Clean-Blot™ IP Detection Kit (HRP) - FAQs

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10 product FAQs found

使用Clean-Blot IP检测试剂后，能否对印迹膜进行剥离和再次检测？

可以。当使用化学发光底物时，可对膜进行剥离和再次检测，与使用二抗进行检测相似。

哪些底物可兼容Clean-Blot IP检测试剂？

为得到最佳结果，Clean-Blot IP检测可与SuperSignal底物或Thermo Scientific ECL蛋白质免疫印迹底物一起使用。当使用其他HRP或AP底物时，可根据经验确定Clean-Blot IP检测试剂的最佳浓度。

哪些封闭缓冲液可兼容Clean-Blot IP检测试剂？

Clean-Blot IP检测可兼容牛血清白蛋白、SuperBlock和StartingBlock封闭缓冲液和5%脱脂牛奶。可通过点印迹分析对其他封闭缓冲液的兼容性进行验证。

Clean-Blot IP检测试剂是否能够与所有抗体种类和亚类结合？

该试剂可与来自许多物种的IgG结合：牛IgG2，山羊IgG2，人IgG1、IgG2、IgG4，小鼠IgG2a、IgG2b、IgG3，大鼠IgG2c，绵羊IgG2，猪，狗和猫。该试剂不会与大鼠IgG2a和IgG2b或小鼠IgG1结合。如果怀疑该检测试剂会与某一抗体结合，可在正式实验前进行点印迹分析。

Clean-Blot IP检测试剂盒的原理是什么？

该检测利用HRP或AP偶联物与来自不同物种宿主的天然IgG结合，在免疫沉淀实验中获得清晰的特异性蛋白质免疫印迹检测结果，不受变性IgG的干扰。该试剂盒可作为二抗的替代品。

Can I strip and reprobe my blot after using the Clean-Blot IP Detection reagent?

Yes. When using a chemiluminescent substrate, membranes can be stripped and reprobed similar to probing with secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What substrates are compatible with the Clean-Blot IP Detection reagent?

For best results, use the the Clean-Blot IP Detection with SuperSignal Substrates or Thermo Scientific ECL Western Blotting Substrate. When using other HRP or AP substrates, empirically determine the optimal concentration of the Clean-Blot IP Detection Reagent.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What blocking buffers are compatible with the Clean-Blot IP Detection reagent?

The Clean-Blot IP Detection is compatible with bovine serum albumin, SuperBlock and StartingBlock Blocking Buffers and 5% nonfat milk. Verify compatibility with other blocking buffers by dot-blot analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does the Clean-Blot IP Detection Reagent bind to all antibody classes and subclasses?

The reagent binds to IgG from a wide range of species: bovine IgG2, goat IgG2, human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, IgG3, rat IgG2c, sheep IgG2, pig, dog and cat. It will not bind to rat IgG2a and IgG2b or mouse IgG1. If in doubt whether this detection reagent will bind to a specific antibody, perform a dot-blot analysis before the experiment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does the Clean-Blot IP Detection Kit work?

This assay uses a HRP conjugate or AP conjugate that binds to native IgG from various host species, allowing clear, specific Western blot detection from IP experiments without interference from denatured IgG. It is used simply as a substitute for secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.