tRNA for LightShift™ Chemiluminescent RNA EMSA Kit - FAQs

What is an electrophoretic mobility shift assay (EMSA)?

EMSAs (also called gel shifts, band shifts, gel retardation assays, or mobility assays) have been used extensively for studying protein-DNA interactions. Because protein-DNA complexes migrate more slowly through a native polyacrylamide or agarose gel than DNA alone, individual protein-DNA complexes can be visualized as discrete bands within the gel using chemiluminescence or radioisotopic detection.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.