Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Covalent attachment of synthetic DNA to self-assembled monolayer films.
AuthorsChrisey LA, Lee GU, O'Ferrall CE
JournalNucleic Acids Res
PubMed ID8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Recognition of galactan components of pectin by galectin-3.
AuthorsGunning AP, Bongaerts RJ, Morris VJ
JournalFASEB J
PubMed ID18832596
It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this ... More
Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver.
AuthorsLee SJ, Park SY, Jung MY, Bae SM, Kim IS
JournalBlood
PubMed ID21427291
Aged or damaged RBCs are effectively removed from the blood circulation by Kupffer cells in the liver, but little is known regarding the mechanism of the clearance process. Here we show that stabilin-1 and stabilin-2 in hepatic sinusoidal endothelial cells (HSECs) are critical in effectively clearing damaged RBCs in mouse ... More
Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy.
AuthorsGunning AP, Chambers S, Pin C, Man AL, Morris VJ, Nicoletti C
JournalFASEB J
PubMed ID18263697
Specific molecular-receptor interactions with gut epithelium cells are important in understanding bioactivity of food components and drugs, binding of commensal microflora, attachment and initiation of defense mechanisms against pathogenic bacteria and for development of targeted delivery systems to the gut. However, methods for probing such interactions are lacking. Methodology has ... More
Obox4 critically regulates cAMP-dependent meiotic arrest and MI-MII transition in oocytes.
AuthorsLee HS, Kim EY, Kim KH, Moon J, Park KS, Kim KS, Lee KA
JournalFASEB J
PubMed ID20154267
Extra follicular oocytes spontaneously resume meiosis in vitro, but the intact germinal vesicle (GV) is retained if the oocytes are cultured in medium containing phosphodiesterase (PDE) inhibitors or cAMP analogues. On the basis of our finding that Obox4 is prominently expressed in oocytes, the present study was conducted to determine ... More
Preparation of monoclonal antibodies against N-(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated acetylspermine, and development of an enzyme-linked immunosorbent assay (ELISA) for N1,N12-diacetylspermine.
AuthorsFujiwara K, Kaminishi Y, Kitagawa T, Tsuru D, Yabuuchi M, Kanetake H, Nomata K
JournalJ Biochem
PubMed ID9644270
We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2b, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(gamma-maleimidobutyryloxy)succinimide (GMBS). Among these mAbs, ACSPM-2 was the most useful for the development ... More