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Pierce™ Western Blot Signal Enhancer - FAQs

查看更多产品信息 Pierce™ Western Blot Signal Enhancer - FAQs (21050)

15 个常见问题解答

Western Blot信号增强剂会干扰蛋白的检测吗？

Western Blot信号增强剂不会和膜或蛋白质结合，因此不会干扰抗体和目的蛋白的结合。

我能在剥离后的膜上使用Western Blot信号增强剂吗？

剥离后的膜上仍有封闭试剂存留，所以你会得到不一致的结果。因此，我们建议仅在封闭和第一次膜杂交前使用Western Blot信号增强剂。

我能在使用Western Blot信号增强剂前用丽春红染膜吗？

我们推荐在染膜和封闭前使用Western Blot信号增强剂。

我需要一种WB底物和Western Blot信号增强剂一起使用吗？

Western Blot信号增强剂可以对任何发光或显色底物起作用。

Western Blot信号增强剂是如何使信号增强的？

Western Blot信号增强剂可以松弛蛋白，以便抗体和蛋白能更有效的结合。

什么时候应该使用Western Blot信号增强剂?

Western Blot信号增强剂应在将蛋白质转膜后，封闭或染膜前使用。

How can I increase detection sensitivity with SuperSignal West Femto Maximum Sensitivity Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase detection sensitivity with SuperSignal West Dura Extended Duration Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other?

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will the Pierce Western Blot Signal Enhancer interfere with protein detection?

The Enhancer does not bind to the membrane or proteins so it should not interfere with an antibody in binding the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the Pierce Western Blot Signal Enhancer on a stripped blot?

After stripping the blot, blocking reagent remains on the membrane so you will get inconsistent results. Therefore, we recommend using the Enhancer only before blocking and the first probing of the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I stain my membrane with Ponceau S prior to using the Pierce Western Blot Signal Enhancer?

We recommend using the Enhancer prior to staining and blocking.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do I need to use a Pierce western blotting substrate with the Pierce Western Blot Signal Enhancer?

The Enhancer will work for any chemiluminescent or colorimetric substrate.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does the Pierce Western Blot Signal Enhancer make the signal stronger?

The Enhancer relaxes the proteins, allowing the antibody to bind to the protein more efficiently.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use the Pierce Western Blot Signal Enhancer?

The Pierce Western Blot Signal Enhancer should be used after transferring proteins to the membrane and before blocking or staining the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.