EZ-Link™ HPDP-Biotin, 50 mg - FAQs

查看更多产品信息 EZ-Link™ HPDP-Biotin, No-Weigh™ Format - FAQs (A35390, 21341)

2 个常见问题解答

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is it possible that HPDP-biotin (Cat. No. 21341) can also react with non-reduced sulfur?

We have no evidence that HPDP-biotin (or any pyridyldithiol compound) reacts with anything other than sulfhydryl (-SH) groups. However, many sulfur-containing compounds interconvert among several different forms. For thiouracil, with the different tautomers that are possible, the =S is in equilibrium with –SH, which can then react with the HPDP.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.