Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function.
AuthorsSimonin A, Fuster D
JournalJ Biol Chem
PubMed ID20855896
'The ubiquitously expressed mammalian Na(+)/H(+) exchanger 1 (NHE1) controls cell volume and pH but is also critically involved in complex biological processes like cell adhesion, cell migration, cell proliferation, and mechanosensation. Pathways controlling NHE1 turnover at the plasma membrane, however, are currently unclear. Here, we demonstrate that NHE1 undergoes ubiquitylation ... More
The extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex.
AuthorsZhang H, Pradhan P, Kaur P
JournalJ Biol Chem
PubMed ID20876527
'Two novel regulatory motifs, LDEVFL and C-terminal regulatory Glu (E)-rich motif (CREEM), are identified in the extreme C terminus of the ABC protein DrrA, which is involved in direct interaction with the N-terminal cytoplasmic tail of the membrane protein DrrB and in homodimerization of DrrA. Disulfide cross-linking analysis showed that ... More
Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin.
AuthorsSmith AL, Dohn MR, Brown MV, Reynolds AB
JournalMol Biol Cell
PubMed ID22031287
The dynamic functional linkage of cadherins with the underlying actin cytoskeleton is tightly regulated to achieve proper cell-cell adhesion. p120-catenin (p120) regulates both cadherin stability and actin dynamics, but the relationship between these two functions remains unclear. Using a novel proteomic approach called reversible cross-link immunoprecipitation, or ReCLIP, we previously ... More
Claudin-2 forms homodimers and is a component of a high molecular weight protein complex.
AuthorsVan Itallie CM, Mitic LL, Anderson JM
JournalJ Biol Chem
PubMed ID21098027
Tight junctions are multiprotein complexes that form the fundamental physiologic and anatomic barrier between epithelial and endothelial cells, yet little information is available about their molecular organization. To begin to understand how the transmembrane proteins of the tight junction are organized into multiprotein complexes, we used blue native-PAGE (BN-PAGE) and ... More
Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.
AuthorsAuclair JR, Boggio KJ, Petsko GA, Ringe D, Agar JN
JournalProc Natl Acad Sci U S A
PubMed ID21098299
Amyotrophic lateral sclerosis (ALS) is a disorder characterized by the death of both upper and lower motor neurons and by 3- to 5-yr median survival postdiagnosis. The only US Food and Drug Administration-approved drug for the treatment of ALS, Riluzole, has at best, moderate effect on patient survival and quality ... More