该膜染色试剂盒采用一种无损、可逆、可靠且灵敏的方法来染色和检测硝酸纤维素膜上的蛋白质。使用该方法,每条带检测下限为 25 至 50 ng (敏感度比传统丽春红 S 染料至少高 5 倍)。这一染料与膜表面和蛋白质转印试剂的非特定相互作用最小。染色方案简单、快速,可产生不褪色的蓝绿色条带,且易于拍照以备将来参考。染料可在 15 分钟内轻松逆转。不会影响后续的免疫印迹检测,因为染料不会改变蛋白质,并会被完全去除。此外,该染色与膜分离和洗脱蛋白的 N 端序列分析兼容。
仅供科研使用。不可用于诊断程序。
规格
描述适合硝化纤维膜的 Pierce 可逆蛋白质染色试剂盒
检测定位印迹检测
检测方法比色法
产品线Pierce™
产品类型可逆性蛋白染色试剂盒
数量1.5 L
靶标分子蛋白质
标签或染料具有专利技术的预混液
Unit SizeEach
内容与储存
足够用于:10 支小型凝胶 (SDS-PAGE) 中的硝酸纤维膜 (8 cm x 8 cm) •可逆染色,250 mL • 脱色,2 x 500 mL • 染色去除剂,250 mL
在室温下储存
常见问题解答 (FAQ)
How can I increase the signal intensity when I am using SuperSignal West Femto Maximum Sensitivity Substrate?
The following are possible causes for weak signal intensity:
- Low level of protein transferred from the gel to the membrane. This is particularly true for high molecular weight proteins. Reversible Protein Stain Kits for Nitrocellulose and PVDF membranes (Cat. Nos. 24580 and 24585, respectively) may be used to stain the bands on the membrane to indicate transfer efficiency.
- Over-diluted primary and secondary antibody probes. We suggest using the recommended dilution for the antibody probes for each substrate. More sensitive substrates will require a more dilute antibody solution.
- Short substrate incubation time. For best results, incubate the membrane for 5-15 min in the SuperSignal substrate.
How can I increase the signal intensity when I am using SuperSignal West Dura Extended Duration Substrate?
The following are possible causes for weak signal intensity:
- Low level of protein transferred from the gel to the membrane. This is particularly true for high molecular weight proteins. Reversible Protein Stain Kits for Nitrocellulose and PVDF membranes (Cat. Nos. 24580 and 24585, respectively) may be used to stain the bands on the membrane to indicate transfer efficiency.
- Over-diluted primary and secondary antibody probes. We suggest using the recommended dilution for the antibody probes for each substrate. More sensitive substrates will require a more dilute antibody solution.
- Short substrate incubation time. For best results, incubate the membrane for 5-15 min in the SuperSignal substrate.