Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Substrate-induced conformational change in bacterial complex I.
AuthorsMamedova AA, Holt PJ, Carroll J, Sazanov LA
JournalJ Biol Chem
PubMed ID15037611
'The mechanism coupling electron transfer and proton pumping in respiratory complex I (NADH-ubiquinone oxidoreductase) has not been established, but it has been suggested that it involves conformational changes. Here, the influence of substrates on the conformation of purified complex I from Escherichia coli was studied by cross-linking and electron microscopy. ... More
Chondrex: new marker of joint disease.
AuthorsHarvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C
JournalClin Chem
PubMed ID9510855
Chondrex, a major secretory protein of human chondrocytes and synovial fibroblasts, is increased in serum of patients with joint and cartilage disease. We have developed a sandwich-type ELISA for quantifying chondrex in serum. The interassay CVs were 2.8-3.7% and the average within-run and total CVs were 3.6% and 5.4%, respectively. ... More
Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma.
AuthorsPercy AJ, Trainor DA, Rittenhouse J, Phelps J, Koda JE
JournalClin Chem
PubMed ID8605675
Amylin is a 37-amino-acid polypeptide synthesized in and secreted from pancreatic beta cells along with insulin. Its biological actions include the slowing and reduction of postmeal increases in plasma glucose concentrations. Studies of the basic amylin biology in humans have been hampered by the lack of a rapid, sensitive assay ... More
Sulfhydryl-based tumor antigen-carrier protein conjugates stimulate superior antitumor immunity against B cell lymphomas.
AuthorsBetting DJ, Kafi K, Abdollahi-Fard A, Hurvitz SA, Timmerman JM
JournalJ Immunol
PubMed ID18768870
Therapeutic vaccination of B cell lymphoma patients with tumor-specific Ig (idiotype, or Id) chemically coupled to the immunogenic foreign carrier protein keyhole limpet hemocyanin (KLH) using glutaraldehyde has shown promising results in early clinical trials, and phase III trials are underway. However, glutaraldehyde Id-KLH vaccines fail to elicit anti-Id immune ... More