Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries.
AuthorsGuergova-Kuras M, Kurucz I, Hempel W, Tardieu N, Kádas J, Malderez-Bloes C, Jullien A, Kieffer Y, Hincapie M, Guttman A, Csánky E, Dezso B, Karger BL, Takács L
JournalMol Cell Proteomics
PubMed ID21947365
'A challenge in the treatment of lung cancer is the lack of early diagnostics. Here, we describe the application of monoclonal antibody proteomics for discovery of a panel of biomarkers for early detection (stage I) of non-small cell lung cancer (NSCLC). We produced large monoclonal antibody libraries directed against the ... More
The structural characterization of a prophage-encoded extracellular DNase from Streptococcus pyogenes.
AuthorsKorczynska JE, Turkenburg JP, Taylor EJ
JournalNucleic Acids Res
PubMed ID21948797
'The pathogenic bacterium Group A Streptococcus pyogenes produces several extracellular DNases that have been shown to facilitate invasive infection by evading the human host immune system. DNases degrade the chromatin in neutrophil extracellular traps, enabling the bacterium to evade neutrophil capture. Spd1 is a type I, nonspecific ßßa/metal-dependent nuclease from ... More
Outer membrane lipoprotein Lpp is Gram-negative bacterial cell surface receptor for cationic antimicrobial peptides.
AuthorsChang TW, Lin YM, Wang CF, Liao YD
JournalJ Biol Chem
PubMed ID22084237
Antimicrobial peptides/proteins (AMPs) are important components of the host innate defense mechanisms. Here we demonstrate that the outer membrane lipoprotein, Lpp, of Enterobacteriaceae interacts with and promotes susceptibility to the bactericidal activities of AMPs. The oligomeric Lpp was specifically recognized by several cationic a-helical AMPs, including SMAP-29, CAP-18, and LL-37; ... More
Inhibition of RNase L and RNA-dependent protein kinase (PKR) by sunitinib impairs antiviral innate immunity.
AuthorsJha BK, Polyakova I, Kessler P, Dong B, Dickerman B, Sen GC, Silverman RH
JournalJ Biol Chem
PubMed ID21636578
RNase L and RNA-dependent protein kinase (PKR) are effectors of the interferon antiviral response that share homology in their pseudokinase and protein kinase domains, respectively. Sunitinib is an orally available, ATP-competitive inhibitor of VEGF and PDGF receptors used clinically to suppress angiogenesis and tumor growth. Sunitinib also impacts IRE1, an ... More
Human mitochondrial DNA helicase TWINKLE is both an unwinding and annealing helicase.
AuthorsSen D, Nandakumar D, Tang GQ, Patel SS
JournalJ Biol Chem
PubMed ID22383523
TWINKLE is a nucleus-encoded human mitochondrial (mt)DNA helicase. Point mutations in TWINKLE are associated with heritable neuromuscular diseases characterized by deletions in the mtDNA. To understand the biochemical basis of these diseases, it is important to define the roles of TWINKLE in mtDNA metabolism by studying its enzymatic activities. To ... More