Folding of the striated muscle myosin motor domain.
Authors Chow Diana; Srikakulam Rajani; Chen Ying; Winkelmann Donald A;
JournalJ Biol Chem
PubMed ID12110670
'We have investigated the folding of the myosin motor domain using a chimera of an embryonic striated muscle myosin II motor domain fused on its COOH terminus to a thermal stable, fast folding variant of green fluorescent protein (GFP). In in vitro expression assays, the GFP domain of the chimeric ... More
Role of the E1A Rb-binding domain in repression of the NF-kappa B-dependent defense against tumor necrosis factor-alpha.
Authors Cook James L; Walker Thomas A; Worthen G Scott; Radke Jay R;
JournalProc Natl Acad Sci U S A
PubMed ID12119420
'The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblastoma (Rb)-family proteins, but the roles of E1A interactions with ... More
Induction of bacterial lipoprotein tolerance is associated with suppression of toll-like receptor 2 expression.
AuthorsWang JH, Doyle M, Manning BJ, Di Wu Q, Blankson S, Redmond HP.
JournalJ Biol Chem
PubMed ID12133836
'Tolerance to bacterial cell wall components including lipopolysaccharide (LPS) may represent an essential regulatory mechanism during bacterial infection. Two members of the Toll-like receptor (TLR) family, TLR2 and TLR4, recognize the specific pattern of bacterial cell wall components. TLR4 has been found to be responsible for LPS tolerance. However, the ... More
Hepatitis C virus subgenomic replicons in the human embryonic kidney 293 cell line.
AuthorsAli S, Pellerin C, Lamarre D, Kukolj G,
JournalJ Virol
PubMed ID14671129
'Hepatitis C virus (HCV) infects liver cells and its replication in other cells is incompletely defined. Human hepatoma Huh-7 cells harboring subgenomic HCV replicons were used in somatic cell fusion experiments with human embryonic kidney 293 cells as a means of examining the permissiveness of 293 cells for HCV subgenomic ... More
c-Myc sensitizes cells to tumor necrosis factor-mediated apoptosis by inhibiting nuclear factor kappa B transactivation.
Authors You Zongbing; Madrid Lee V; Saims Daniel; Sedivy John; Wang Cun-Yu;
JournalJ Biol Chem
PubMed ID12149248
Nuclear factor kappaB (NF-kappaB) plays a key role in suppression of tumor necrosis factor (TNF)-mediated apoptosis by inducing a variety of anti-apoptotic genes. Expression of c-Myc has been shown to sensitize cells to TNF-mediated apoptosis by inhibiting NF-kappaB activation. However, the precise step in the NF-kappaB signaling pathway and apoptosis ... More
Three adjacent serines in the extracellular domains of the CaR are required for L-amino acid-mediated potentiation of receptor function.
Authors Zhang Zaixiang; Qiu Weiying; Quinn Stephen J; Conigrave Arthur D; Brown Edward M; Bai Mei;
JournalJ Biol Chem
PubMed ID12095982
The extracellular calcium (Ca(2+)(o))-sensing receptor (CaR) is a key player in Ca(2+)(o) homeostasis. The activity of CaR can be potentiated by various l-amino acids. In this study, we examined whether conserved amino acid residues involved in the binding of glutamate to metabotropic glutamate receptors (mGluRs) also participate in the potentiation ... More