我能否在免疫印迹分析前,使用胶体蓝染料对凝胶进行染色?
可以,胶体蓝染料可在免疫印迹前使用。但是,为得到理想的转印效率,我们建议进行凝胶脱色处理,然后在一系列Tris 碱/甘氨酸/SDS溶液中平衡,增加染料的溶解;转印完成时,应使用甲醇处理膜,从而在之前去除染料[从而在化学发光显影前去除染料](不需要在化学发光检测前进行脱色处理)。
Does GelCode Blue Stain Reagent contain coomassie G-250 or R-250 dye and what is the difference?
GelCode Blue Stain Reagent contains coomassie G-250 dye. Coomassie G (green)-250 is greenish, stains bright blue, is less soluble and used in colloidal form (stain enters a protein better than the acrylamide, so background is minimized). Coomassie R (red)-250 dye is reddish, stains dark blue and is more soluble, usually resulting in high background.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Is there a faster staining method using GelCode Blue Stain Reagent?
Yes. The product instructions include a microwave procedure for faster staining with only a minimal loss in sensitivity. Protein bands can be detected approximately 30 minutes after electrophoresis.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Do any gel types require fixing before staining with the GelCode Blue Stain Reagent?
Yes. For gels electrophoresed with MOPS or MES running buffers, fix gel with 50% methanol and 7% acetic acid for 15 minutes and extensively wash with water.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Can a GelCode Blue Reagent-stained gel also be stained with silver stain?
Yes. The GelCode Blue Reagent-stained gel must be fixed overnight with 30% methanol and 10% acetic acid, followed by washing with water for 1 hour; then the gel is then ready for staining with a silver stain (e.g., Cat. No. 24612).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.