Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Ligand-receptor and receptor-receptor interactions act in concert to activate signaling in the Drosophila toll pathway.
AuthorsWeber AN, Moncrieffe MC, Gangloff M, Imler JL, Gay NJ
JournalJ Biol Chem
PubMed ID15795223
In Drosophila, the signaling pathway mediated by the Toll receptor is critical for the establishment of embryonic dorso-ventral pattern and for innate immune responses to bacterial and fungal pathogens. Toll is activated by high affinity binding of the cytokine Spätzle, a dimeric ligand of the cystine knot family. In vertebrates, ... More
Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A.
AuthorsSheridan JT, Worthington EN, Yu K, Gabriel SE, Hartzell HC, Tarran R
JournalJ Biol Chem
PubMed ID21056985
Members of the Anoctamin (Ano)/TMEM16A family have recently been identified as essential subunits of the Ca(2+)-activated chloride channel (CaCC). For example, Ano1 is highly expressed in multiple tissues including airway epithelia, where it acts as an apical conduit for transepithelial Cl(-) secretion and helps regulate lung liquid homeostasis and mucus ... More
Studying the protein organization of the postsynaptic density by a novel solid phase- and chemical cross-linking-based technology.
Agarose beads carrying a cleavable, fluorescent, and photoreactive cross-linking reagent on the surface were synthesized and used to selectively pull out the proteins lining the surface of supramolecules. A quantitative comparison of the abundances of various proteins in the sample pulled out by the beads from supramolecules with their original ... More
Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane.
AuthorsFaye A, Esnous C, Price NT, Onfray MA, Girard J, Prip-Buus C
JournalJ Biol Chem
PubMed ID17650509
Carnitine palmitoyltransferase (CPT) 1A catalyzes the rate-limiting step in the transport of long chain acyl-CoAs from cytoplasm to the mitochondrial matrix by converting them to acylcarnitines. Located within the outer mitochondrial membrane, CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor. In this study, we investigate for the first time ... More