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Citations & References (10)
Gibco™
L-Glutamine (200 mM)
Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for a variety of critical cellular processes.
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| Catalog Number | Quantity |
|---|---|
| 25030081 | 100 mL |
| 25030149 | 20 mL |
| 25030164 | 20 x 100 mL |
Catalog number 25030081
Price (CNY)
497.00
Online Exclusive
Ends: 27-Dec-2025
688.00Save 191.00 (28%)
Each
Quantity:
100 mL
Price (CNY)
497.00
Online Exclusive
Ends: 27-Dec-2025
688.00Save 191.00 (28%)
Each
Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution with a rate of degradation dependent upon time, temperature, and pH. The optimal concentration depends upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.
Gibco L-Glutamine supplement is a ready-to-use 200 mM stock solution for cell culture supplementation that supports of many critical cellular functions, including:
- Formation of purine and pyrimidine nucleotides
- Formation of amino sugars, glutathione, and L-glutamate
- Formation of other amino acids
- Protein synthesis
- Glucose production
cGMP manufacturing and quality system
For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Specifications
Chemical Name or MaterialGlutamine
Concentration or Composition (by Analyte or Components)100X
Green FeaturesSustainable packaging
Physical FormLiquid
Product LineGibco
Recommended StorageStorage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
Shelf Life24 Months
Shipping ConditionDry Ice
Quantity100 mL
pH5 to 6
Unit SizeEach
Frequently asked questions (FAQs)
Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?
Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?
My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?
What is your recommended method for thawing L-Glutamine before adding to cell culture media?
Why did my 200 mM L-glutamine precipitate when I thawed it?
Citations & References (10)
Citations & References
Abstract
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media.