Site-specific incorporation of quadricyclane into a protein and photocleavage of the quadricyclane ligation adduct.
AuthorsTomlin FM, Gordon CG, Han Y, Wu TS, Sletten EM, Bertozzi CR
JournalBioorg Med Chem
PubMed ID29754834
'The quadricyclane (QC) ligation is a bioorthogonal reaction between a quadricyclane moiety and a nickel bis(dithiolene) derivative. Here we show that a QC amino acid can be incorporated into a protein site-specifically using the pyrrolysine-based genetic code expansion platform, and subsequently used for ligation chemistry. Additionally, we exploited the photolability ... More
Application of the fluctuation theorem for noninvasive force measurement in living neuronal axons.
AuthorsHayashi K, Tsuchizawa Y, Iwaki M, Okada Y
JournalMol Biol Cell
PubMed ID30281391
'Although its importance is recently widely accepted, force measurement has been difficult in living biological systems, mainly due to the lack of the versatile noninvasive force measurement methods. The fluctuation theorem, which represents the thermodynamic properties of small fluctuating nonequilibrium systems, has been applied to the analysis of the thermodynamic ... More
Capsular Polysaccharide (CPS) Release by Serotype 3 Pneumococcal Strains Reduces the Protective Effect of Anti-Type 3 CPS Antibodies.
AuthorsChoi EH, Zhang F, Lu YJ, Malley R
JournalClin Vaccine Immunol
PubMed ID26677201
The efficacy of the serotype 3 (ST3) pneumococcal conjugate vaccine (PCV) remains unclear. While the synthesis of capsular polysaccharide (CPS) of most serotypes is wzy dependent, the strains of two serotypes, 3 and 37, synthesize CPS by the synthase-dependent pathway, resulting in a polysaccharide that is not covalently linked to ... More
RNA Aptamers with Specificity for Heparosan and Chondroitin Glycosaminoglycans.
AuthorsKizer M, Li P, Cress BF, Lin L, Jing TT, Zhang X, Xia K, Linhardt RJ, Wang X
JournalACS Omega
PubMed ID30411046
In this study, two respective groups of RNA aptamers have been selected against two main classes of glycosaminoglycans (GAGs), heparosan, and chondroitin, as they have proven difficult to specifically detect in biological samples. GAGs are linear, anionic, polydisperse polysaccharides found ubiquitously in nature, yet their detection remains problematic. GAGs comprised ... More
Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
AuthorsPeng Y, Yu Y, Lin L, Liu X, Zhang X, Wang P, Hoffman P, Kim SY, Zhang F, Linhardt RJ
JournalGlycoconj J
PubMed ID29305777
Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour. In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry. The interaction of GAGs with collagen type II was assessed using surface plasmon resonance ... More
Major Differences between the Self-Assembly and Seeding Behavior of Heparin-Induced and in Vitro Phosphorylated Tau and Their Modulation by Potential Inhibitors.
AuthorsDespres C, Di J, Cantrelle FX, Li Z, Huvent I, Chambraud B, Zhao J, Chen J, Chen S, Lippens G, Zhang F, Linhardt R, Wang C, Klärner FG, Schrader T, Landrieu I, Bitan G, Smet-Nocca C
JournalACS Chem Biol
PubMed ID31046227
Self-assembly of the microtubule-associated protein tau into neurotoxic oligomers, fibrils, and paired helical filaments, and cell-to-cell spreading of these pathological tau species are critical processes underlying the pathogenesis of Alzheimer's disease and other tauopathies. Modulating the self-assembly process and inhibiting formation and spreading of such toxic species are promising strategies ... More
TGF-ß2 uses the concave surface of its extended finger region to bind betaglycan's ZP domain via three residues specific to TGF-ß and inhibin-a.
AuthorsHenen MA, Mahlawat P, Zwieb C, Kodali RB, Hinck CS, Hanna RD, Krzysiak TC, Ilangovan U, Cano KE, Hinck G, Vonberg M, McCabe M, Hinck AP
JournalJ Biol Chem
PubMed ID30598510
Betaglycan (BG) is a membrane-bound co-receptor of the TGF-ß family that selectively binds transforming growth factor-ß (TGF-ß) isoforms and inhibin A (InhA) to enable temporal-spatial patterns of signaling essential for their functions ... More