EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format - FAQs

查看更多产品信息 EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format - FAQs (21328)

3 个常见问题解答

Is there a cleavable analog of Sulfo-NHS-LC-Biotin?

Yes, we offer Sulfo-NHS-SS-Biotin (Cat. Nos. 21331, 21328). It has a disulfide bond in the spacer arm that can be cleaved with DTT or other reducing agents, thereby releasing the biotin tag from the previously labeled protein. This product is also available in kit format (Cat. Nos. 21945, 21445).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What product should I choose if I want to reversibly biotinylate a protein with a water soluble, amine-reactive biotin?

We recommend using EZ-Link Sulfo-NHS-SS Biotin (Cat. No. 21331 or 21338).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.