Pierce™ Western Blot Signal Enhancer - FAQs

Western Blot信号增强剂不会和膜或蛋白质结合，因此不会干扰抗体和目的蛋白的结合。

剥离后的膜上仍有封闭试剂存留，所以你会得到不一致的结果。因此，我们建议仅在封闭和第一次膜杂交前使用Western Blot信号增强剂。

我们推荐在染膜和封闭前使用Western Blot信号增强剂。

Western Blot信号增强剂可以对任何发光或显色底物起作用。

Western Blot信号增强剂可以松弛蛋白，以便抗体和蛋白能更有效的结合。

Western Blot信号增强剂应在将蛋白质转膜后，封闭或染膜前使用。

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Enhancer does not bind to the membrane or proteins so it should not interfere with an antibody in binding the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After stripping the blot, blocking reagent remains on the membrane so you will get inconsistent results. Therefore, we recommend using the Enhancer only before blocking and the first probing of the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

We recommend using the Enhancer prior to staining and blocking.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Enhancer will work for any chemiluminescent or colorimetric substrate.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Enhancer relaxes the proteins, allowing the antibody to bind to the protein more efficiently.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Pierce Western Blot Signal Enhancer should be used after transferring proteins to the membrane and before blocking or staining the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.