Pierce™ High Capacity Streptavidin Agarose, 10 mL - FAQs

查看更多产品信息 Pierce™ High Capacity Streptavidin Agarose - FAQs (20357, 20361, 20359)

2 个常见问题解答

How many biotin binding-sites are there per streptavidin molecule?

Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the binding capacity of the High Capacity Streptavidin Agarose?

The High Capacity Streptavidin Agarose resin has a binding capacity of >100 µg biotinylated p-NPE/mL of settled resin and >10 mg biotinylated BSA/mL of settled resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.