Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
Authorsvan Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
JournalJ Biol Chem
PubMed ID22493445
'Genetic and molecular studies suggest that activin receptor-like kinase 1 (ALK1), a transforming growth factor ß (TGF-ß) type I receptor, and endoglin, a TGF-ß co-receptor, play an essential role in vascular development and pathological angiogenesis. Several agents that interfere with ALK1 and endoglin function are currently in clinical trials for ... More
The MUC1-C oncoprotein binds to the BH3 domain of the pro-apoptotic BAX protein and blocks BAX function.
AuthorsAhmad R, Alam M, Rajabi H, Kufe D
JournalJ Biol Chem
PubMed ID22544745
'The pro-apoptotic BAX protein contains a BH3 domain that is necessary for its dimerization and for activation of the intrinsic apoptotic pathway. The MUC1 (mucin 1) heterodimeric protein is overexpressed in diverse human carcinomas and blocks apoptosis in the response to stress. In this study, we demonstrate that the oncogenic ... More
Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
AuthorsYao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Boström KI,
JournalBlood
PubMed ID22474252
The importance of morphogenetic proteins (BMPs) and their antagonists in vascular development is increasingly being recognized. BMP-4 is essential for angiogenesis and is antagonized by matrix Gla protein (MGP) and crossveinless 2 (CV2), both induced by the activin receptor like-kinase 1 (ALK1) when stimulated by BMP-9. In this study, however, ... More
Crosslinking-MS analysis reveals RNA polymerase I domain architecture and basis of rRNA cleavage.
AuthorsJennebach S, Herzog F, Aebersold R, Cramer P
JournalNucleic Acids Res
PubMed ID22396529
RNA polymerase (Pol) I contains a 10-subunit catalytic core that is related to the core of Pol II and includes subunit A12.2. In addition, Pol I contains the heterodimeric subcomplexes A14/43 and A49/34.5, which are related to the Pol II subcomplex Rpb4/7 and the Pol II initiation factor TFIIF, respectively. ... More
Binding of calmodulin to the D2-dopamine receptor reduces receptor signaling by arresting the G protein activation switch.
AuthorsBofill-Cardona E, Kudlacek O, Yang Q, Ahorn H, Freissmuth M, Nanoff C
JournalJ Biol Chem
PubMed ID10926927
Signaling by D(2)-dopamine receptors in neurons likely proceeds in the presence of Ca(2+) oscillations. We describe here the biochemical basis for a cross-talk between intracellular Ca(2+) and the D(2) receptor. By activation of calmodulin (CaM), Ca(2+) directly inhibits the D(2) receptor; this conclusion is based on the following observations: (i) ... More
Opiate antagonist prevents µ- and d-opiate receptor dimerization to facilitate ability of agonist to control ethanol-altered natural killer cell functions and mammary tumor growth.
AuthorsSarkar DK, Sengupta A, Zhang C, Boyadjieva N, Murugan S
JournalJ Biol Chem
PubMed ID22451667
In the natural killer (NK) cells, d-opiate receptor (DOR) and µ-opioid receptor (MOR) interact in a feedback manner to regulate cytolytic function with an unknown mechanism. Using RNK16 cells, a rat NK cell line, we show that MOR and DOR monomer and dimer proteins existed in these cells and that ... More
Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane.
AuthorsFaye A, Esnous C, Price NT, Onfray MA, Girard J, Prip-Buus C
JournalJ Biol Chem
PubMed ID17650509
Carnitine palmitoyltransferase (CPT) 1A catalyzes the rate-limiting step in the transport of long chain acyl-CoAs from cytoplasm to the mitochondrial matrix by converting them to acylcarnitines. Located within the outer mitochondrial membrane, CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor. In this study, we investigate for the first time ... More