Pierce™ BCA 蛋白检测试剂盒 - 还原剂兼容
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Pierce™ BCA 蛋白检测试剂盒 - 还原剂兼容
Thermo Scientific™

Pierce™ BCA 蛋白检测试剂盒 - 还原剂兼容

该 BCA 蛋白检测试剂盒是我们广泛采用的 Pierce BCA 蛋白检测的还原剂兼容版本。使用该试剂盒,您可以测定含二硫化物还原剂的样品中的蛋白浓度。 比较所有可用 BCA了解更多信息
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货号数量
23250275 mL
货号 23250
价格(CNY)
4,596.00
Each
添加至购物车
数量:
275 mL
请求批量或定制报价
价格(CNY)
4,596.00
Each
添加至购物车
该 BCA 蛋白检测试剂盒是我们广泛采用的 Pierce BCA 蛋白检测的还原剂兼容版本。使用该试剂盒,您可以测定含二硫化物还原剂的样品中的蛋白浓度。

比较所有可用 BCA 蛋白检测›

BCA 蛋白检测试剂盒的特点—还原剂兼容包括:
兼容性—含高达 5 mM DTT、35 mM BME 或 10 mM TCEP
的检测样品•BCA 技术—仅对标准 BCA 蛋白检测方案进行了轻微修改(与兼容性试剂进行 15 min 孵育);无需沉淀步骤
样品体积— 仅需 25 μL(标准试剂盒)或小于 10 μL( 微孔板试剂盒)样品
比色—在 562 nm 下使用标准分光光度计或酶标仪测定
出色的均匀性—与染料结合方法相比表现出更少的蛋白间差异
检测范围—BSA 线性工作范围为 125 至 2000 μg/mL

BCA 蛋白检测试剂盒—还原剂兼容 (BCA-RAC) 提供了原始 BCA 检测的所有优势,以及与蛋白样品缓冲液中常规使用浓度的二硫化物还原剂的兼容性。这种 Pierce BCA 蛋白检测方法的特殊适用性可以对含有 DTT、BME 或 TCEP 的样品进行精确的蛋白浓度测定。这一还原剂兼容性 BCA 试剂盒扩展了 BCA 蛋白检测方案中已存在的广泛试剂兼容性,使得蛋白研究样品中常见的几乎所有类型的组分都包括在内。

相关产品
Pierce 微孔板 BCA 蛋白检测试剂盒 - 还原剂兼容
96 孔板用于 Pierce BCA-RAC 检测
仅供科研使用。不可用于诊断程序。
规格
检测BCA 蛋白定量:
描述Pierce BCA 管蛋白检测试剂盒 - 还原剂兼容 (RAC)
适用于(应用)基于溶液的检测、吸光度
适用于(设备)分光光度计、酶标仪
产品线Pierce™
产品类型蛋白定量检测
数量275 mL
特异性无靶标特异性
足够用于250 次试管检测
检测方法比色法
Unit SizeEach
内容与储存
足够用于:含 25 μL 样品的 250 管检测
•BCA 试剂 A,250 mL
• BCA 试剂 B,25 mL
•兼容性试剂,10 x 20 mg
•重组缓冲液,15 mL
•白蛋白标准品安瓿,2mg/mL, 10 x 1 mL

在室温下储存

常见问题解答 (FAQ)

Which Thermo Scientific protein assay is the best or the most reliable?

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can the compatibility reagent in the BCA-RACE kit be stored after reconstitution and reused?

The reconstituted compatibility reagent is stable for 8 hours when stored at 4 degrees C protected from light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many assays can be performed with each BCA-RAC kit?

The BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) has sufficient reagents for 250 samples or standards of 25µL each, and the Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) can be used to assay 1,000 samples or standards of 9µL each.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I increase the volumes of the sample and compatibility reagent in the microplate assay when using the BCA-RAC?

No. Increasing the sample and compatibility reagent volumes will require increasing the BCA working reagent, resulting in a volume that exceeds the well capacity. There must be space remaining in the well to allow mixing and prevent the solution from contacting the sealing tape.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What formats are available for the BCA-RAC?

There are two different formats: the BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) for microcentrifuge tube (1.5ml) assays and Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) for 96-well plates. The tube assay is compatible with 5mM DTT, 10mM TCEP and 35mM 2ME, and the microplate assay is compatible with 5mM DTT, 10mM TCEP and 25mM 2ME.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.