BCL-2 selectively interacts with the BID-induced open conformer of BAK, inhibiting BAK auto-oligomerization.
AuthorsRuffolo SC, Shore GC
JournalJ Biol Chem
PubMed ID12721291
'Caspase-8 cleaves BID to tBID, which targets mitochondria and induces oligomerization of BAX and BAK within the outer membrane, resulting in release of cytochrome c from the organelle. Here, we have initiated these steps in isolated mitochondria derived from control and BCL-2-overexpressing cells using synthetic BH3 peptides and subsequently analyzed ... More
Small molecule obatoclax (GX15-070) antagonizes MCL-1 and overcomes MCL-1-mediated resistance to apoptosis.
AuthorsNguyen M, Marcellus RC, Roulston A, Watson M, Serfass L, Murthy Madiraju SR, Goulet D, Viallet J, Bélec L, Billot X, Acoca S, Purisima E, Wiegmans A, Cluse L, Johnstone RW, Beauparlant P, Shore GC
JournalProc Natl Acad Sci U S A
PubMed ID18040043
'Elevated expression of members of the BCL-2 pro-survival family of proteins can confer resistance to apoptosis in cancer cells. Small molecule obatoclax (GX15-070), which is predicted to occupy a hydrophobic pocket within the BH3 binding groove of BCL-2, antagonizes these members and induces apoptosis, dependent on BAX and BAK. Reconstitution ... More
Hybridization of an immobilized PNA probe with its complementary oligodeoxyribonucleotide on the surface of silica glass.
AuthorsMasuko M
JournalNucleic Acids Res Suppl
PubMed ID14510422
A 16mer sequence of peptide nucleic acid (PNA) was immobilized onto the surface of silica glass to supply solid supports for DNA chip and biosensor constructions. To the surface, 3-aminopropyltriethoxysilane (APTES), succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate) (LC-SMCC), and cysteinyl-PNA were introduced in order. Hybridization of the immobilized PNA with its complementary oligodeoxyribonucleotide (ODN) should ... More