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查看更多产品信息 Pierce™ Power Stain Cassette - FAQs (22836)
12 个常见问题解答
染色步骤中使用超过50%的酒精，会使凝胶脱水。只需将凝胶放在水中，使其再水化。
不能。每种盒子只能用于其预期使用目的；Power Blot Cassette仅用于转印（快速蛋白质转印），Power -stain Cassette仅用于蛋白质染色。我们优化了表面区域，可使每种应用获得最佳结果。较小的染色盒用于染色效果更好，而较大的转印盒用于转印效果更好。
不能。不可以在插入Power Blot Cassette时选择快速染色方案，反之亦然。
可以。Thermo Scientific Power Station可自动识别染色盒的插入并初始化正确的软件菜单系统。
Thermo Scientific Power System（货号22830）由带有激活版Staining和Blotting Software的Thermo Scientific Power Station（货号22838）、Thermo Scientific Power Stain Cassette（货号22836）以及Thermo Scientific Power Blot Cassette（货号22835）组成。该系统是Power Stainer和Power Blotter的组合。
Thermo Scientific Power Stainer（货号22833）由带有激活版Staining Software的Thermo Scientific Power Station（货号22838）以及Thermo Scientific Power Stain Cassette（货号22836）组成。该系统专为聚丙烯酰胺凝胶中蛋白质的快速Coomassie染色脱色而设计。传统Coomassie染色技术需要一小时至一夜的时间来完成染色和脱色，才能得到理想结果。与Thermo Scientific中型和小型凝胶快速染色试剂盒一起使用时，Thermo Scientific Power Stainer只需6分钟即可带来等同或优于传统Coomassie染色技术的染色效率。该系统将蛋白质固定在凝胶中，并通过电泳使带负电荷的Coomassie R250染料快速通过凝胶基质，从而实现蛋白质染色时间的显著缩短。染料通过聚丙烯酰胺并与蛋白质以离子键结合，产生亮蓝色条带和极低的背景。该系统已经过验证，对常用的预制和自制SDS-PAGE凝胶都适用。
Any staining procedure that uses over 50% alcohol will dehydrate a gel. Simply rehydrate the gel in some water.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No. Each cassette can only be used for its intended purpose; Power Blot Cassette for blotting (fast protein transfer) and Power Stain Cassette for protein staining. We have optimized the surface area to yield the best results for each application. The smaller stain cassette works better for staining while the larger blot cassette works better for blotting.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
No. It is not possible to insert the Power Blot Cassette and access the Power Staining protocol or vice versa.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes. The Thermo Scientific Power Station automatically recognizes the cassette that is inserted and initializes the correct software menu system.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Thermo Scientific Power System (Cat. No. 22830) consists of the Thermo Scientific Power Station (Cat. No. 22838) with activated Staining and Blotting Software, the Thermo Scientific Power Stain Cassette (Cat. No. 22836), and the Thermo Scientific Power Blot Cassette (Cat. No. 22835). It is a combination of the Power Stainer and the Power Blotter.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Thermo Scientific Power Stainer (Cat. No. 22833) consists of a Thermo Scientific Power Station (Cat. No. 22838) with activated Staining Software and a Thermo Scientific Power Stain Cassette (Cat. No. 22836). It is designed for rapid Coomassie staining of proteins in polyacrylamide gels and subsequent removal of stain from the gel background. Traditional Coomassie staining techniques require one hour to overnight of staining and destaining to achieve the desired results. When used in conjunction with Thermo Scientific Midi and Mini Gel Power Staining Kits, the Thermo Scientific Power Stainer is designed to provide staining efficiency in as few as 6 minutes that is equivalent to, or better than, traditional Coomassie staining techniques. This significant reduction in protein staining time is accomplished by fixing the protein to the gel and electrophoretically transporting the negatively charge Coomassie R250 dye rapidly through the gel matrix. The dye passes through the polyacrylamide and ionically binds to the protein, resulting in crisp blue bands with minimal background. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.