Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs.
AuthorsAlian A, Griner SL, Chiang V, Tsiang M, Jones G, Birkus G, Geleziunas R, Leavitt AD, Stroud RM
JournalProc Natl Acad Sci U S A
PubMed ID19416821
HIV-1 integration into the host cell genome is a multistep process catalyzed by the virally-encoded integrase (IN) protein. In view of the difficulty of obtaining a stable DNA-bound IN at high concentration as required for structure determination, we selected IN-DNA complexes that form disulfide linkages between 5'-thiolated DNA and several ... More
Structural and molecular basis of the assembly of the TRPP2/PKD1 complex.
AuthorsYu Y, Ulbrich MH, Li MH, Buraei Z, Chen XZ, Ong AC, Tong L, Isacoff EY, Yang J
JournalProc Natl Acad Sci U S A
PubMed ID19556541
Mutations in PKD1 and TRPP2 account for nearly all cases of autosomal dominant polycystic kidney disease (ADPKD). These 2 proteins form a receptor/ion channel complex on the cell surface. Using a combination of biochemistry, crystallography, and a single-molecule method to determine the subunit composition of proteins in the plasma membrane ... More
Enhanced tissue penetration-induced high bonding strength of a novel tissue adhesive composed of cholesteryl group-modified gelatin and disuccinimidyl tartarate.
AuthorsMatsuda M, Ueno M, Endo Y, Inoue M, Sasaki M, Taguchi T
JournalColloids Surf B Biointerfaces
PubMed ID22088758
The aim of this study was to evaluate the effect of cholesteryl group content on the bonding strength of a novel tissue adhesive composed of cholesteryl group-modified geletin (CholGltn) and disuccinimidyl tartarate (DST). The bonding strength of this tissue adhesive with fresh arterial media reached a maximum at a CholGltn ... More
Arabidopsis Cor15am is a chloroplast stromal protein that has cryoprotective activity and forms oligomers.
Many plants acquire increased freezing tolerance when they are exposed to nonfreezing temperatures of a certain duration. This process is known as cold acclimation and allows plants to protect themselves from freezing injury. A wide variety of polypeptides are induced during cold acclimation, among which is one encoded by COR15A ... More