Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.
AuthorsGebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L
JournalNucleic Acids Res
PubMed ID3588302
'A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine and the 4-position of cytosine. These nucleotides are incorporated ... More
Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.
AuthorsFlickinger JL, Gebeyehu G, Buchman G, Haces A, Rashtchian A,
JournalNucleic Acids Res
PubMed ID1317552
Comparison of detection and signal amplification methods for DNA microarrays.
AuthorsVora GJ, Meador CE, Anderson GP, Taitt CR,
JournalMol Cell Probes
PubMed ID18675897
One of the factors limiting the use of DNA microarray technology for the detection of pathogenic organisms from clinical and environmental matrices has been inadequate assay sensitivity. To assess the effectiveness of post-hybridization secondary detection steps to enhance the sensitivity of DNA microarray-based pathogen detection, we evaluated a panel of ... More