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引用和文献 (4)
Thermo Scientific™
AMAS（N-α-马来酰亚胺基乙酰-氧基琥珀酰亚胺酯）
Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a了解更多信息
| 货号 | 数量 |
|---|---|
| 22295 | 50 mg |
货号 22295
价格（CNY)
3,163.00
Each
数量:
50 mg
价格（CNY)
3,163.00
Each
Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).
Features of AMAS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Shortest of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Very short aliphatic spacer has low potential for eliciting an immune response
Features of AMAS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Shortest of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Very short aliphatic spacer has low potential for eliciting an immune response
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性是
描述AMAS
形式粉末
标记方法化学标记
分子量252.18
聚乙二醇化否
产品线Pierce
数量50 mg
反应一部分马来酰亚胺、NHS 酯
运输条件环境
溶解度DMF、DMSO
间隔臂长4.4 Å
水溶性否
化学反应性胺-巯基
可裂解否
交联剂类型异型双功能团试剂
产品规格标准
产品类型交联剂
间隔子短 (<10 Å)
Unit SizeEach
内容与储存
接收后，请在 4°C 下干燥储存。
常见问题解答 (FAQ)
Can you provide the shelf-life for AMAS (N-α-maleimidoacet-oxysuccinimide ester)?
引用和文献 (4)
引用和文献
Abstract
Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome.
Journal:J Biol Chem
PubMed ID:20410297
'Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is present as a homodimer in archaea and as a highly conserved heterodimer in eukaryotes. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila
Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation.
Journal:J Biol Chem
PubMed ID:16257976
'The transcription factor RovA of Yersinia pseudotuberculosis and analogous proteins in other Enterobacteriaceae activate the expression of virulence genes that play a crucial role in stress adaptation and pathogenesis. In this study, we demonstrate that the RovA protein forms dimers independent of DNA binding, stimulates RNA polymerase, most likely via
A central role for the T1 domain in voltage-gated potassium channel formation and function.
Journal:J Biol Chem
PubMed ID:11312262
To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in
Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents.
Journal:J Biol Chem
PubMed ID:12972413
Heterobifunctional thiol to amine cross-linking agents were used to gain new insights on the dynamics and conformational factors governing the interaction between the cardiac Ca2+ pump (SERCA2a) and phospholamban (PLB). PLB is a small protein inhibitor of SERCA2a that reduces enzyme affinity for Ca2+ and thereby regulates cardiac contractility. We