Pierce™ DTT（二硫苏糖醇）

Name: Pierce™ DTT（二硫苏糖醇）
SKU: 20290
Price: 2722.00 CNY

二硫苏糖醇是多种蛋白研究应用常用的还原剂，包括还原聚丙烯酰胺电泳 (SDS-PAGE)。相关产品Bond-Breaker™ TCEP 溶液，中性 pH 值了解更多信息

货号 20290

价格（CNY)

2,722.00

Each

添加至购物车

请求批量或定制报价

价格（CNY)

2,722.00

Each

添加至购物车

二硫苏糖醇是多种蛋白研究应用常用的还原剂，包括还原聚丙烯酰胺电泳 (SDS-PAGE)。

相关产品
Bond-Breaker™ TCEP 溶液，中性 pH 值

For Research Use Only. Not for use in diagnostic procedures.

规格

描述Pierce DTT，Cleland 试剂

形式粉末

产品线Pierce™

数量5 g

产品类型二硫苏糖醇

Unit SizeEach

常见问题解答 (FAQ)

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.