GelCode™ 蓝色染色试剂
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GelCode™ 蓝色染色试剂
Thermo Scientific™

GelCode™ 蓝色染色试剂

GelCode Blue 染色试剂是一种基于胶体考马斯染料 G-250 的即用型蛋白染色剂,可提供聚丙烯酰胺凝胶中的纳克级检测以及印迹中检测。特点:•无需进行甲醇-乙酸脱色• 快速一步法一小时染色—即用型染色试剂;可直接在染色托盘中观察到条带形成;无过夜染色产生的背景;灵活的固定和清洗方案;无凝胶收缩;可以对染色凝胶进行干燥处理• 水洗提高了染色灵敏度—可选步骤提供了水晶般透明凝胶背景,使弱染色的条带变得更易见比较所有考马斯染料 ›这一独特的 GelCode了解更多信息
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货号数量
24590500 mL
货号 24590
价格(CNY)
320.00
飞享价
Ends: 31-Dec-2025
1,271.00
共减 951.00 (75%)
Each
添加至购物车
数量:
500 mL
请求批量或定制报价
价格(CNY)
320.00
飞享价
Ends: 31-Dec-2025
1,271.00
共减 951.00 (75%)
Each
添加至购物车
GelCode Blue 染色试剂是一种基于胶体考马斯染料 G-250 的即用型蛋白染色剂,可提供聚丙烯酰胺凝胶中的纳克级检测以及印迹中检测。

特点:
•无需进行甲醇-乙酸脱色
快速一步法一小时染色—即用型染色试剂;可直接在染色托盘中观察到条带形成;无过夜染色产生的背景;灵活的固定和清洗方案;无凝胶收缩;可以对染色凝胶进行干燥处理
水洗提高了染色灵敏度—可选步骤提供了水晶般透明凝胶背景,使弱染色的条带变得更易见

比较所有考马斯染料 ›

这一独特的 GelCode 蓝色染色试剂仅对蛋白染色,支持在一小时凝胶染色过程中直接在凝胶中观察条带。由于脱色步骤不能固定蛋白,GelCode 蓝色染色剂与质谱分析和 N 端序列分析兼容。
仅供科研使用。不可用于诊断程序。

GelCode 蓝色染色试剂包含添加剂,有助于减慢染料-染料和染料-蛋白聚合物的形成,该聚合物形成于所有基于考马斯染料的蛋白染色试剂中。然而,如果不进行干扰,该试剂将形成可见染料-染料聚合物并在瓶底沉淀。幸运的是,将瓶子轻轻翻转或斜晃几次可使这些聚合物完全分散。因此,在倾倒或分配之前最好将染色试剂混合,以确保使用均匀的试剂样品。

产品参考资料:

  1. Mateer, S.C.,et al.(2002).J.Biol.Chem.227,12324-12333.
  2. Aulak, K.S.,et al.(2001).Proc.Natl.Acad.Sci.USA98,12056-12061.
  3. Lim, J.,et al.(2002).J.Biol.Chem.277,20774-20782.
  4. Hilton, J.M.,et al.(2001).J.Biol.Chem.276,16341-16347.
  5. Tani, M.,et al.(2002).J.Biol.Chem.275,3462-3468.
规格
检测定位印迹内检测,凝胶内检测
检测方法比色法
产品线GelCode™
产品类型蛋白凝胶染色试剂
数量500 mL
有效期每 6 个月
靶标分子蛋白质
颜色Blue
标签或染料Coomassie
Unit SizeEach
内容与储存
储存于 4°C。

常见问题解答 (FAQ)

我能否在免疫印迹分析前,使用胶体蓝染料对凝胶进行染色?

可以,胶体蓝染料可在免疫印迹前使用。但是,为得到理想的转印效率,我们建议进行凝胶脱色处理,然后在一系列Tris 碱/甘氨酸/SDS溶液中平衡,增加染料的溶解;转印完成时,应使用甲醇处理膜,从而在之前去除染料[从而在化学发光显影前去除染料](不需要在化学发光检测前进行脱色处理)。

Does GelCode Blue Stain Reagent contain coomassie G-250 or R-250 dye and what is the difference?

GelCode Blue Stain Reagent contains coomassie G-250 dye. Coomassie G (green)-250 is greenish, stains bright blue, is less soluble and used in colloidal form (stain enters a protein better than the acrylamide, so background is minimized). Coomassie R (red)-250 dye is reddish, stains dark blue and is more soluble, usually resulting in high background.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is there a faster staining method using GelCode Blue Stain Reagent?

Yes. The product instructions include a microwave procedure for faster staining with only a minimal loss in sensitivity. Protein bands can be detected approximately 30 minutes after electrophoresis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do any gel types require fixing before staining with the GelCode Blue Stain Reagent?

Yes. For gels electrophoresed with MOPS or MES running buffers, fix gel with 50% methanol and 7% acetic acid for 15 minutes and extensively wash with water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can a GelCode Blue Reagent-stained gel also be stained with silver stain?

Yes. The GelCode Blue Reagent-stained gel must be fixed overnight with 30% methanol and 10% acetic acid, followed by washing with water for 1 hour; then the gel is then ready for staining with a silver stain (e.g., Cat. No. 24612).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.