Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit - FAQs

How many purifications can be performed using the same affinity column?

This varies with the stability of the immobilized protein and the type of elution buffer used. Columns can typically be reused at least 10 times without significant loss of activity. If the immobilized molecule is sensitive to low pH, consider using our Pierce Gentle Ag/Ab Elution Buffer (Cat. No. 21013, 21027 or 21030). For alternative elution buffers, please refer to this Tech Tip(http://tools.thermofisher.com/content/sfs/brochures/TR0027-Elution-conditions.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.