'TNF-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor (FGF)-inducible 14 (Fn14) are a TNF superfamily ligand-receptor pair involved in many cellular processes including proliferation, migration, differentiation, inflammation, and angiogenesis. The Fn14 receptor is expressed at relatively low levels in normal tissues, but it is known to be dramatically ... More
Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3.
'The multiple transferable resistance (mTR) pump from Neisseria gonorrhoeae MtrCDE multidrug pump is assembled from the inner and outer membrane proteins MtrD and MtrE and the periplasmic membrane fusion protein MtrC. Previously we established that while there is a weak interaction of MtrD and MtrE, MtrC binds with relatively high ... More
Winding single-molecule double-stranded DNA on a nanometer-sized reel.
AuthorsYou H, Iino R, Watanabe R, Noji H
JournalNucleic Acids Res
PubMed ID22772992
A molecular system of a nanometer-sized reel was developed from F(1)-ATPase, a rotary motor protein. By combination with magnetic tweezers and optical tweezers, single-molecule double-stranded DNA (dsDNA) was wound around the molecular reel. The bending stiffness of dsDNA was determined from the winding tension (0.9-6.0 pN) and the diameter of ... More
Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression.
AuthorsPallaoro A, Braun GB, Moskovits M
JournalProc Natl Acad Sci U S A
PubMed ID21930955
A multiplexed, ratiometric method is described that can confidently distinguish between cancerous and noncancerous epithelial prostate cells in vitro. The technique is based on bright surface-enhanced resonance Raman scattering (SERRS) biotags (SBTs) infused with unique Raman reporter molecules, and carrying cell-specific peptides. Two sets of SBTs were used. One targets ... More
A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps.
AuthorsLobedanz S, Bokma E, Symmons MF, Koronakis E, Hughes C, Koronakis V
JournalProc Natl Acad Sci U S A
PubMed ID17360572
Bacteria such as Escherichia coli and Pseudomonas aeruginosa expel antibiotics and other inhibitors via tripartite multidrug efflux pumps spanning the inner and outer membranes and the intervening periplasmic space. A key event in pump assembly is the recruitment of an outer membrane-anchored TolC exit duct by the adaptor protein of ... More
Dendritic cell-directed CTLA-4 engagement during pancreatic beta cell antigen presentation delays type 1 diabetes.
AuthorsKarumuthil-Melethil S, Perez N, Li R, Prabhakar BS, Holterman MJ, Vasu C
JournalJ Immunol
PubMed ID20483724
The levels of expression of alternatively spliced variants of CTLA-4 and insufficient CTLA-4 signaling have been implicated in type 1 diabetes. Hence, we hypothesized that increasing CTLA-4-specific ligand strength on autoantigen-presenting dendritic cells (DCs) can enhance ligation of CTLA-4 on T cells and lead to modulation of autoreactive T cell ... More