Pierce™ 温和抗原/抗体洗脱缓冲液，pH 6.6
Pierce™ 温和抗原/抗体洗脱缓冲液，pH 6.6
Thermo Scientific™

Pierce™ 温和抗原/抗体洗脱缓冲液，pH 6.6

Thermo Scientific Pierce IgG 结合和洗脱缓冲液采用蛋白 A 和蛋白 G 琼脂糖微珠等 IgG了解更多信息
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货号数量
210133.75 L
21027500 mL
货号 21013
价格（CNY)
6,345.00
Each
添加至购物车
数量:
3.75 L
请求批量或定制报价
价格（CNY)
6,345.00
Each
添加至购物车
Thermo Scientific Pierce IgG 结合和洗脱缓冲液采用蛋白 A 和蛋白 G 琼脂糖微珠等 IgG 亲和纯化支持物，实现高得率、非变性抗体纯化。Gentle 抗原/抗体的结合和洗脱缓冲体系在近中性的高盐条件下洗脱，即使是敏感和不稳定的抗体（或其他蛋白质）也能够从亲和体系中安全洗脱，不会发生变性和失活。该系统中的无磷酸盐结合缓冲液是蛋白 A 结合缓冲液（pH 值 8）的必要替代品，后者可与温和抗原/抗体洗脱缓冲液形成沉淀而析出。

所有 Thermo Scientific Pierce IgG 结合和洗脱缓冲液的共同特点：

•即用型—精心配制和预滤的 1X 溶液
• 三种结合缓冲液—针对蛋白 A、蛋白 G 或者蛋白 A/G 进行了优化
• 两种洗脱缓冲液可供选择—酸性（pH 值 2.8）或高盐（近中性）
• 通用—洗脱缓冲液适用于抗体-抗原亲和纯化

提供适用于各种抗体纯化方法的产品：
•蛋白 A IgG 结合缓冲液：pH 值为 8.0；基于磷酸盐；包含甘油和 EDTA
•蛋白 A/G IgG 结合缓冲液：pH 值为 8.0；基于磷酸盐；包含 EDTA
•蛋白 G IgG 结合缓冲液：pH 值为 5.0；包含 0.02% 叠氮化钠
•IgG 洗脱缓冲液：pH 值为 2.8；基于胺
•温和 Ag/Ab 结合缓冲液：pH 值为 8.0；无磷酸盐；包含 0.05% 叠氮化钠
•温和 Ag/Ab 洗脱缓冲液：pH 值为 6.6；高盐（离子强度）

相关产品
Pierce™ IgG 洗脱缓冲液
Pierce™ 温和 Ag/Ab 结合缓冲液，pH 值为 8.0
Pierce™ 温和 Ag/Ab 结合和洗脱缓冲液试剂盒
仅供科研使用。不可用于诊断程序。
规格
化学名称或材料洗脱缓冲液
建议的储存条件4°C
物理形态液体
产品线Pierce™
数量3.75 L
pH6.6
Unit SizeEach

常见问题解答 (FAQ)

After immobilizing my antibody, the first affinity purification of my protein worked well, but the protein failed to bind to the antibody during the second round of purification. What did I do wrong?

Low pH elution is the most commonly used method of elution for affinity purification, however, it could lead to denaturation of some antibodies which will negatively affect subsequent antigen binding. To prevent this from happening, after protein purification and column regeneration, immediately wash the column with neutralization or binding buffer and store at 4 degrees C in buffer containing an antimicrobial agent such as sodium azide.

Alternatively, use a near-neutral, high salt elution buffer such as Pierce Gentle Ag/Ab Elution Buffer (Cat. No. 21013, 21027, or 21030). For alternative elution buffers, please refer to this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0027-Elution-conditions.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.