Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.
AuthorsKeinan N, Tyomkin D, Shoshan-Barmatz V
JournalMol Cell Biol
PubMed ID20937774
'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More
Structure-based analysis of VDAC1 protein: defining oligomer contact sites.
AuthorsGeula S, Naveed H, Liang J, Shoshan-Barmatz V
JournalJ Biol Chem
PubMed ID22117062
'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More
Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes.
AuthorsMokry M, Hatzis P, Schuijers J, Lansu N, Ruzius FP, Clevers H, Cuppen E
JournalNucleic Acids Res
PubMed ID21914722
'Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RNA polymerase II (Pol II), ... More
LXRa regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8.
AuthorsPourcet B, Feig JE, Vengrenyuk Y, Hobbs AJ, Kepka-Lenhart D, Garabedian MJ, Morris SM, Fisher EA, Pineda-Torra I
JournalCirc Res
PubMed ID21757649
Activation of liver X receptors (LXRs) inhibits the progression of atherosclerosis and promotes regression of existing lesions. In addition, LXRa levels are high in regressive plaques. Macrophage arginase 1 (Arg1) expression is inversely correlated with atherosclerosis progression and is markedly decreased in foam cells within the lesion. ... More
Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.
AuthorsGogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D
JournalJ Biol Chem
PubMed ID21712378
Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More