Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.
AuthorsKeinan N, Tyomkin D, Shoshan-Barmatz V
JournalMol Cell Biol
PubMed ID20937774
'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More
Structure-based analysis of VDAC1 protein: defining oligomer contact sites.
AuthorsGeula S, Naveed H, Liang J, Shoshan-Barmatz V
JournalJ Biol Chem
PubMed ID22117062
'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More
Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex.
AuthorsSchulz C, Lytovchenko O, Melin J, Chacinska A, Guiard B, Neumann P, Ficner R, Jahn O, Schmidt B, Rehling P
JournalJ Cell Biol
PubMed ID22065641
N-terminal targeting signals (presequences) direct proteins across the TOM complex in the outer mitochondrial membrane and the TIM23 complex in the inner mitochondrial membrane. Presequences provide directionality to the transport process and regulate the transport machineries during translocation. However, surprisingly little is known about how presequence receptors interact with the ... More
Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46.
AuthorsLucker BF, Miller MS, Dziedzic SA, Blackmarr PT, Cole DG
JournalJ Biol Chem
PubMed ID20435895
Intraflagellar transport (IFT) particles of Chlamydomonas reinhardtii contain two distinct protein complexes, A and B, composed of at least 6 and 15 protein subunits, respectively. As isolated from C. reinhardtii flagella, IFT complex B can be further reduced to a approximately 500-kDa core that contains IFT88, 2x IFT81, 2x IFT74/72, ... More
Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A.
AuthorsSheridan JT, Worthington EN, Yu K, Gabriel SE, Hartzell HC, Tarran R
JournalJ Biol Chem
PubMed ID21056985
Members of the Anoctamin (Ano)/TMEM16A family have recently been identified as essential subunits of the Ca(2+)-activated chloride channel (CaCC). For example, Ano1 is highly expressed in multiple tissues including airway epithelia, where it acts as an apical conduit for transepithelial Cl(-) secretion and helps regulate lung liquid homeostasis and mucus ... More
Proteins at the polypeptide tunnel exit of the yeast mitochondrial ribosome.
AuthorsGruschke S, Gröne K, Heublein M, Hölz S, Israel L, Imhof A, Herrmann JM, Ott M
JournalJ Biol Chem
PubMed ID20404317
Oxidative phosphorylation in mitochondria requires the synthesis of proteins encoded in the mitochondrial DNA. The mitochondrial translation machinery differs significantly from that of the bacterial ancestor of the organelle. This is especially evident from many mitochondria-specific ribosomal proteins. An important site of the ribosome is the polypeptide tunnel exit. Here, ... More