Pierce™ 比色蛋白酶检测试剂盒

Name: Pierce™ 比色蛋白酶检测试剂盒
SKU: 23263
Price: 5742.00 CNY

货号 23263

价格（CNY)

5,742.00

Each

添加至购物车

请求批量或定制报价

价格（CNY)

5,742.00

Each

添加至购物车

Thermo Scientific Pierce 比色蛋白酶检测试剂盒可测定样品中的总蛋白酶活性，提供评估蛋白酶分离程序进展或定量生物样品中的蛋白酶污染的方法。该试剂盒使用完全琥珀酰酪蛋白作为检测底物。在存在蛋白酶的情况下，该底物的水解导致含游离氨基末端基团的肽片段的释放。这些肽与三硝基苯磺酸 (TNBSA) 反应，随后测量由于黄色显色 TNB 肽加合物形成导致的吸光度增加。

比色蛋白酶检测试剂盒的特点：

•酪蛋白底物—测定任何将酪蛋白裂解为肽片段的蛋白酶的活性
•胰蛋白酶标准品—定量分析与胰蛋白酶（一种普遍认可的对比品）相关的蛋白酶活性
• 灵敏度—为使用未修饰形式酪蛋白的检测灵敏度的 1000 倍
•简单且快速—试管和微孔板方案在少于一小时内完成
•同质性—仅需添加步骤；无分离、转移或停止步骤
•可定制—易于适应时间、温度和 pH 值，以优化目标蛋白酶的灵敏度

应用：
•评估蛋白酶纯化的功能完整性和总体进展
•定量蛋白样品中的蛋白酶污染
•表征与胰蛋白酶相比的特殊蛋白酶的活性
•评价缓冲液条件对蛋白酶功能和活性的影响

额外质量标准：
•底物：琥珀酰酪蛋白（不单独提供）
•检测基础：检测因蛋白水解所致的伯胺
•检测试剂：三硝基苯磺酸 (TNBSA)
•测定：450 nm 处的吸光度（酶标仪或分光光度计）

相关产品
Pierce™ 荧光蛋白酶检测试剂盒
用于 Pierce™ 荧光蛋白酶检测试剂盒的 FITC 酪蛋白

For Research Use Only. Not for use in diagnostic procedures.

规格

检测蛋白酶检测

适用于（应用）吸光度

适用于（设备）微孔板读数仪

产品线Pierce™

产品类型蛋白活性检测

数量250 检测试剂盒

底物酪蛋白

底物属性基于蛋白的底物

底物类型蛋白酶底物

足够用于250 次微孔板检测

靶标蛋白酶

检测方法比色法

免疫测定试剂盒格式96 孔板

Unit SizeEach

常见问题解答 (FAQ)

In the Colorimetric Protease Assay Kit (Cat. No. 23263), my standard curve is not linear. Why?

Check the scale used for your X-axis. A logarithmic scale should be used for the X-axis (protease enzyme concentration) rather than a linear scale.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Colorimetric Protease Assay Kit (Cat. No. 23263), my sample absorbance values are lower than smallest trypsin or other protease standard values. Why?

It could be that the sample protease activity is low relative to the range of dilutions used for the standard curve. You can prepare and use additional standard dilutions to ensure that the full range of assay sensitivity is utilized. Alternatively, the sample protease activity is low relative to the protease used to make standard curve. You can prepare a standard curve using the known amounts of the specific protease being tested.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Colorimetric Protease Assay Kit (Cat. No. 23263), all of my sample absorbance values for non-trypsin proteases are low. What can I do?

There are two potential causes for low absorbance values in the unknown samples. The first being that pH and other buffer conditions were not optimal for the protease being tested. You can repeat the assay using buffer conditions that are optimal for the protease being tested and used to make standard curve. Secondly, it is possible that the protease being tested digests the casein substrate more slowly or less fully than the trypsin standard for which the default assay method was developed. You can perform the digestion step for >20 minutes (e.g., 40 mins); if necessary, also perform the TNBSA development step for longer. You can also use the specific substrate for the protease being tested.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you offer any protein assays that are protein specific?

We offer several “specialty” protein assays including: Protease assay kits (Cat. Nos. 23263 and 23266), Glycoprotein Carbohydrate Estimation Kit (Cat. No. 23260), Phosphoprotein Phosphate Estimation Kit (Cat. No. 23270), Quantitative Peroxide assay kits (Cat. Nos. 23280 and 23285), and Easy-Titer Assay kits for IgG and IgM.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.