dNTP 混合物(每种 10 mM)
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dNTP 混合物(每种 10 mM)
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dNTP 混合物(每种 10 mM)

Invitrogen 10 mM dNTP 混合物是溶于 0.6 mM Tris-HCl 中的四种核苷酸 (dATP,了解更多信息
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货号数量
18427013100 μL
184270895 x 1 mL
184270881 mL
货号 18427013
价格(CNY)
672.00
飞享价
Ends: 31-Dec-2025
978.00
共减 306.00 (31%)
Each
添加至购物车
数量:
100 μL
请求批量或定制报价
价格(CNY)
672.00
飞享价
Ends: 31-Dec-2025
978.00
共减 306.00 (31%)
Each
添加至购物车

Invitrogen 10 mM dNTP 混合物是溶于 0.6 mM Tris-HCl 中的四种核苷酸 (dATP, dCTP, dGTP, dTTP) 的混合物。每种核苷酸浓度均为 10 mM。10 mM dNTP 混合物适用于聚合酶链式反应 (PCR)、测序、填充反应、切口平移、cDNA 合成和 TdT 加尾反应。

Invitrogen dNTP 的生产符合极高行业标准:
•按照 ISO 9001 进行生产
• 在 D 级清洁室中进行生产

特点包括:
•化学合成
• pH 值 7.5
• 通过 HPLC 确认纯度 > 99%
• 在 –20°C 下可稳定储存 2 年
• 无 qPCR、PCR、逆转录抑制剂
• 无 DNase 和 RNase
• 无人和大肠杆菌 DNA

应用:
• qPCR、RT-qPCR
• PCR、RT-PCR、cDNA 合成
• 高保真和长片段 PCR
• 等温扩增
• DNA 标记
• 克隆
• Sanger 和下一代测序 (NGS) 测序

仅供科研使用。不可用于诊断程序。
规格
数量100 μL
运输条件经批准可置于湿冰或干冰上运输
最大浓度40 mM
适用于(应用)Standard PCR, RT-PCR, Real Time PCR (qPCR)
形式液体
标签或染料未标记
Unit SizeEach
内容与储存
1 个 100 µL 样品瓶

混合物含有全部四种 dNTP,每种 dNTP 在 Tris-HCl(pH 值 7.5)中的最终浓度均为 10 mM。储存(在 -20°C 下)。

常见问题解答 (FAQ)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions

Why does the annealing temperature have to be above 55 degrees C, when using the GeneAmp PCR Carryover Prevention kit (Cat. No. N808-0068) or any kit containing AmpErase UNG (Cat. No. N808-0096)?

Uracil-N-glycosylase (UNG) has activity in the temperature range of 4 degrees C to 55 degrees C, and is not completely inactivated by incubation at 95 degrees C. It is recommended that the annealing temperature be at or above 55 degrees C when using this enzyme with any of our PCR or Sequence Detection Systems kits to avoid possible degradation of newly synthesized dU-containing PCR products by residual UNG activity. For this reason, it is also recommended that the post-PCR soak be maintained at 72 degrees C (instead of 4 degrees C) if analysis of the PCR product will be performed using a gel-based method.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Describe the amplified product produced by the control reagents in the GeneAmp Gold PCR Reagent Kit with Multiplex Control (Cat. No. 4312778).

The control primers generate a 300 and a 500 bp amplicon from the lambda control DNA. The Lambda Multiplex Control primers, as well as the Lambda Control DNA are provided in this kit.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.