Pierce™ GST 蛋白相互作用 Pull-Down 试剂盒
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Pierce™ GST 蛋白相互作用 Pull-Down 试剂盒
Thermo Scientific™

Pierce™ GST 蛋白相互作用 Pull-Down 试剂盒

Thermo Fisher Scientific Pierce GST 标签蛋白相互作用沉降试剂盒包含捕获并纯化与 GST 标签融合蛋白相互作用的蛋白所需的组分。GST 标签蛋白相互作用沉降试剂盒的特点:•GST了解更多信息
Have Questions?
货号 21516
价格(CNY)
5,109.00
飞享价
Ends: 31-Dec-2025
6,914.00
共减 1,805.00 (26%)
Each
添加至购物车
请求批量或定制报价
价格(CNY)
5,109.00
飞享价
Ends: 31-Dec-2025
6,914.00
共减 1,805.00 (26%)
Each
添加至购物车
Thermo Fisher Scientific Pierce GST 标签蛋白相互作用沉降试剂盒包含捕获并纯化与 GST 标签融合蛋白相互作用的蛋白所需的组分。

GST 标签蛋白相互作用沉降试剂盒的特点:

GST 沉降(产品编号 21516)—温和纯化任何 GST 标签融合蛋白(未变性)的蛋白相互作用体
全套试剂盒—可提供纯化蛋白:蛋白相互作用的所有组分和详细方案
无需特殊设备—使用常用实验室设备和试剂(例如,微量离心机)
便利—量离心柱有助于琼脂糖微珠的简单高效操作,包括简单的多样品处理
灵活—指示包括与许多不同来源的诱饵和猎物蛋白配合使用的方案
结合—每毫升树脂中结合大约 8 mg GST 标签融合蛋白

应用:
•发现细胞裂解物的一种新蛋白:蛋白相互作用的蛋白
•确认来自细胞裂解物或先前纯化的蛋白的假定相互作用
•从体外转录/翻译裂解物中提取蛋白:蛋白相互作用信息

您提供标签融合蛋白作为“诱饵”和表达假定蛋白相互作用靶标(“猎物”)的细胞,沉降试剂盒提供其他:细胞裂解缓冲液、微量离心柱、标签特异性亲和树脂(琼脂糖微珠)和优化缓冲液和方案。沉降试剂盒设计用于向初次用户教授该方法并提高经验丰富的研究人员的易用性、便利性和可重现性。

相关产品
Pierce™ His 蛋白相互作用沉降试剂盒
For Research Use Only. Not for use in diagnostic procedures.

Pull-down是一种小规模亲和纯化技术,与免疫沉淀反应类似,但其抗体功能被某些其他亲和系统代替。在这种情况下,亲和系统是一种可被谷胱甘肽琼脂糖微珠捕获的 GST 标签蛋白。融合标记蛋白质作为捕获推定连结配偶体(即猎物)的诱饵。在典型的pull-down 试验中,固定的诱饵蛋白质与细胞裂解液一起孵育。在规定的洗涤步骤后,相互作用体进行选择性洗脱,以便进行凝胶内分析或 Western 印迹分析。

规格
检测亲和纯化
柱床容量750 μL
适用于(应用)沉降(基于标签)
适用于(设备)小型离心机
产品规格试剂盒
配方Slurry: 50 %
数量1 个试剂盒
足够用于25 次反应
靶标无靶标特异性
容量(公制)1.5 mL
产品线Pierce™
类型沉降试剂盒
Unit SizeEach

常见问题解答 (FAQ)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.