Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces.
AuthorsGuha Thakurta S, Miller R, Subramanian A
JournalJ Biomater Appl
PubMed ID20819918
'The response of biomaterial surfaces when exposed to blood is in part dependent upon the nature and composition of the adsorbed layer of proteins. Surfaces passivated with albumin have been shown to reduce platelet adhesion and activation. In an attempt to develop surfaces that can selectively and specifically bind albumin, ... More
Mouse monoclonal antibodies to anthrax edema factor protect against infection.
AuthorsLeysath CE, Chen KH, Moayeri M, Crown D, Fattah R, Chen Z, Das SR, Purcell RH, Leppla SH
JournalInfect Immun
PubMed ID21911463
'Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the ... More
Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
AuthorsBergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A
JournalJ Biol Chem
PubMed ID22692205
'The two major mammalian sialic acids are N-acetylneuraminic acid and N-glycolylneuraminic acid (Neu5Gc). The only known biosynthetic pathway generating Neu5Gc is the conversion of CMP-N-acetylneuraminic acid into CMP-Neu5Gc, which is catalyzed by the CMP-Neu5Ac hydroxylase enzyme. Given the irreversible nature of this reaction, there must be pathways for elimination or ... More
Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles.
AuthorsSperling RA, Parak WJ
JournalPhilos Trans A Math Phys Eng Sci
PubMed ID20156828
Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the ... More
The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II.
AuthorsIdo K, Kakiuchi S, Uno C, Nishimura T, Fukao Y, Noguchi T, Sato F, Ifuku K
JournalJ Biol Chem
PubMed ID22707728
The PsbP protein regulates the binding properties of Ca(2+) and Cl(-), and stabilizes the Mn cluster of photosystem II (PSII); however, the binding site and topology in PSII have yet to be clarified. Here we report that the structure around His-144 and Asp-165 in PsbP, which is suggested to be ... More