Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
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Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Citations & References (5)
Thermo Scientific™

Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters forRead more
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Catalog NumberQuantity
24510500 mg
A3926910 x 2 mg
245255 g
Catalog number 24510
Price (CNY)
4,008.00
Each
Add to cart
Quantity:
500 mg
Request bulk or custom format
Price (CNY)
4,008.00
Each
Add to cart
Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.

Features of Sulfo-NHS:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
• Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule
• Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-impermable (can be used for cell surface labeling)
• High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives

Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Applications:
• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive Sulfo-NHS esters
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive Sulfo-NHS esters

Specifications for Sulfo-NHS:
We manufacture N-hydroxysulfosuccinimide to the highest possible specifications to produce the most specific bioconjugates, to ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-NHS is tested to meet the following minimum specifications:

• Purity—Greater than 95% by quantitative NMR (the highest standard for crosslinker purity);
average lot purity is greater than 99%
• Solubility—Sample dissolves at 2 mg/mL in deionized water to yield a clear, colorless solution
• Identity—IR scan shows only peaks characteristic of N-hydroxysulfosuccinimide

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)
NHS (N-hydroxysuccinimide)
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityNo
DescriptionSulfo-NHS
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)217.13
PEGylatedNo
Product LinePierce
Quantity500 mg
Reactive MoietyCarbodiimide
Shipping ConditionAmbient
SolubilityWater
Water SolubleYes
Chemical ReactivityAmine-Carboxyl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard, Single-use, Premium-grade
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

Can NHS and EDC stock solutions be made for long-term storage before use?

No. EDC is very unstable in aqueous environments and must be dissolved immediately before use. NHS and Sulfo-NHS are relatively stable in solution but best results are obtained when they are dissolved immediately before use. Store these compounds desiccated at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are there any substances that interfere with NHS reactions?

Yes. Except for the intended targets, reactions must not contain carboxyl or amine compounds. Thus, Tris, glycine, lysine, ethanolamine or other amine- containing buffers must be avoided.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between NHS and Sulfo-NHS?

Sulfo-NHS is the sulfonate sodium salt of NHS; it is water-soluble, but not membrane-permeable. NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical. Sulfo-NHS is chosen for its ability to confer better solubility to the activated compound and/or to control its membrane permeability.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How are NHS and Sulfo-NHS used?

These compounds are used to modify a carboxyl group to form an amine-reactive ester. This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat. No. 22980, 22981, 77149, A35391). EDC causes a dehydration reaction between the carboxyl and the NHS hydroxyl group, giving rise to an NHS-ester-activated molecule. The activated molecule can then be reacted spontaneously with a primary amine-containing molecule. Although the carboxyl-molecules can be made to react directly with amines using EDC, the reaction is much more efficient with NHS because a stable intermediate is created. In fact, molecules that are activated as NHS esters can be dried and stored for later reaction to amine-containing targets.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are NHS and Sulfo-NHS used for?

These compounds are used in conjunction with the crosslinker EDC (Cat. No. 22980, 22981, 77149, A35391) to synthesize amine-reactive labeling reagents, crosslinkers and conjugation compounds. Any compound containing a carboxylic acid (-COOH), such as a protein, or biotin or peptide, can be activated with NHS or Sulfo-NHS to form an NHS ester that will spontaneously react to form covalent amide linkages with proteins and other molecules that contain primary amines (-NH2).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Sulfo-NHS (N-hydroxysulfosuccinimide), 500 mg FAQs

Citations & References (5)

Citations & References
Abstract
Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces.
Authors:Guha Thakurta S, Miller R, Subramanian A
Journal:J Biomater Appl
PubMed ID:20819918
'The response of biomaterial surfaces when exposed to blood is in part dependent upon the nature and composition of the adsorbed layer of proteins. Surfaces passivated with albumin have been shown to reduce platelet adhesion and activation. In an attempt to develop surfaces that can selectively and specifically bind albumin, ... More
Mouse monoclonal antibodies to anthrax edema factor protect against infection.
Authors:Leysath CE, Chen KH, Moayeri M, Crown D, Fattah R, Chen Z, Das SR, Purcell RH, Leppla SH
Journal:Infect Immun
PubMed ID:21911463
'Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the ... More
Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
Authors:Bergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A
Journal:J Biol Chem
PubMed ID:22692205
'The two major mammalian sialic acids are N-acetylneuraminic acid and N-glycolylneuraminic acid (Neu5Gc). The only known biosynthetic pathway generating Neu5Gc is the conversion of CMP-N-acetylneuraminic acid into CMP-Neu5Gc, which is catalyzed by the CMP-Neu5Ac hydroxylase enzyme. Given the irreversible nature of this reaction, there must be pathways for elimination or ... More
Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles.
Authors:Sperling RA, Parak WJ
Journal:Philos Trans A Math Phys Eng Sci
PubMed ID:20156828
Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the ... More
The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II.
Authors:Ido K, Kakiuchi S, Uno C, Nishimura T, Fukao Y, Noguchi T, Sato F, Ifuku K
Journal:J Biol Chem
PubMed ID:22707728
The PsbP protein regulates the binding properties of Ca(2+) and Cl(-), and stabilizes the Mn cluster of photosystem II (PSII); however, the binding site and topology in PSII have yet to be clarified. Here we report that the structure around His-144 and Asp-165 in PsbP, which is suggested to be ... More