Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA.
AuthorsFlatz L,Bergthaler A,de la Torre JC,Pinschewer DD
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID16537369
The prototypic arenavirus lymphocytic choriomeningitis virus has been a primary workhorse of viral immunologists for almost a century, and it has served as an important model for studying basic principles of arenavirus molecular biology. Its negative-stranded bisegmented RNA genome has, however, posed a major obstacle to attempts at manipulating the ... More
Reconstitution of human DNA polymerase delta using recombinant baculoviruses: the p12 subunit potentiates DNA polymerizing activity of the four-subunit enzyme.
Authors Podust Vladimir N; Chang Long-Sheng; Ott Robert; Dianov Grigory L; Fanning Ellen;
JournalJ Biol Chem
PubMed ID11711545
'Eukaryotic DNA polymerase delta is thought to consist of three (budding yeast) or four subunits (fission yeast, mammals). Four human genes encoding polypeptides p125, p50, p66, and p12 have been assigned as subunits of DNA polymerase delta. However, rigorous purification of human or bovine DNA polymerase delta from natural sources ... More
The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing.
Authors Que Xuchu; Ngo Huân; Lawton Jeffrey; Gray Mary; Liu Qing; Engel Juan; Brinen Linda; Ghosh Partho; Joiner Keith A; Reed Sharon L;
JournalJ Biol Chem
PubMed ID12000756
'Cysteine proteinases play a major role in invasion and intracellular survival of a number of pathogenic parasites. We cloned a single copy gene, tgcp1, from Toxoplasma gondii and refolded recombinant enzyme to yield active proteinase. Substrate specificity of the enzyme and homology modeling identified the proteinase as a cathepsin B. ... More
Misexpression of the eyes absent family triggers the apoptotic program.
Authors Clark S Wesley; Fee Brian E; Cleveland John L;
JournalJ Biol Chem
PubMed ID11700312
'Genetic studies in Drosophila and mice have shown that eyes absent (eya) is an important and conserved transcriptional regulator of development. Along with eyeless/Pax6, sine oculis, and dachshund, eya genes function as master regulators in eye development and can induce ectopic eye formation. Furthermore, the loss-of-function mutants of these genes ... More
Characterization of three alternatively spliced isoforms of the Rel/NF-kappa B transcription factor Relish from the mosquito Aedes aegypti.
Authors Shin Sang Woon; Kokoza Vladimir; Ahmed Abduelaziz; Raikhel Alexander S;
JournalProc Natl Acad Sci U S A
PubMed ID12119421
'The Rel/NF-kappa B transcription factor Relish performs a central role in the acute-phase response to microbial challenge by activating immune antibacterial peptides. We cloned and molecularly characterized the gene homologous to Drosophila Relish from the mosquito Aedes aegypti. Unlike Drosophila Relish, Aedes Relish has three alternatively spliced transcripts encoding different ... More
Molecular characterization of the starfish inositol 1,4,5-trisphosphate receptor and its role during oocyte maturation and fertilization.
The release of calcium ions (Ca(2+)) from their intracellular stores is essential for the fertilization of oocytes of various species. The calcium pools can be induced to release Ca(2+) via two main types of calcium channel receptor: the inositol 1,4,5-trisphosphate receptor (IP(3)R) and the ryanodine receptor. Starfish oocytes have often ... More
The Caenorhabditis elegans gene, gly-2, can rescue the N-acetylglucosaminyltransferase V mutation of Lec4 cells.
Authors Warren Charles E; Krizus Aldis; Roy Peter J; Culotti Joseph G; Dennis James W;
JournalJ Biol Chem
PubMed ID11937505
UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V (GlcNAc-TV) is a regulator of polylactosamine-containing N-glycans and is causally involved in T cell regulation and tumor metastasis. The Caenorhabditis elegans genome contains a single orthologous gene, gly-2, that is transcribed and encodes a 669-residue type II membrane protein that is 36.7% identical to mammalian GlcNAc-TV (Mgat-5). ... More
Functional Expression and Characterization of Macaque C-C Chemokine Receptor 3 (CCR3) and Generation of Potent Antagonistic Anti-macaque CCR3 Monoclonal Antibodies.
Authors Zhang Liwen; Soares Marco P; Guan Yanfen; Matheravidathu Stephen; Wnek Richard; Johnson Kristine E; Meisher Anna; Iliff Susan A; Mudgett John S; Springer Martin S; Daugherty Bruce L;
JournalJ Biol Chem
PubMed ID12101185
Eosinophils are major effector cells implicated in a number of chronic inflammatory diseases in humans, particularly bronchial asthma and allergic rhinitis. The beta-chemokine receptor C-C chemokine receptor 3 (CCR3) provides a mechanism for the selective recruitment of eosinophils into tissue and thus has recently become an attractive biological target for ... More