Pierce™ 定量肽检测试剂与标准品
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Pierce™ 定量肽检测试剂与标准品
Thermo Scientific™

Pierce™ 定量肽检测试剂与标准品

Pierce 比色或荧光定量肽检测试剂和标准品是易于使用的微孔板检测试剂,专为提高与质谱分析配合使用的肽定量的灵敏度和可重现性而设计。
Have Questions?
更改视图buttonViewtableView
货号产品类型检测方法
23275肽检测比色法
23290肽检测荧光
23295肽酶切检测标准品比色, 荧光
货号 23275
价格(CNY)
4,029.00
飞享价
Ends: 31-Dec-2025
5,218.00
共减 1,189.00 (23%)
Each
添加至购物车
产品类型:
肽检测
检测方法:
比色法
请求批量或定制报价
价格(CNY)
4,029.00
飞享价
Ends: 31-Dec-2025
5,218.00
共减 1,189.00 (23%)
Each
添加至购物车
Pierce 比色或荧光定量肽检测试剂是一种简单的混合读取微孔板检测试剂,具有稳定的信号,可准确、可靠地测定肽消化样品。优质的肽消化参考标准品单独提供或包含在试剂盒内,用于生成线性标准曲线,以提高准确性和可重现性。这款灵敏度更高、测定样品量较低且随附的参考标准品至关重要,它可准确、可靠地测定肽消化样品,进而对进样量进行归一化,以便用于 MS 分析。
灵敏 — 准确检测低至 5 µg/mL(荧光法)或 25 µg/mL(比色法)的肽混合物
可重现 — 检测试剂盒的性能已使用肽消化混合物进行严格检测
可靠的肽消化标准品 — 试剂盒包括一种经验证的肽消化标准品,可提高定量的可重现性
兼容 — 可与多种试剂配合使用,包括 MS 样品制备试剂和 TMT 标记肽(荧光测定)
方便 — 简单的混合读取形式,在短短5分钟(荧光法)或15分钟(比色法)内便可读到稳定信号

Pierce 定量比色肽检测试剂盒提供了改良的 BCA 试剂(用于将 Cu+2 还原为 Cu+1)、经优化的专有螯合剂(用于定量肽混合物)以及肽消化参考标准品(用于生成对照线性标准曲线)。这款比色肽检测试剂盒只需少量样品 (20 µL),且工作肽浓度范围为 25–1000 μg/mL。在反应中,先在碱性条件下通过肽的酰胺主链还原铜(双缩脲反应),随后,还原的铜与螯合物结合形成明亮的红色复合物(吸收波长为 480 nm),在短短15分钟内便可读取到该复合物。该反应产生的信号比用于肽分析的 Micro-BCA 蛋白检测试剂盒灵敏 3–4 倍。比色肽检测试剂盒与 TMT 标记肽兼容,但不建议用于合成肽,因为该检测试剂盒会受到肽氨基酸含量的影响。

Pierce 定量荧光肽检测试剂盒的试剂包括肽测定缓冲液、荧光肽标记试剂以及用于肽浓度定量测定的肽消化测定标准品。这款灵敏的检测试剂盒仅需 10 µL 样品,会随着肽浓度增加 (5–1000 μg/mL) 而产生线性响应,并且可生成在短短5分钟内便能检测到的稳定最终荧光。在此检测试剂盒中,使用胺反应性荧光试剂(直到与胰蛋白酶肽反应才会发出荧光)在肽的氨基末端添加了特异性标记。在微孔板读数仪 (ex390/em475) 上检测荧光标记的肽。该检测试剂盒适用于定量测定肽消化混合物和氨基末端未封闭的合成肽。荧光检测试剂盒与 TMT 标记肽不兼容。

Thermo Scientific 肽消化测定标准品旨在用作与 Pierce 定量荧光和比色肽检测试剂配合使用的参考标准品,以提高肽定量的可重现性和准确性。肽消化测定标准品以浓度为 1 mg/mL 的即用型液体规格提供。已使用 MS 级胰蛋白酶消化参考蛋白,以尽量减少缺失的切割。为确保性能一致,监测蛋白的酶切效率以确保批间一致性,并通过与参比标准品进行比较来评估质量。

应用
• 对样品浓度进行归一化,以便用于下游定量应用
• 对上样量进行归一化,以便用于 LC、MS 和 LC/MS 应用
• 在肽纯化步骤后测量回收率

For Research Use Only. Not for use in diagnostic procedures.
规格
最终产品类型
适用于(设备)微孔板读数仪
数量500 次检测
工作流程步骤过程中检测
检测方法比色法
产品规格试剂盒
产品线Pierce™
产品类型肽检测
原始材料肽, 蛋白酶酶切的蛋白
Unit SizeEach

常见问题解答 (FAQ)

In the SMOAC protocol (https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/10/High-SelectTM-SMOAC-Protocol.pdf), can I enrich with High-Select Fe-NTA kit first?

No. It is important to enrich with the TiO2 kit (Cat. No. A32993) first. Afterwards, the flow-through and wash fractions from this enrichment can be processed with the Fe-NTA kit (Cat. No. A32992). If this order is reversed (that is, Fe-NTA before TiO2), there will be 2 consequences as follows: 1. There will not be any significant additional recovery of peptides (maybe just a few more peptides). 2. There will be no enrichment for the multiple phosphorylated peptides, so those would be lost.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why do you offer two phosphopeptide enrichment kits: the High-Select TiO2 kit (Cat. No. A32993) and the High-Select Fe-NTA kit (Cat. No. A32993)?

The two phosphopeptides enrichment kits, Fe-NTA and TiO2, enrich a complementary set of phosphopeptides. Our R&D has developed a Sequential enrichment of Metal Oxide Affinity Chromatography (see https://assets.thermofisher.com/TFS-Assets/CMD/posters/PO-65032-SMOAC-Phosphoproteomics-Peptides-ASMS2017-PO65032-EN.pdf and https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/10/High-SelectTM-SMOAC-Protocol.pdf?CID=bid_mic_r04_jp_cp0000_pjt0000_bid00000_0so_blg_protein_analysis_mass_spectrometry_bid_ts_mbr_24065_Social_LAB) where flow-through and wash fractions from TiO2 enrichment were combined and subjected to Fe-NTA enrichment. This sequential enrichment provides impressive coverage of phosphoproteomes.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the differences between the old Fe-NTA kit (Cat. No. 88300) and the new High-Select Fe-NTA kit (Cat. No. A32992)?

There are four differences between the Fe-NTA kit (Cat. No. 88300) and the new High-Select Fe-NTA kit (Cat. No. A32992) kit as follows: 1. The selectivity - the ratio of number of phosphopeptides over total peptides - was significantly improved to 99% with Cat. No. A32992, because the reagents were extensively optimized for the phosphopeptide selection. 2. The phosphopeptide yield was also increased to 33 µg based on quantitative colorimetric peptide assay (Cat. No. 23275). 3. The reagent is a pre-formulated format, so mixing reagent to prepare the working solution from stock solutions provided in the old kit (Cat. No. 88300) is not necessary, so it is easier to handle. 4. The enrichment protocol is optimized and streamlined, which means there are many fewer steps than with Cat. No. 88300. Thus, it takes <45 min to finish the entire protocol compared to 2 hours with the old kit (Cat. No. 88300).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the standard solvent for the Pierce Quantitative Peptide Assays & Standards (Cat. No. 23275)?

The solvent for the Pierce Quantitative Peptide Assays & Standards (Cat. No. 23275) is 50mM ammonium bicarbonate.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

When I quantitate my mass spec peptide sample with the Pierce Quantitative Colorimetric Peptide Assay, I get different results than when I use the Pierce Quantitative Fluorometric Peptide Assay. Which is best to use for the most accurate quantitation?

Since the different peptide assays use different chemistries to measure peptides, they may result in different results. Interfering compounds are the most common source of background and inaccurate measurements. Please note that the fluorometric peptide assay is not recommended for peptides which have been modified using TMT reagents.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.