EZ-Link TFPA-PEG3-Biotin (Cat. No. 21303) can be used in a wide variety of buffers. However, acidic pH and reducing conditions inactivate the aryl azide. We are unaware of any other photoreactive biotinylation reagents that could be used at low pH.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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Accepted Answer

EZ-Link TFPA-PEG3-Biotin (Cat. No. 21303) can be used in a wide variety of buffers. However, acidic pH and reducing conditions inactivate the aryl azide. We are unaware of any other photoreactive biotinylation reagents that could be used at low pH.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

EZ-Link™ TFPA-PEG3-Biotin - FAQs

EZ-Link TFPA-PEG3-Biotin (Cat. No. 21303) specifies to use within a pH range of 7-8. Unfortunately, the protein we are working with requires an acidic pH for stability and rapidly aggregates in neutral or basic pH. Can the photoactivation reaction be run at lower pH?