BS(PEG)5（聚乙二醇化二(磺基琥珀酰亚胺)辛二酸）
BS(PEG)5（聚乙二醇化二(磺基琥珀酰亚胺)辛二酸）
引用和文献 (3)
Thermo Scientific™

BS(PEG)5（聚乙二醇化二(磺基琥珀酰亚胺)辛二酸）

BS(PEG)5 是一种双琥珀酰亚胺酯活化的 PEG 化合物，用于蛋白和其他分子中的伯胺 (—NH2) 之间的交联。PEG5 间隔两端的 N-羟基琥珀酰亚胺酯 (NHS)了解更多信息
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货号数量
21581100 mg
A3539610 x 1 mg
货号 21581
价格（CNY)
6,973.00
Each
添加至购物车
数量:
100 mg
请求批量或定制报价
价格（CNY)
6,973.00
Each
添加至购物车
BS(PEG)5 是一种双琥珀酰亚胺酯活化的 PEG 化合物，用于蛋白和其他分子中的伯胺 (—NH2) 之间的交联。PEG5 间隔两端的 N-羟基琥珀酰亚胺酯 (NHS) 基团在 pH 7-9 条件下与赖氨酸和 N 端氨基基团发生特异性高效反应，形成稳定的酰胺键。

Thermo Scientific No-Weigh 产品是以预分装形式提供的专业试剂。预称重的包装可消除重复开瓶和关盖，从而防止随时间推移试剂反应性的降低和污染。对于这种规格，可每次使用一小瓶新鲜试剂，消除了对少量试剂进行称重的麻烦，并减少了对试剂稳定性的担忧。

BS(PEG)5 的特点：

•反应性基团：磺基-NHS 酯（两端）
•可与以下基团发生反应：氨基基团（伯胺）
• NHS 酯在 pH 7-9 条件下与 —NH2 基团反应形成稳定的酰胺键
• 水溶性；与非聚乙二醇化 BS3（也具有可溶性）和 DSS（水不溶性）具有可比性
• 具有膜不通透性，可进行细胞表面标记
• 用灵活的 PEG 间隔臂可不可逆地交联蛋白或多肽
• 聚乙二醇间隔臂有助于保持偶联物的溶解度
• 具有明确结构和分子量的纯化合物，确保可重现的蛋白修饰效应
• PEG 间隔具有独特的优势，包括更高的稳定性、更低的积聚倾向和更低的免疫原性
• 适用于小分子或肽偶联

BS(PEG)n 的其他名称：

•二-NHS-dPEG
•NHS-PEG-NHS
•BS(PEO)5
• 二(NHS)PEO5
• BS-PEO9
• dPEG NHS 酯
• 二-琥珀酰亚胺酯 PEG 400

产品参考
交联剂应用指南 -- 检索本产品的最新参考文献
Bioconjugate Techniques, Academic Press（产品编号 20036）

相关产品
BS(PEG)9（聚乙二醇化二(磺基琥珀酰亚胺)辛二酸）
BS3（二(磺基琥珀酰亚胺)辛二酸）
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性否
描述BS(PEG)5
形式液体
标记方法化学标记
分子量532.5
聚乙二醇化是
产品线Pierce
数量100 mg
反应一部分NHS 酯
运输条件环境
溶解度水
间隔臂长21.7 Å
水溶性是
化学反应性胺-胺
可裂解否
交联剂类型同型双功能团试剂
产品规格标准、一次性使用
产品类型交联剂
间隔子中等（10 至 30 Å）
Unit SizeEach
内容与储存
接收后在 -20°C 下干燥储存。

常见问题解答 (FAQ)

Can you provide the shelf-life for BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)?

BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you recommend crosslinking reagents for viral capsids (diameter 30 nm)?

Amine-to-amine crosslinkers are a good choice for your application since amino groups are usually abundant on the surface of proteins. Our most popular amine-reactive homobifunctional crosslinker that is water-soluble is BS3 (Cat. No. A39266). This has a moderate-length (11.4 A) linker that is good for linking adjacent proteins. If you need the ability to cleave the linker with sulfhydryl reducing agents I would suggest DTSSP (Cat. No. 21578). If you think you might need an extra-long crosslinker, then BS(PEG)5 (Cat. No. 21581) or BS(PEG)9 (Cat. No. 21582) are alternatives.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (3)

引用和文献
Abstract
Nonpolarized signaling reveals two distinct modes of 3D cell migration.
Authors:Petrie RJ, Gavara N, Chadwick RS, Yamada KM
Journal:J Cell Biol
PubMed ID:22547408
'We search in this paper for context-specific modes of three-dimensional (3D) cell migration using imaging for phosphatidylinositol (3,4,5)-trisphosphate (PIP3) and active Rac1 and Cdc42 in primary fibroblasts migrating within different 3D environments. In 3D collagen, PIP3 and active Rac1 and Cdc42 were targeted to the leading edge, consistent with lamellipodia-based ... More
A force-based protein biochip.
Authors:Blank K, Mai T, Gilbert I, Schiffmann S, Rankl J, Zivin R, Tackney C, Nicolaus T, Spinnler K, Oesterhelt F, Benoit M, Clausen-Schaumann H, Gaub HE
Journal:Proc Natl Acad Sci U S A
PubMed ID:12975526
A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand-receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small ... More
Dimerization of plant defensin NaD1 enhances its antifungal activity.
Authors:Lay FT, Mills GD, Poon IK, Cowieson NP, Kirby N, Baxter AA, van der Weerden NL, Dogovski C, Perugini MA, Anderson MA, Kvansakul M, Hulett MD
Journal:J Biol Chem
PubMed ID:22511788
The plant defensin, NaD1, from the flowers of Nicotiana alata, is a member of a family of cationic peptides that displays growth inhibitory activity against several filamentous fungi, including Fusarium oxysporum. The antifungal activity of NaD1 has been attributed to its ability to permeabilize membranes; however, the molecular basis of ... More