Diversity of human small intestinal Streptococcus and Veillonella populations.
Authorsvan den Bogert B, Erkus O, Boekhorst J, de Goffau M, Smid EJ, Zoetendal EG, Kleerebezem M
JournalFEMS Microbiol Ecol
PubMed ID23614882
'Molecular and cultivation approaches were employed to study the phylogenetic richness and temporal dynamics of Streptococcus and Veillonella populations in the small intestine. Microbial profiling of human small intestinal samples collected from four ileostomy subjects at four time points displayed abundant populations of Streptococcus spp. most affiliated with S. salivarius, S. thermophilus, ... More
Determine the effect of p53 on chemosensitivity.
AuthorsSenturk E, Manfredi JJ
JournalMethods Mol Biol
PubMed ID23150441
'The p53 tumor suppressor protein plays a central role in mediating the cellular response to a variety of stresses. Activation of p53 signaling will trigger cell cycle arrest or apoptosis in normal cells, depending on such factors as cell type and genetic context. The ability of a cell to circumvent ... More
Visualization of fas-mediated death-inducing signaling complex formation by immunoprecipitation.
AuthorsCordeiro N, Bidère N
JournalMethods Mol Biol
PubMed ID23397387
The prototypical death receptor Fas (also known as CD95 or Apo-1) plays an essential role in the maintenance of lymphocyte homeostasis. Propagation of cell death through Fas relies on the formation of a multiprotein complex at the receptor level known as the death-inducing signaling complex (DISC). Here, we describe an ... More
Collagen I confers gamma radiation resistance.
AuthorsAzorin E, González-Martínez PR, Azorin J
JournalAppl Radiat Isot
PubMed ID22705234
The effect of collagen on the response of somatomammotroph tumor cells (GH3) to gamma, radiation therapy was studied in vitro. After incubating confluent GH3 cell monolayers in a serum-free, maintaining medium, either with or without collagen, the monolayers were irradiated with 137Cs, gamma radiation. Collagen reduces cell mortality via ERK1/2 ... More
Microarray-based CGH and copy number analysis of FFPE samples.
AuthorsAl-Mulla F
JournalMethods Mol Biol
PubMed ID21370011
Over the past decade, utilization of microarray technology has flourished in biomedical research. It has evolved rapidly into a revolutionary tool that offers deeper insight into the molecular basis associated with complex diseases, especially in the field of cancer. Specifically, array-based Comparative Genomic Hybridization (aCGH) permits the detection of genome-wide ... More
Separation of single-stranded DNA, double-stranded DNA and RNA from an environmental viral community using hydroxyapatite chromatography.
Viruses, particularly bacteriophages (phages), are the most numerous biological entities on Earth. Viruses modulate host cell abundance and diversity, contribute to the cycling of nutrients, alter host cell phenotype, and influence the evolution of both host cell and viral communities through the lateral transfer of genes. Numerous studies have highlighted ... More