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引用和文献 (8)
Thermo Scientific™
EZ-Link™ NHS-PEG4 生物素化试剂盒
The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 1–10 mg protein labeling了解更多信息
| 货号 | 数量 |
|---|---|
| 21455 | 8 次反应试剂盒 |
货号 21455
价格（CNY)
5,986.00
飞享价
Ends: 31-Dec-2026
8,088.00共减 2,102.00 (26%)
Each
数量:
8 次反应试剂盒
价格（CNY)
5,986.00
飞享价
Ends: 31-Dec-2026
8,088.00共减 2,102.00 (26%)
Each
The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 1–10 mg protein labeling reactions. EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Features of EZ-Link NHS-PEG4-Biotin:
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules
NHS-PEG4-Biotin is a long (29.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Related Products
EZ-Link™ NHS-PEG4-Biotin
EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
Features of EZ-Link NHS-PEG4-Biotin:
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules
NHS-PEG4-Biotin is a long (29.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Related Products
EZ-Link™ NHS-PEG4-Biotin
EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
仅供科研使用。不可用于诊断程序。
规格
描述EZ-Link NHS-PEG4 生物素化试剂盒
标记规模1 至 10 mg
产品线EZ-Link
产品类型NHS-PEG4 生物素化试剂盒
数量8 次反应试剂盒
标记目标抗体, 蛋白
标签或染料生物素
溶解度水
间隔子聚乙二醇化
Unit SizeEach
内容与储存
足够用于：8 次标记反应，每次 1 至 10 mg 抗体
•EZ-Link NHS-PEG4-生物素，8 x 2 mg
•PBS 包（制成 500 mL），1 包
•Zeba 脱盐离心柱 (7K MWCO)，5 mL，10 个柱
•HABA 溶液，1 mL
•亲和素，10 mg
在 -20°C 下储存亲和素和生物素试剂微管。在室温下储存 PBS。在 4°C 下储存剩余的试剂盒组分。
•EZ-Link NHS-PEG4-生物素，8 x 2 mg
•PBS 包（制成 500 mL），1 包
•Zeba 脱盐离心柱 (7K MWCO)，5 mL，10 个柱
•HABA 溶液，1 mL
•亲和素，10 mg
在 -20°C 下储存亲和素和生物素试剂微管。在室温下储存 PBS。在 4°C 下储存剩余的试剂盒组分。
常见问题解答 (FAQ)
How do the EZ-Link Micro Biotinylation Kits differ from the standard biotinylation kits?
Can you provide the shelf-life for EZ-Link NHS-PEG4 Biotinylation Kit?
引用和文献 (8)
引用和文献
Abstract
Single-Molecule Arrays for Ultrasensitive Detection of Blood-Based Biomarkers for Immunotherapy.
Journal:Methods Mol Biol
PubMed ID:31502162
'Single-molecule array (Simoa) technology enables ultrasensitive protein detection that is suited to the development of peripheral blood-based assays for assessing immuno-oncology responses. We adapted a panel of Simoa assays to measure systemic cytokine levels from plasma and characterized physiologic variation in healthy individuals and preanalytic variation arising from processing and
Designing antibodies against LRRK2-targeted tau epitopes.
Journal:PLoS One
PubMed ID:30261006
'Phosphorylation of the microtubule associated protein tau is an important modulator of its normal physiological functioning; however, it may also contribute to tau mis-folding and aggregation in neurodegenerative diseases, which are collectively termed tauopathies. As such, the investigations of tau phosphorylation and kinases that modify tau are important in trying
B Cells Improve Overall Survival in HPV-Associated Squamous Cell Carcinomas and Are Activated by Radiation and PD-1 Blockade.
Journal:Clin Cancer Res
PubMed ID:32193227
'To characterize the role of B cells on human papilloma virus (HPV)-associated cancer patient outcomes and determine the effects of radiation and PD-1 blockade on B-cell populations.'
A small molecule inhibitor of PCSK9 that antagonizes LDL receptor binding via interaction with a cryptic PCSK9 binding groove.
Journal:Bioorg Med Chem
PubMed ID:32051094
'Proprotein convertase (PC) subtilisin kexin type 9 (PCSK9) inhibits the clearance of low density lipoprotein (LDL) cholesterol from plasma by directly interacting with the LDL receptor (LDLR). As the interaction promotes elevated plasma LDL cholesterol levels and a predisposition to cardiovascular disease (CVD), it has attracted much interest as a
Gpr158 mediates osteocalcin's regulation of cognition.
Journal:J Exp Med
PubMed ID:28851741
That osteocalcin (OCN) is necessary for hippocampal-dependent memory and to prevent anxiety-like behaviors raises novel questions. One question is to determine whether OCN is also sufficient to improve these behaviors in wild-type mice, when circulating levels of OCN decline as they do with age. Here we show that the presence