrombosis after deep vascular injury and contributes to endothelial cell-mediated inhibition of intimal hyperplasia
AuthorsNugent MA, Nugent HM, Iozzo RV, Sanchack K, Edelman ER
JournalProc Natl Acad Sci U S A
PubMed ID10841569
Perlecan, a heparan sulfate proteoglycan, has been suggested to be critical for regulation of vascular repair. We generated clones of endothelial cells expressing an antisense vector targeting domain III of perlecan. Transfected cells produced significantly less perlecan than parent cells and showed a reduced ability to inhibit the binding and ... More
Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin.
P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like ligand for P- and E-selectin on human leukocytes. PSGL-1 requires sialylated, fucosylated O-linked glycans and tyrosine sulfate to bind P-selectin. Less is known about the determinants that PSGL-1 requires to bind E- selectin. To further define the modifications required for PSGL-1 to bind ... More
Transcriptional roles of nuclear factor kappa B and nuclear factor-interleukin-6 in the tumor necrosis factor alpha-dependent induction of cyclooxygenase-2 in MC3T3-E1 cells.
AuthorsYamamoto K, Arakawa T, Ueda N, Yamamoto S
JournalJ Biol Chem
PubMed ID8537402
When a mouse osteoblastic cell line MC3T3-E1 was cultured in the presence of tumor necrosis factor alpha (TNF alpha), the release of prostaglandin E2 and the cyclooxygenase activity increased in a dose- and time-dependent manner. The increase of the enzyme activity was attributed mostly to the induction of cyclooxygenase-2 rather ... More
Green fluorescent protein-based system for analysis of E-selectin- mediated adhesion.
Authorsel-Battari A, Zerfaoui M, Panicot L, Mas E, Prevot C, Lombardo D
JournalBiotechniques
PubMed ID10090997
Numerous cell-based or cell-free systems for study of selectin adhesion use radiolabeled tracers. However, in addition to handling problems associated with the use of radioisotopes, these assays have difficulty relating a number of counts to a number of adherent cells. Here, we describe an assay that uses the natural fluorescence ... More
Suppression of growth of renal carcinoma cells by the von Hippel-Lindau tumor suppressor gene.
Clear cell renal carcinomas are most frequently characterized by loss of function of both copies of the von Hippel-Lindau (VHL) disease gene, suggesting that the VHL gene product plays an important role in regulating renal cell proliferation. To directly assess the function of the VHL gene product, we transfected the ... More
Constitutively active NFkappa B is required for the survival of S-type neuroblastoma.
The NFkappaB transcription factors can both promote cell survival and induce apoptosis depending on cell type and context. Neuroblastoma (NB) cells display two predominant culture phenotypes identified as N- and S-types. Malignant S-type cells express neither high levels of MYCN nor Bcl-2, suggesting that other survival mechanisms are important. We ... More
Phosphorylation of critical serine residues in Gem separates cytoskeletal reorganization from down-regulation of calcium channel activity.
AuthorsWard Y, Spinelli B, Quon MJ, Chen H, Ikeda SR, Kelly K,
JournalMol Cell Biol
PubMed ID14701738
Gem is a small GTP-binding protein that has a ras-like core and extended chains at each terminus. The primary structure of Gem and other RGK family members (Rad, Rem, and Rem2) predicts a GTPase deficiency, leading to the question of how Gem functional activity is regulated. Two functions for Gem ... More
Retrovirus-derived vectors are overwhelmingly preferred over other methods for ex vivo gene therapy because they provide permanent integration of foreign genes into cellular DNA. In comparison, cationic lipids mediate efficent gene transfer, but expression is transient. When we combined cationic lipids with retrovirus particles we obtained a significant enhancement of ... More
Efficient intracellular assembly of papillomaviral vectors.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More
Growth inhibition by keratinocyte growth factor receptor of human salivary adenocarcinoma cells through induction of differentiation and apoptosis.
Authors Zhang Y; Wang H; Toratani S; Sato J D; Kan M; McKeehan W L; Okamoto T;
JournalProc Natl Acad Sci U S A
PubMed ID11562460
We have reported that normal human salivary gland-derived epithelial cells exclusively express keratinocyte growth factor receptor (KGFR). In the process of malignant transformation of human salivary gland tumors, KGFR gene expression disappeared concomitantly with the de novo expression of the fibroblast growth factor receptor 1 (FGFR1) and FGFR4 genes. In ... More
Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling.
Mastermind (Mam) is one of the evolutionarily conserved elements of Notch signaling. Genetic analyses in Drosophila implicated it as an important positive regulator of the pathway. We show here identification of two new members of human Mam family (human Mastermind-2 (hMam-2) and human Mastermind-3 (hMam-3)), which retain characteristics similar to ... More
Polypyrimidine tract-binding proteins are cleaved by caspase-3 during apoptosis.
Authors Back Sung Hoon; Shin Sejeong; Jang Sung Key;
JournalJ Biol Chem
PubMed ID12004072
The polypyrimidine tract-binding protein (PTB), an RNA-binding protein, is required for efficient translation of some mRNAs containing internal ribosomal entry sites (IRESs). Here we provide evidence that the addition of apoptosis-inducing agents to cells results in the cleavage of PTB isoforms 1, 2, and 4 by caspase-3. This cleavage of ... More
Cyclin D1 Is a Ligand-independent Co-repressor for Thyroid Hormone Receptors.
Authors Lin Huei-Min; Zhao Li; Cheng Sheue-Yann;
JournalJ Biol Chem
PubMed ID12048199
Thyroid hormone receptors (TRs) are critical regulators of growth, differentiation, and homeostasis. TRs function by regulating the expression of thyroid hormone (T3) target genes in both ligand-dependent and -independent pathways. Distinct classes of co-regulatory proteins modulate these two pathways. We show here a novel role of cyclin D1 as a ... More
Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons.
Authors Pedarzani Paola; D'hoedt Dieter; Doorty Kevina B; Wadsworth Jonathan D F; Joseph Jeremiah S; Jeyaseelan Kandiah; Kini R Manjunatha; Gadre S V; Sapatnekar S M; Stocker Martin; Strong Peter N;
JournalJ Biol Chem
PubMed ID12239213
The biophysical properties of small conductance Ca(2+)-activated K(+) (SK) channels are well suited to underlie afterhyperpolarizations (AHPs) shaping the firing patterns of a conspicuous number of central and peripheral neurons. We have identified a new scorpion toxin (tamapin) that binds to SK channels with high affinity and inhibits SK channel-mediated ... More
Mediation of the DCC apoptotic signal by DIP13 alpha.
Authors Liu Jiayou; Yao Fayi; Wu Ruping; Morgan Michael; Thorburn Andrew; Finley Russell L Jr; Chen Yong Q;
JournalJ Biol Chem
PubMed ID12011067
DCC (deleted in colorectal cancer) is a candidate tumor suppressor gene. However the function of DCC remains elusive. Previously, we demonstrated that forced expression of DCC induces apoptosis or cell cycle arrest (Chen, Y. Q., Hsieh, J. T., Yao, F., Fang, B., Pong, R. C., Cipriano, S. C. & Krepulat, ... More
c-Myc sensitizes cells to tumor necrosis factor-mediated apoptosis by inhibiting nuclear factor kappa B transactivation.
Authors You Zongbing; Madrid Lee V; Saims Daniel; Sedivy John; Wang Cun-Yu;
JournalJ Biol Chem
PubMed ID12149248
Nuclear factor kappaB (NF-kappaB) plays a key role in suppression of tumor necrosis factor (TNF)-mediated apoptosis by inducing a variety of anti-apoptotic genes. Expression of c-Myc has been shown to sensitize cells to TNF-mediated apoptosis by inhibiting NF-kappaB activation. However, the precise step in the NF-kappaB signaling pathway and apoptosis ... More
Ras mediates the activation of phospholipase D by v-Src.
Authors Jiang H; Lu Z; Luo J Q; Wolfman A; Foster D A;
JournalJ Biol Chem
PubMed ID7890731
We demonstrated previously that v-Src activates a phospholipase D (PLD) activity (Song, J., Pfeffer, L.M., and Foster, D.A. (1991) Mol. Cell. Biol. 11, 4903-4908) and that this activation is dependent upon a G protein(s) (Jiang H., Alexandropoulos, K., Song, J., and Foster, D.A. (1994) Mol. Cell. Biol. 14, 3676-3682). An ... More
Expression of transforming growth factor type III receptor in vascular endothelial cells increases their responsiveness to transforming growth factor beta 2.
Authors Sankar S; Mahooti-Brooks N; Centrella M; McCarthy T L; Madri J A;
JournalJ Biol Chem
PubMed ID7768960
Bovine aortic endothelial cells (BAECs) express both type I and type II receptors for transforming growth factor beta (TGF beta). These cells respond to TGF beta 1 but are relatively refractory to another isoform of TGF beta, termed TGF beta 2. TGF beta s are thought to signal through receptor ... More
Mammalian Suppressor of Sec4 Modulates the Inhibitory Effect of Rab15 during Early Endocytosis.
Authors Strick David J; Francescutti Dina M; Zhao Yali; Elferink Lisa A;
JournalJ Biol Chem
PubMed ID12105226
Rab15 is a novel endocytic Rab that counters the stimulatory effect of Rab5-GTP on early endocytic trafficking. Rab15 may interfere with Rab5 function directly by sequestering Rab5 effectors or indirectly through novel sets of effector interactions. To distinguish between these possibilities, we examined the effector binding properties of Rab15. Rab15 ... More
PDZ-domain interactions and apical expression of type IIa Na/P(i) cotransporters.
Type IIa Na/P(i) cotransporters are expressed in renal proximal brush border and are the major determinants of inorganic phosphate (P(i)) reabsorption. Their carboxyl-terminal tail contains information for apical expression, and interacts by means of its three terminal amino acids with several PSD95/DglA/ZO-1-like domain (PDZ)-containing proteins. Two of these proteins, NaPi-Cap1 ... More
A short segment of the R domain of cystic fibrosis transmembrane conductance regulator contains channel stimulatory and inhibitory activities that are separable by sequence modification.
Authors Xie Junxia; Adams Lynn M; Zhao Jiying; Gerken Thomas A; Davis Pamela B; Ma Jianjie;
JournalJ Biol Chem
PubMed ID11950844
The regulatory (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) contains consensus phosphorylation sites for cAMP-dependent protein kinase (PKA) that are the basis for physiological regulation of the CFTR chloride channel. A short peptide segment in the R domain with a net negative charge of B9 (amino acids ... More
The first proline of PALP motif at the C terminus of presenilins is obligatory for stabilization, complex formation, and gamma-secretase activities of presenilins.
Mutations in presenilin (PS) genes cause early-onset familial Alzheimer's disease by increasing production of the amyloidogenic form of amyloid beta peptides ending at residue 42 (Abeta42). PS is an evolutionarily conserved multipass transmembrane protein, and all known PS proteins contain a proline-alanine-leucine-proline (PALP) motif starting at proline (P) 414 (amino ... More
Targeted gap junction protein constructs reveal connexin-specific differences in oligomerization.
Authors Sarma Jayasri Das; Wang Fushan; Koval Michael;
JournalJ Biol Chem
PubMed ID11929864
To define further the mechanisms of gap junction protein (connexin (Cx)) oligomerization without pharmacologic disruption, we have examined the transport and assembly of connexin constructs containing C-terminal di-lysine-based endoplasmic reticulum (ER) (HKKSL) or ER-Golgi intermediate compartment (AKKFF) targeting sequences. By immunofluorescence microscopy, Cx43-HKKSL transiently transfected into HeLa cells showed a ... More
Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology.
A new member of the dynamin GTPase family (OPA1) was recently identified in humans and shown to be mutated in patients with dominant optic atrophy. To understand better the function of mammalian OPA1, we isolated a mouse ortholog (mOPA1) from brain and raised a specific antibody against its C terminus. ... More
Endocytosis of Epidermal Growth Factor Receptor Regulated by Grb2-mediated Recruitment of the Rab5 GTPase-activating Protein RN-tre.
Authors Martinu Lenka; Santiago-Walker Ademi; Qi Hongwei; Chou Margaret M;
JournalJ Biol Chem
PubMed ID12399475
The Grb2 adaptor protein is best known for its role in signaling to the small GTPase p21(ras), mediated through its interaction with the SOS guanine nucleotide exchange factor. Here, we demonstrate that Grb2 also signals to Rab5, a small GTPase that plays a key role in early endocytic trafficking. Grb2 ... More
Three adjacent serines in the extracellular domains of the CaR are required for L-amino acid-mediated potentiation of receptor function.
Authors Zhang Zaixiang; Qiu Weiying; Quinn Stephen J; Conigrave Arthur D; Brown Edward M; Bai Mei;
JournalJ Biol Chem
PubMed ID12095982
The extracellular calcium (Ca(2+)(o))-sensing receptor (CaR) is a key player in Ca(2+)(o) homeostasis. The activity of CaR can be potentiated by various l-amino acids. In this study, we examined whether conserved amino acid residues involved in the binding of glutamate to metabotropic glutamate receptors (mGluRs) also participate in the potentiation ... More
A selective interaction between OS-9 and the carboxyl-terminal tail of meprin beta.
OS-9, a protein previously uncharacterized, was shown to interact specifically with the intracellular region of the membrane proteinase meprin beta found in brush border membranes of kidney and small intestine. We have shown previously that this cytoplasmic region is indispensable for the maturation of meprin beta, which included an endoplasmic ... More
Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors.
Addition of ubiquitin or ubiquitin chains to target proteins leads to their mono- or polyubiquitination, respectively. Whereas polyubiquitination targets proteins for degradation, monoubiquitination is thought to regulate receptor internalization and endosomal sorting. Cbl proteins are major ubiquitin ligases that promote ligand-dependent polyubiquitination and degradation of receptor tyrosine kinases. They also ... More
Two novel transcripts for human endothelin B receptor produced by RNA editing/alternative splicing from a single gene.
Hirschsprung disease is a heterogeneous genetic disorder, causative genes of which include the endothelin B receptor (ETB). To investigate the mutations of ETB in Hirschsprung disease, expression of the ETB gene in lymphoblastoid cells from patients and normal healthy adults was examined, and novel mutant transcripts were found. The mutant ... More
Role of N-linked oligosaccharide flexibility in mannose phosphorylation of lysosomal enzyme cathepsin L.
Authors Warner Jason B; Thalhauser Craig; Tao Kai; Sahagian G Gary;
JournalJ Biol Chem
PubMed ID12202476
Mannose phosphorylation of N-linked oligosaccharides by UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase is a key step in the targeting of lysosomal enzymes in mammalian cells and tissues. The selectivity of this process is determined by lysine-based phosphorylation signals shared by lysosomal enzymes of diverse structure and function. By introducing new glycosylation sites at ... More
The ryanodine receptor modulates the spontaneous beating rate of cardiomyocytes during development.
Authors Yang Huang-Tian; Tweedie David; Wang Su; Guia Antonio; Vinogradova Tatiana; Bogdanov Konstantin; Allen Paul D; Stern Michael D; Lakatta Edward G; Boheler Kenneth R;
JournalProc Natl Acad Sci U S A
PubMed ID12089338
In adult myocardium, the heartbeat originates from the sequential activation of ionic currents in pacemaker cells of the sinoatrial node. Ca(2+) release via the ryanodine receptor (RyR) modulates the rate at which these cells beat. In contrast, the mechanisms that regulate heart rate during early cardiac development are poorly understood. ... More
Human T-cell leukemia virus type I Tax protein transactivates RNA polymerase III promoter in vitro and in vivo.
Tax protein of the human T-cell lymphotropic virus type 1 (HTLV-I) is critical for viral replication and is a potent transcriptional activator of viral and cellular polymerase II (pol II) genes. We report here that Tax is able to transactivate a classical pol III promoter, VA-I. In cotransfection experiments, Tax ... More
Regulation of angiotensin II-induced G protein signaling by phosducin-like protein.
Authors McLaughlin Joseph N; Thulin Craig D; Bray Steven M; Martin Mickey M; Elton Terry S; Willardson Barry M;
JournalJ Biol Chem
PubMed ID12107186
Phosducin-like protein (PhLP) is a broadly expressed member of the phosducin (Pd) family of G protein betagamma subunit (Gbetagamma)-binding proteins. Though PhLP has been shown to bind Gbetagamma in vitro, little is known about its physiological function. In the present study, the effect of PhLP on angiotensin II (Ang II) ... More
Transcriptional analysis of the 5'-noncoding region of the human involucrin gene.
Authors Lopez-Bayghen E; Vega A; Cadena A; Granados S E; Jave L F; Gariglio P; Alvarez-Salas L M;
JournalJ Biol Chem
PubMed ID8550612
Human involucrin whose gene transcription is directed by a 2456-nucleotide (nt) 5'-noncoding region is a structural component of the epithelial cornified layer. Transient transfection assays demonstrated that this region is transcriptionally active in multiplying keratinocytes and is enhanced by 2 mM CaCl2 treatment. Calcium-independent transcriptional activity and the interaction with ... More
Peg3/Pw1 is involved in p53-mediated cell death pathway in brain ischemia/hypoxia.
Emerging evidence has shown that tumor suppressor p53 expression is enhanced in response to brain ischemia/hypoxia and that p53 plays a critical role in the cell death pathway in such an acute neurological insult. However the mechanism remains unclear. Recently it was reported that Peg3/Pw1, originally identified as a paternally ... More
Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis.
Authors Huang Chi-Ying F; Wu Yi-Mi; Hsu Chiung-Yueh; Lee Wan-Shu; Lai Ming-Derg; Lu Te-Jung; Huang Chia-Lin; Leu Tzeng-Horng; Shih Hsiu-Ming; Fang Hsin-I; Robinson Dan R; Kung Hsing-Jien; Yuan Chiun-Jye;
JournalJ Biol Chem
PubMed ID12107159
Mammalian Sterile 20-like kinase 3 (Mst3), the physiological functions of which are unknown, is a member of the germinal center kinase-III family. It contains a conserved kinase domain at its NH(2) terminus, whereas there is a regulatory domain at its COOH terminus. In this study we demonstrate that endogenous Mst3 ... More