Metabolic exploitation of the sialic acid biosynthetic pathway to generate site-specifically labeled antibodies.
AuthorsRochefort MM, Girgis MD, Ankeny JS, Tomlinson JS,
Journal
PubMed ID24150277
Lack of a universal site-specific conjugation methodology for antibodies limits their potential to be developed as tumor-specific imaging agents or targeted therapeutics. A potential mechanism for site-specific conjugation involves utilization of the conserved N-glycosylation site in the CH2 domain. We sought to develop an antibody with an altered azido-sugar at ... More