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View additional product information for KingFisher™ Flex Purification System - FAQs (24074441, 24075410, 24074431, 24074420, 5400620, A39966, A39952, 24074410, 24074421, 24074411)
38 product FAQs found
不是，处理体积指的是24孔深孔板内可以装入的最大体积。这包括结合缓冲液的体积和磁珠体积，所以当扩大反应体积时，一定要确保总体积不超过5mL。
这种情况有时会发生，但是这不会影响产量，因为样本已经从磁珠上被洗脱下来了。
如果没有向磁棒套架插入磁棒套，则程序不会开始运行。
将软布或纸巾用温和的去垢剂、肥皂水或酒精润湿，然后擦拭磁力棒。
可以通过运行“维护 (Maintenance)”菜单之下的“更换磁头 (Change magnet)”程序实现，使用“上 (up)”和“下 (down)”按钮进行选择。
The KingFisher Flex磁体是使用非常稳定的材料制成的。磁场不会变弱。不过，极端的机械外力或加热可能会对磁体造成损坏。
注：务必使KingFisher Flex的磁棒头远离其他磁棒或其他磁体。磁体相互接触会引起严重损坏。不行，我们强烈推荐您使用MagMAX板或KingFisher板，因为它们是专为配合KingFisher Flex磁棒套同时使用而设计的，以达到最佳效果。其它制造商生产的板子可能与KingFisher Flex加热模块不兼容。它们还可能引起意料之外的问题，例如可能会由于不同的孔容积和板底高度而引起交叉污染。
带96深孔磁棒头的KingFisher Flex仪器和MagMAX Express-96仪器所需的耗材是相同的。列表如下：
- MagMAX Express-96 深孔板 (货号 4388476)
- MagMAX Express-96 标准板(货号4388475)
- MagMAX Express-96深孔磁棒套(货号4388487)
- MicroAmp 透明封口膜（可选）： (货号. 4306311)
对于带24深孔磁棒头的KingFisher Flex仪器，所需耗材列表如下：
- KingFisher Flex 24深孔板：(货号. 95040480)
- KingFisher Flex 24深孔磁棒套： (货号 97002610)
KingFisher Flex 磁珠处理仪有四种磁棒选择：
- 用于MagMAX Express-96深孔板的KingFisher Flex磁棒
- 用于KingFisher 24深孔板的KingFisher Flex磁棒
- 用于KingFisher 96孔板的KingFisher Flex磁棒
- 用于有裙边PCR板的KingFisher Flex磁棒
是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。
The accuracy of the KingFisher Flex heating block is ± 1°C for up to 80°C, and ± 2°C for up to 115°C (see page 72 of the manual). If there is an issue with the accuracy of the heating block, you can contact to schedule a service call. Calibration of the heating block can only be performed by one of our engineers.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
The KingFisher Flex Purification System heating block is located inside the instrument and can be used automatically during the protocol. All KingFisher Flex plates can be heated using specially designed, interchangeable heating blocks. Any number of heating steps can be added to a protocol. When a protocol enters a heating step, the plate is automatically moved to the dedicated heating position for heating. Afterwards, the protocol continues automatically to the next step.
The only way is to do this is by rewriting the script from where you want it to start, or start the run all over again.
There is no way to edit a BindiT protocol that has been installed on the KingFisher Flex/KingFisher DuoPrime instrument. The only way to edit the protocol is by saving the original .bdz file to a PC and then editing it in BindiT software.
Any kind of protein purification can be done on the KingFisher Flex or Duo Prime as long as it is based on magnetic beads. Some template protocols for different magnetic beads are available for the KingFisher Flex and Duo Prime at this website: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/automated-protocols-software.html?open=protein.
Please know that these templates are only a starting point and each protein and application is unique so optimization will be required. This optimization and protocol modification is the responsibility of the end user.
The best place to download KingFisher Flex and KingFisher Duo Prime protocols for a particular MagMAX kit is the relevant MagMAX kit product page. The Bindlt script protocol (.bdz) files can be found under the Documents>Product literature section.
No, the processing volume refers to the maximum volume that can be held in the 24 Deep Well plates. This incorporates the volume of binding buffers and beads, so when scaling up reaction volumes, it is important to make sure that the total volume does not exceed 5 mL.
No, once a protocol was stopped, it will restart from the beginning. The extraction procedure can either be finished manually by using a magnetic stand, or a modified version of the original KingFisher Flex script would have to be created that starts off at the point where the run was stopped.
Set up the protocol on the BindIt software. After the first 8 plates are done, the instrument will pause and instruct you to switch out the plates.
The transport locks are only necessary when relocating the instrument.
We strongly recommend that you maintain the specified volumes within the defined limits to avoid spillover and to help ensure the best performance at the most efficient level.
This happens sometimes but it will not affect the yield because the sample has been released from the particles.
If the starting material is too viscous, the magnetic rods will not be able to collect the particles. Dilute the sample and check that the sample is properly homogenized/lysed. Adding low amounts of detergent will improve the collection of magnetic particles as well. Quickly centrifuge the plate to sediment the particles to the bottom of the plate.
The protocol will not start without the tip combs inserted into the tip comb holder.
Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution, soap solution, or alcohol.
The heating block warms up at the rate of about 10°C per minute, so it will take about 6 minutes.
Both deep-well plates and KingFisher 96 plates can be used during the same run. Therefore, it is possible to start the processing by using larger volumes (in a deep-well plate) and elute the purified sample to a smaller volume (in a KingFisher 96 or MagMAX 96 standard plate).
This can be done by running the Change magnet protocol under the Maintenance menu using the “up” and “down” cursor keys.
The KingFisher Flex magnets are made of material that is very stable. The magnetic field will not get weaker. However, extreme mechanical force or heating can cause damage to the magnets.
Caution: It is very important to keep the KingFisher Flex heads away from each other and from other magnets at all times. Clashing together of the magnets may cause serious damage to the magnets.
KingFisher Flex uses a USB port, whereas the MagMAX Express- 96 requires a RS-232 port.
No. Protocols cannot be transferred back from the instrument to the computer. Protocols need to be previously stored in the BindIt or KingFisher software to make edits.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.
No, we strongly recommend that you use the MagMAX plates or KingFisher plates since they were specifically designed to be used with KingFisher Flex tip combs to attain maximal performance. Plates from other manufacturers may not be compatible with the KingFisher Flex heating blocks. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.
There are 4 magnetic head options available for the KingFisher Flex Magnetic Particle Processor:
- KingFisher Flex head for MagMAX Express-96 Deep Well plate
- KingFisher Flex head for KingFisher 24 Deep Well plate
- KingFisher Flex head for KingFisher 96 plate
- KingFisher Flex head for PCR plate, skirted
Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.